FastQCFastQC Report
Thu 26 May 2016
SRR1545518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545518_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1238206
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.42480814985551674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36920.29817332495562127No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14210.11476281006553028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.033497E-445.00000418
CGGCACT207.033497E-445.00000428
TAACGCG207.033497E-445.0000041
ACTAGTC207.033497E-445.00000433
TTGACGT207.033497E-445.00000425
AACGCAC207.033497E-445.00000440
ACGTCCG207.033497E-445.00000423
CAGTCGA207.033497E-445.00000439
TACACCG207.033497E-445.00000419
AAATGCG502.1827873E-1145.01
CCTTCGT302.1656397E-644.99999628
ACACGAT302.1656397E-644.99999633
CCACGAA302.1656397E-644.99999619
CGGTCTA1450.043.44827331
CGCATCG900.042.50000421
GTTGATC6400.041.8359416
CTACGAA2050.041.70731411
CGAATAT2050.041.70731414
ATCCCGT650.041.53846419
CGTTTTT43850.041.3568951