##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545518_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1238206
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06559490101001	31.0	31.0	33.0	30.0	34.0
2	31.32304398460353	31.0	31.0	34.0	28.0	34.0
3	31.438411702091575	31.0	31.0	34.0	30.0	34.0
4	35.20217395166878	37.0	35.0	37.0	32.0	37.0
5	35.052952416641496	37.0	35.0	37.0	32.0	37.0
6	35.10345209117061	37.0	35.0	37.0	32.0	37.0
7	35.72350965832826	37.0	35.0	37.0	35.0	37.0
8	35.72458298538369	37.0	35.0	37.0	35.0	37.0
9	37.38107390854187	39.0	37.0	39.0	35.0	39.0
10	36.630148779766856	39.0	35.0	39.0	32.0	39.0
11	36.242266634146496	38.0	35.0	39.0	32.0	39.0
12	35.19133003716667	37.0	35.0	39.0	30.0	39.0
13	34.62877178757008	37.0	33.0	39.0	27.0	39.0
14	35.52728463599757	37.0	33.0	40.0	27.0	41.0
15	35.93477256611582	37.0	34.0	40.0	30.0	41.0
16	36.16927635627674	37.0	34.0	40.0	31.0	41.0
17	36.121152699954614	37.0	34.0	40.0	31.0	41.0
18	36.06292571672242	37.0	35.0	40.0	30.0	41.0
19	35.98563970777076	37.0	34.0	40.0	30.0	41.0
20	35.82572528319197	36.0	34.0	40.0	30.0	41.0
21	35.64284618229923	36.0	34.0	40.0	30.0	41.0
22	35.51818033509771	36.0	34.0	40.0	29.0	41.0
23	35.51557737565478	36.0	34.0	40.0	30.0	41.0
24	35.419307449648926	35.0	34.0	40.0	29.0	41.0
25	35.30489272382786	35.0	34.0	40.0	29.0	41.0
26	35.13330899704896	35.0	34.0	40.0	29.0	41.0
27	35.01252618708034	35.0	34.0	40.0	29.0	41.0
28	35.04412593704117	36.0	34.0	40.0	29.0	41.0
29	35.074495681655556	36.0	34.0	40.0	29.0	41.0
30	35.017143350944835	36.0	34.0	40.0	29.0	41.0
31	34.82975853775543	35.0	34.0	40.0	27.0	41.0
32	34.64125517078741	35.0	33.0	40.0	27.0	41.0
33	34.45234476331079	35.0	33.0	40.0	26.0	41.0
34	34.31887262701037	35.0	33.0	40.0	25.0	41.0
35	34.148345267265704	35.0	33.0	40.0	24.0	41.0
36	33.92679893329543	35.0	33.0	40.0	23.0	41.0
37	33.83861732215802	35.0	33.0	40.0	23.0	41.0
38	33.81730745934037	35.0	33.0	39.0	23.0	41.0
39	33.7801294776475	35.0	33.0	39.0	23.0	41.0
40	33.62302234038601	35.0	33.0	39.0	23.0	41.0
41	33.60583780081828	35.0	33.0	39.0	23.0	41.0
42	33.55235881589978	35.0	33.0	39.0	23.0	41.0
43	33.45396646438476	35.0	32.0	39.0	23.0	41.0
44	33.351158854019445	35.0	32.0	39.0	23.0	41.0
45	33.268758994868385	35.0	32.0	39.0	23.0	41.0
46	33.19667728956248	35.0	32.0	39.0	23.0	41.0
47	33.12087164817486	35.0	32.0	39.0	23.0	40.0
48	33.0856545679798	35.0	32.0	38.0	23.0	40.0
49	33.07790141543491	35.0	32.0	38.0	23.0	40.0
50	32.968449514862634	35.0	32.0	38.0	23.0	40.0
51	32.802263920543105	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	16.0
15	50.0
16	174.0
17	456.0
18	1004.0
19	1893.0
20	3301.0
21	5036.0
22	7191.0
23	10112.0
24	13921.0
25	18918.0
26	24235.0
27	28693.0
28	32079.0
29	36638.0
30	43172.0
31	52243.0
32	63886.0
33	81757.0
34	134105.0
35	186899.0
36	86848.0
37	99446.0
38	129822.0
39	176193.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.751088268026486	22.85710132239708	28.5512265325802	14.840583876996236
2	29.661865634635916	26.760490580727275	30.107187333933126	13.47045645070368
3	28.52950155305337	25.77018686712873	31.97916986349606	13.721141716321839
4	25.920081149663304	28.882108469834584	29.978371934879977	15.219438445622133
5	23.153497883227832	33.19140756869212	29.67793727376543	13.977157274314614
6	21.711734557900705	41.396019725312264	27.154286120403228	9.737959596383801
7	86.86624035095937	4.954345238191384	6.418318115079397	1.7610962957698477
8	87.96460362815233	3.914695939124831	6.077906261155252	2.042794171567574
9	82.68607969917768	5.793947049198598	8.257268984320865	3.2627042673028557
10	52.966549992489135	22.678375003836194	13.166952833373447	11.188122170301225
11	48.59821386748247	20.384653280633433	18.151987633721692	12.865145218162406
12	43.59419999580037	19.69292670201889	21.92946892520308	14.78340437697766
13	22.325525801037955	40.910236261171406	22.334086573639606	14.430151364151037
14	16.236393621093743	43.15033201260533	25.507468062664856	15.105806303636065
15	14.787119429238754	22.725216967128247	48.06155033976576	14.42611326386724
16	16.413746985558138	17.34404452893945	46.88541325110685	19.35679523439557
17	17.071634283794456	18.101592142179896	27.921282888307765	36.905490685717886
18	21.28708793205654	22.00918102480524	35.2983267727664	21.40540427037181
19	31.064620911221557	23.231675504722155	24.465395903428025	21.238307680628264
20	32.705381818534235	22.349835164746416	24.24370419784753	20.701078818871817
21	22.98042490506426	28.545573192182882	27.104859772929547	21.36914212982331
22	23.616506461768076	24.8846314748919	23.44294891157045	28.055913151769573
23	20.441913542657684	30.96334535610391	23.191859835923907	25.402881265314498
24	20.384734042639106	22.997304164250536	39.05198327257338	17.56597852053697
25	18.172016611129326	24.090337149068894	36.241626999061545	21.49601924074023
26	17.92650011387443	34.293082088117814	25.81210234807455	21.96831544993321
27	18.66571475182643	34.30883067922462	27.147017539892392	19.878437029056553
28	16.355033007431718	28.923539378746348	37.12734391530973	17.594083698512204
29	17.120010725194355	24.163749812228335	36.793069973816955	21.92316948876035
30	19.26254597377173	31.208296519319077	29.65282029000021	19.87633721690898
31	30.633109514894937	26.15130277191356	23.488337158760338	19.72725055443117
32	31.78017228151051	25.399731547093136	25.457718667168468	17.36237750422789
33	30.282521648255624	26.260250717570422	23.246777999783557	20.2104496343904
34	20.606506510225277	27.432349705945537	26.58854827064317	25.372595513186013
35	21.709634745753128	24.34909861525465	30.890417264978527	23.050849374013694
36	32.74293615117355	23.92735942161482	24.118926899078183	19.210777528133445
37	21.64340990109885	33.09287792176746	27.73052303090116	17.533189146232534
38	22.066360524823818	33.98691332460027	21.961127631428052	21.985598519147864
39	21.203499256181928	32.36731206277469	25.729079006239676	20.700109674803706
40	25.24523383023503	25.649932240677238	24.69524457158179	24.40958935750594
41	18.528903914211366	23.264141831003887	27.190952070980114	31.016002183804638
42	22.11425239418966	25.20784102160707	24.00876752333618	28.669139060867092
43	22.46685931097087	25.44431217422626	25.44608893835113	26.642739576451735
44	20.6064257482196	28.897049440884636	27.647741975083306	22.848782835812457
45	18.528903914211366	36.870036165226146	22.496256681036918	22.104803239525573
46	22.475743131595227	33.04070566610079	24.643233839926474	19.840317362377505
47	22.237899024879543	27.423708171338212	25.928238112236574	24.41015469154567
48	23.333677917890885	23.98292368151988	30.438150033193185	22.245248367396055
49	22.015641985259318	23.902807771889332	32.109358216645695	21.972192026205654
50	20.386753092781007	33.28702978341245	25.29700227587332	21.02921484793322
51	19.017352524539536	34.23743706620708	23.110855544230926	23.634354865022463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1215.0
1	1573.0
2	1931.0
3	5555.5
4	9180.0
5	6893.5
6	4607.0
7	5042.0
8	5477.0
9	5785.5
10	6094.0
11	6204.5
12	6315.0
13	6262.0
14	6209.0
15	5919.0
16	5629.0
17	5365.5
18	5102.0
19	4835.0
20	4568.0
21	4557.5
22	4547.0
23	4461.0
24	4375.0
25	5064.5
26	6821.0
27	7888.0
28	9466.0
29	11044.0
30	12173.0
31	13302.0
32	16861.5
33	20421.0
34	22604.5
35	24788.0
36	25638.0
37	26488.0
38	32900.5
39	39313.0
40	61986.0
41	84659.0
42	104514.5
43	124370.0
44	126322.0
45	128274.0
46	121972.0
47	115670.0
48	106624.0
49	97578.0
50	90345.0
51	83112.0
52	79045.5
53	74979.0
54	67576.5
55	60174.0
56	56137.5
57	52101.0
58	48642.5
59	45184.0
60	44402.0
61	43620.0
62	38282.0
63	32944.0
64	27758.5
65	22573.0
66	19978.5
67	17384.0
68	15032.0
69	12680.0
70	11331.5
71	9983.0
72	8547.0
73	7111.0
74	5524.5
75	3675.0
76	3412.0
77	2749.5
78	2087.0
79	1549.5
80	1012.0
81	817.5
82	623.0
83	429.5
84	236.0
85	175.5
86	115.0
87	103.5
88	92.0
89	64.5
90	37.0
91	22.5
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1238206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.021482313697593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09729806760252	15.235693385678658
2	6.7126285000903385	2.5536828858578153
3	2.454980355564971	1.4009209624156238
4	1.260679564899396	0.9591997618789535
5	0.766163600312746	0.7286783686373886
6	0.5086582002401275	0.5805259775750901
7	0.3904614021158639	0.5199008258169928
8	0.31181677655769685	0.47449738403251396
9	0.24963800397321023	0.4273636389662869
>10	4.133962176870484	20.39172925678245
>50	2.0915411686180883	28.79830306526707
>100	1.0187706118296316	26.808445367026152
>500	0.00212598207810858	0.28210476955391134
>1k	8.50392831243432E-4	0.4135325936723477
>5k	4.25196415621716E-4	0.4254217568387539
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5260	0.42480814985551674	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3692	0.29817332495562127	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1421	0.11476281006553028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031497182213621966	0.0
2	0.0	0.0	0.0	0.10660584749225896	0.0
3	0.0	0.0	0.0	0.14989428253457018	0.0
4	0.0	0.0	0.0	0.23929782281785097	0.0
5	0.0	0.0	0.0	0.3853962910856513	0.0
6	0.0	0.0	0.0	0.6335779345278573	0.0
7	0.0	0.0	0.0	0.7652200037796618	0.0
8	0.0	0.0	0.0	1.1662841239664483	0.0
9	0.0	0.0	0.0	1.3462218726124733	0.0
10	0.0	0.0	0.0	1.640195573272945	0.0
11	0.0	0.0	0.0	1.8416160154287735	0.0
12	0.0	0.0	0.0	2.0345564469886273	0.0
13	0.0	0.0	0.0	2.1162875967326924	0.0
14	0.0	0.0	0.0	2.1459272528157673	0.0
15	0.0	0.0	0.0	2.184854539551577	0.0
16	0.0	0.0	0.0	2.2701392175453843	0.0
17	0.0	0.0	0.0	2.3641461921521945	0.0
18	0.0	0.0	0.0	2.523166581328147	0.0
19	8.076200567595376E-5	0.0	0.0	2.574369692926702	0.0
20	8.076200567595376E-5	0.0	0.0	2.6430173977512625	0.0
21	8.076200567595376E-5	0.0	0.0	2.7220834013080215	0.0
22	8.076200567595376E-5	0.0	0.0	2.8021993109385677	0.0
23	8.076200567595376E-5	0.0	0.0	2.8964485715624058	0.0
24	8.076200567595376E-5	0.0	0.0	2.9706688547786073	0.0
25	8.076200567595376E-5	0.0	0.0	3.030109690956109	0.0
26	8.076200567595376E-5	0.0	0.0	3.0871276669633323	0.0
27	8.076200567595376E-5	0.0	0.0	3.1501220313905764	0.0
28	8.076200567595376E-5	0.0	0.0	3.2095628675680783	0.0
29	8.076200567595376E-5	0.0	0.0	3.2789374304437224	0.0
30	8.076200567595376E-5	0.0	0.0	3.38118212962948	0.0
31	1.6152401135190753E-4	0.0	0.0	3.460571181208943	0.0
32	1.6152401135190753E-4	0.0	0.0	3.5360028945102835	0.0
33	1.6152401135190753E-4	0.0	0.0	3.6079618415675583	0.0
34	1.6152401135190753E-4	0.0	0.0	3.685008794982418	0.0
35	1.6152401135190753E-4	0.0	0.0	3.792179976514409	0.0
36	2.4228601702786128E-4	0.0	0.0	3.8727804581790104	0.0
37	2.4228601702786128E-4	0.0	0.0	3.957095992104706	0.0
38	2.4228601702786128E-4	0.0	0.0	4.033416087468483	0.0
39	2.4228601702786128E-4	0.0	0.0	4.115227999218224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	20	7.033497E-4	45.000004	18
CGGCACT	20	7.033497E-4	45.000004	28
TAACGCG	20	7.033497E-4	45.000004	1
ACTAGTC	20	7.033497E-4	45.000004	33
TTGACGT	20	7.033497E-4	45.000004	25
AACGCAC	20	7.033497E-4	45.000004	40
ACGTCCG	20	7.033497E-4	45.000004	23
CAGTCGA	20	7.033497E-4	45.000004	39
TACACCG	20	7.033497E-4	45.000004	19
AAATGCG	50	2.1827873E-11	45.0	1
CCTTCGT	30	2.1656397E-6	44.999996	28
ACACGAT	30	2.1656397E-6	44.999996	33
CCACGAA	30	2.1656397E-6	44.999996	19
CGGTCTA	145	0.0	43.448273	31
CGCATCG	90	0.0	42.500004	21
GTTGATC	640	0.0	41.83594	16
CTACGAA	205	0.0	41.707314	11
CGAATAT	205	0.0	41.707314	14
ATCCCGT	65	0.0	41.538464	19
CGTTTTT	4385	0.0	41.356895	1
>>END_MODULE