##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545520_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2847052
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07616615362136	31.0	31.0	33.0	30.0	34.0
2	31.342682536181286	31.0	31.0	34.0	30.0	34.0
3	31.432443102549584	31.0	31.0	34.0	30.0	34.0
4	35.190424340686434	37.0	35.0	37.0	32.0	37.0
5	35.054583477927345	37.0	35.0	37.0	32.0	37.0
6	35.10641147404403	37.0	35.0	37.0	32.0	37.0
7	35.75527212007368	37.0	35.0	37.0	35.0	37.0
8	35.764762638687316	37.0	35.0	37.0	35.0	37.0
9	37.45044804239613	39.0	37.0	39.0	35.0	39.0
10	36.66663306465776	39.0	35.0	39.0	32.0	39.0
11	36.27336732873162	38.0	35.0	39.0	31.0	39.0
12	35.27826537766082	37.0	35.0	39.0	30.0	39.0
13	34.81245793894878	37.0	33.0	39.0	27.0	39.0
14	35.71818393201108	38.0	34.0	40.0	27.0	41.0
15	36.129961799082	38.0	34.0	40.0	30.0	41.0
16	36.32007072578934	38.0	35.0	40.0	31.0	41.0
17	36.276923638907896	37.0	35.0	40.0	31.0	41.0
18	36.18980931855126	37.0	35.0	40.0	31.0	41.0
19	36.08903946959873	37.0	35.0	40.0	30.0	41.0
20	35.93055132115606	36.0	34.0	40.0	30.0	41.0
21	35.77667847303105	36.0	34.0	40.0	30.0	41.0
22	35.66326396567397	36.0	34.0	40.0	30.0	41.0
23	35.64122046242921	36.0	34.0	40.0	30.0	41.0
24	35.548430797891996	36.0	34.0	40.0	30.0	41.0
25	35.455153260284675	35.0	34.0	40.0	30.0	41.0
26	35.25996082965818	35.0	34.0	40.0	29.0	41.0
27	35.17916989222536	35.0	34.0	40.0	29.0	41.0
28	35.21172848265504	36.0	34.0	40.0	29.0	41.0
29	35.229361107559676	36.0	34.0	40.0	29.0	41.0
30	35.147899651990905	36.0	34.0	40.0	29.0	41.0
31	34.9561669404001	36.0	34.0	40.0	28.0	41.0
32	34.74321473580391	35.0	34.0	40.0	27.0	41.0
33	34.512210525132666	35.0	33.0	40.0	26.0	41.0
34	34.373946102846034	35.0	33.0	40.0	25.0	41.0
35	34.18848092693776	35.0	33.0	40.0	24.0	41.0
36	33.964034727851825	35.0	33.0	40.0	23.0	41.0
37	33.8735699242585	35.0	33.0	40.0	23.0	41.0
38	33.85908230689148	35.0	33.0	40.0	23.0	41.0
39	33.82507906423908	35.0	33.0	40.0	23.0	41.0
40	33.664883886911795	35.0	33.0	40.0	23.0	41.0
41	33.64498751691223	35.0	33.0	40.0	23.0	41.0
42	33.564648626017366	35.0	33.0	39.0	23.0	41.0
43	33.48970619433716	35.0	33.0	39.0	23.0	41.0
44	33.36386479769249	35.0	32.0	39.0	23.0	41.0
45	33.29447582973546	35.0	32.0	39.0	23.0	41.0
46	33.24926941973663	35.0	32.0	39.0	23.0	41.0
47	33.16932145953077	35.0	32.0	39.0	23.0	41.0
48	33.12933518600995	35.0	32.0	39.0	23.0	41.0
49	33.10945989044106	35.0	32.0	39.0	22.0	41.0
50	33.00771043170269	35.0	32.0	39.0	22.0	40.0
51	32.83365038643481	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	26.0
15	115.0
16	403.0
17	1025.0
18	2351.0
19	4503.0
20	7610.0
21	11619.0
22	17000.0
23	24195.0
24	32977.0
25	44697.0
26	55616.0
27	64756.0
28	70672.0
29	80953.0
30	95998.0
31	116134.0
32	142781.0
33	182392.0
34	294899.0
35	426773.0
36	201439.0
37	229847.0
38	303525.0
39	434442.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.06941776967896	25.050894750078328	28.33299145923573	14.54669602100699
2	30.12754947925082	26.81064483542977	30.213954645015267	12.847851040304144
3	28.071738766977212	25.718743458145475	32.9340314121414	13.275486362735911
4	24.491895476443702	28.732035804052753	31.91905170681814	14.857017012685404
5	22.056147903164398	33.163321217877304	30.581141475463042	14.199389403495264
6	19.89946091606335	41.99909239451896	27.330410543959154	10.771036145458531
7	86.58099676437241	4.719759245703977	7.1848002776205	1.5144437123031123
8	87.55498670203424	3.7444345940994403	6.756989334933117	1.9435893689331982
9	83.27498057639973	5.510296264346419	8.646768657544717	2.5679545017091363
10	50.75752041058611	25.845857399162362	13.031374207425786	10.365247982825744
11	44.94543829898435	20.286176718935938	21.8380977937881	12.930287188291608
12	40.705719459988785	20.96122585748346	24.631443331558398	13.701611350969353
13	20.25691838434985	42.032284622830915	24.518660003400008	13.19213698941923
14	14.654632230110304	43.31564720279082	27.955478157757568	14.074242409341311
15	13.168006766297207	22.81904229357244	49.20486173066035	14.808089209470007
16	14.673353349359267	18.853010060933205	46.96573859557184	19.50789799413569
17	15.174643806997553	18.74103458595066	29.80135241646447	36.282969190587316
18	19.831741745496746	22.53313251742504	36.9394728301415	20.69565290693672
19	28.659258770124325	24.136580575275758	26.579388082830942	20.624772571768972
20	30.44208535706408	23.599885074104723	25.743014177471995	20.215015391359202
21	20.841944579867175	29.72249892169163	29.099819743369633	20.33573675507156
22	22.467029053210126	25.403786091718732	25.208777359879626	26.92040749519152
23	18.0508469813688	31.54059005595964	25.369996754537677	25.03856620813389
24	17.94779301537169	24.516798428690446	40.927633214988695	16.607775340949164
25	16.16830321328869	25.985089137816942	37.67595393410447	20.170653714789896
26	15.95924486100008	34.70635590779515	28.72704818879318	20.60735104241159
27	16.64876510861059	34.56550846278888	29.156404589730005	19.629321838870524
28	14.477571888395435	29.24828910746976	38.61158840793916	17.662550596195643
29	14.809775163923947	24.420804396969213	38.275802479196024	22.493617959910814
30	17.19518997194291	31.786493537877075	31.426928626523154	19.591387863656866
31	27.909465650785442	27.719444534205905	25.24344479833877	19.127645016669874
32	29.655798348607615	26.00244041907208	26.935616209328106	17.406145022992202
33	27.597493828704216	27.77711822615112	24.8435926003459	19.781795344798763
34	19.38475307089579	27.515444045279118	28.40120236651807	24.698600517307025
35	18.916198228904847	25.64442096596761	32.7946240532312	22.64475675189635
36	30.753038581662718	24.628071422650518	26.000578844362522	18.618311151324246
37	19.815373937673073	33.27589380172895	28.764841667802344	18.143890592795636
38	20.03194883690217	33.00940762585299	26.010448702728294	20.948194834516546
39	20.19436244929843	33.006105965047354	26.593367455178196	20.206164130476015
40	24.512934783066836	26.401028151224494	25.654325948384503	23.431711117324166
41	17.517242396696652	24.688660410839	27.527034982149956	30.26706221031439
42	20.839450772237388	25.887549647846264	25.379058759727606	27.893940820188746
43	21.06888107417778	26.239598012259695	26.6173220580446	26.074198855517917
44	19.230453114309118	29.303328495580693	29.80068505949312	21.665533330617073
45	16.53587640829883	37.09461576395513	24.111958615438002	22.257549212308028
46	21.124903935720177	33.028163869153076	26.376300819233368	19.47063137589338
47	21.0464368055097	27.53982013675901	27.68828950085914	23.72545355687216
48	22.175042816218323	24.038057611873615	32.05385781503113	21.73304175687694
49	20.897545952796083	24.135035117026312	32.635968714305186	22.331450215872415
50	19.747163030390734	33.75105196533116	26.661753982716156	19.840031021561952
51	17.85650560650104	33.9953397408969	24.850266170059417	23.297888482542646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5058.0
1	5139.0
2	5220.0
3	15639.5
4	26059.0
5	18895.0
6	11731.0
7	12944.0
8	14157.0
9	15786.0
10	17415.0
11	18021.0
12	18627.0
13	18727.0
14	18827.0
15	18399.0
16	17971.0
17	17464.0
18	16957.0
19	16087.5
20	15218.0
21	15231.0
22	15244.0
23	15698.5
24	16153.0
25	16839.5
26	19229.0
27	20932.0
28	26039.5
29	31147.0
30	33183.0
31	35219.0
32	47532.5
33	59846.0
34	67065.5
35	74285.0
36	78583.0
37	82881.0
38	98991.5
39	115102.0
40	163815.0
41	212528.0
42	257313.5
43	302099.0
44	301692.5
45	301286.0
46	288304.0
47	275322.0
48	254258.0
49	233194.0
50	213239.5
51	193285.0
52	171839.0
53	150393.0
54	137654.5
55	124916.0
56	114176.0
57	103436.0
58	93048.5
59	82661.0
60	73724.0
61	64787.0
62	59319.5
63	53852.0
64	43848.5
65	33845.0
66	29106.0
67	24367.0
68	21627.5
69	18888.0
70	16533.5
71	14179.0
72	11872.0
73	9565.0
74	7290.0
75	4122.0
76	3229.0
77	2870.5
78	2512.0
79	1740.0
80	968.0
81	853.0
82	738.0
83	510.0
84	282.0
85	171.5
86	61.0
87	54.0
88	47.0
89	30.5
90	14.0
91	9.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2847052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.859749689136297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57424305021136	14.390358122684049
2	7.788914633625222	2.7821613141319443
3	2.8044820506503494	1.5026204229687272
4	1.4501354477551343	1.0359622444900112
5	0.8784668347211935	0.7844598889164192
6	0.6005287133552994	0.6435175500998837
7	0.4474167338949829	0.5593525611866708
8	0.3516489011021683	0.5024289081715656
9	0.2759054382898369	0.44348418591551353
>10	2.5087198354441473	10.101985947369085
>50	0.8043493298316147	10.535766058800682
>100	1.4960319237533906	53.30467454174058
>500	0.016787155938628066	1.9444551290929288
>1k	0.001974959522191537	0.6014629648138412
>5k	1.974959522191537E-4	0.28993816389689037
>10k+	1.974959522191537E-4	0.577371995721192
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16369	0.5749455928448093	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8220	0.2887197002372981	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4265	0.1498040780428317	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042324481604129466	0.0
2	0.0	0.0	0.0	0.1597793085619792	0.0
3	0.0	0.0	0.0	0.23424229694434806	0.0
4	0.0	0.0	0.0	0.3584058176668357	0.0
5	0.0	0.0	0.0	0.5775798966790913	0.0
6	0.0	0.0	0.0	0.9223575825099085	0.0
7	0.0	0.0	0.0	1.0828744961454866	0.0
8	0.0	0.0	0.0	1.521890011141349	0.0
9	0.0	0.0	0.0	1.7401508648243869	0.0
10	3.512405112375889E-5	0.0	0.0	2.1089182775727315	0.0
11	3.512405112375889E-5	0.0	0.0	2.429425244077031	0.0
12	3.512405112375889E-5	0.0	0.0	2.696930017435579	0.0
13	3.512405112375889E-5	0.0	0.0	2.8243249508614525	0.0
14	3.512405112375889E-5	0.0	0.0	2.880207316199353	0.0
15	3.512405112375889E-5	0.0	0.0	2.945011190522688	0.0
16	3.512405112375889E-5	0.0	0.0	3.080344159502531	0.0
17	3.512405112375889E-5	0.0	0.0	3.239877599706644	0.0
18	3.512405112375889E-5	0.0	0.0	3.489714975349941	0.0
19	7.024810224751778E-5	0.0	0.0	3.591574723608842	0.0
20	1.0537215337127668E-4	0.0	0.0	3.7008456466548556	0.0
21	1.0537215337127668E-4	0.0	0.0	3.8342819168740156	0.0
22	1.0537215337127668E-4	0.0	0.0	3.981381442980318	0.0
23	1.0537215337127668E-4	0.0	0.0	4.14857192632941	0.0
24	1.0537215337127668E-4	0.0	0.0	4.275791239499665	0.0
25	1.0537215337127668E-4	0.0	0.0	4.3773348712984514	0.0
26	1.0537215337127668E-4	0.0	0.0	4.483444629743327	0.0
27	1.0537215337127668E-4	0.0	0.0	4.588992403370223	0.0
28	1.0537215337127668E-4	0.0	0.0	4.6993170479499495	0.0
29	1.0537215337127668E-4	0.0	0.0	4.813961950817899	0.0
30	1.0537215337127668E-4	0.0	0.0	4.98824749249399	0.0
31	1.0537215337127668E-4	0.0	0.0	5.124915175416536	0.0
32	1.0537215337127668E-4	0.0	0.0	5.2431076074479845	0.0
33	1.0537215337127668E-4	0.0	0.0	5.365304181307542	0.0
34	1.0537215337127668E-4	0.0	0.0	5.49199663371094	0.0
35	1.0537215337127668E-4	0.0	0.0	5.66673878805164	0.0
36	1.0537215337127668E-4	0.0	0.0	5.802914734258454	0.0
37	1.0537215337127668E-4	0.0	0.0	5.9422869691175295	0.0
38	1.0537215337127668E-4	0.0	0.0	6.074774889956347	0.0
39	1.0537215337127668E-4	0.0	0.0	6.212004557696874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTA	20	7.035044E-4	45.000004	12
ACATTCG	20	7.035044E-4	45.000004	22
CCGTCTA	20	7.035044E-4	45.000004	35
CTCGAAT	20	7.035044E-4	45.000004	37
ATCGATC	20	7.035044E-4	45.000004	18
TCGTTAA	35	1.2127748E-7	45.0	35
CGTAAGT	30	2.1666365E-6	44.999996	20
GCGTAAG	190	0.0	43.81579	1
CGTAAGG	485	0.0	43.144333	2
CGTTTTT	12465	0.0	42.220215	1
TGTCACG	80	0.0	42.187504	1
CGACGGT	370	0.0	41.959457	28
CGGTCTA	375	0.0	41.4	31
CGTTAGG	305	0.0	41.311478	2
AGCTACG	270	0.0	40.833332	9
TATTGCG	155	0.0	40.64516	1
ACGTAAG	140	0.0	40.17857	1
AAGGGAC	1965	0.0	40.076336	5
GTCGACG	90	0.0	40.0	1
ATCGCAA	45	1.9299478E-8	40.0	10
>>END_MODULE