FastQCFastQC Report
Thu 26 May 2016
SRR1545517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545517_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2127087
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110470.5193487619453271No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC95820.45047522738844253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27480.12919076652718015No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC207.0346403E-445.00000419
TACGGCA207.0346403E-445.00000416
CGTACTC207.0346403E-445.00000419
CCACGCA406.8193913E-945.0000041
CACGCTT253.8919286E-545.035
CGTAAGT253.8919286E-545.021
TCGCGCG351.2125747E-745.01
CCCGCAA253.8919286E-545.040
TACCGCA253.8919286E-545.031
GACCGTT302.1663764E-644.9999969
ACTTACG302.1663764E-644.9999961
ATGTACG600.044.9999961
CCGTAAG650.044.9999961
ATGCGTA302.1663764E-644.99999625
ACGCGGT302.1663764E-644.99999628
TACGTAG1400.043.3928571
CGGTCTA3200.042.8906331
CGTTTTT97950.042.7947921
ATCGTAG1000.042.751
GCGTAAG1000.042.751
Produced by FastQC (version 0.11.5)