##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545517_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2127087
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195556646249074	31.0	31.0	33.0	30.0	34.0
2	31.470689727312518	31.0	31.0	34.0	30.0	34.0
3	31.538320247361767	31.0	31.0	34.0	30.0	34.0
4	35.304156341513064	37.0	35.0	37.0	33.0	37.0
5	35.18054973773992	37.0	35.0	37.0	32.0	37.0
6	35.232798658446974	37.0	35.0	37.0	32.0	37.0
7	35.83948611410817	37.0	35.0	37.0	35.0	37.0
8	35.85982895857104	37.0	35.0	37.0	35.0	37.0
9	37.57207815195147	39.0	37.0	39.0	35.0	39.0
10	36.78572667690602	39.0	37.0	39.0	32.0	39.0
11	36.41288673194843	38.0	35.0	39.0	32.0	39.0
12	35.171061644399124	35.0	35.0	39.0	30.0	39.0
13	34.58183468753276	35.0	33.0	39.0	27.0	39.0
14	35.44475096693271	37.0	33.0	40.0	27.0	41.0
15	35.914819657117924	37.0	34.0	40.0	30.0	41.0
16	36.19160852376983	36.0	34.0	40.0	31.0	41.0
17	36.165499577591326	36.0	34.0	40.0	31.0	41.0
18	36.095230707535706	36.0	35.0	40.0	31.0	41.0
19	35.989941173069084	36.0	35.0	40.0	31.0	41.0
20	35.802820006892055	36.0	34.0	40.0	31.0	41.0
21	35.626527734878735	35.0	34.0	40.0	30.0	41.0
22	35.515564243493564	35.0	34.0	40.0	30.0	41.0
23	35.536550221029984	35.0	34.0	40.0	30.0	41.0
24	35.431687091313144	35.0	34.0	40.0	30.0	41.0
25	35.33843373590267	35.0	34.0	40.0	30.0	41.0
26	35.176176620890445	35.0	34.0	40.0	29.0	41.0
27	35.08594006733152	35.0	34.0	40.0	29.0	41.0
28	35.13862197455958	35.0	34.0	40.0	29.0	41.0
29	35.2034810987985	36.0	34.0	40.0	30.0	41.0
30	35.14879645261336	36.0	34.0	40.0	29.0	41.0
31	34.915858166591214	35.0	34.0	40.0	29.0	41.0
32	34.67630567061902	35.0	34.0	40.0	28.0	41.0
33	34.43327846956895	35.0	33.0	40.0	27.0	41.0
34	34.304970600638335	35.0	34.0	40.0	26.0	41.0
35	34.11031283628737	35.0	33.0	40.0	25.0	41.0
36	33.85951820494413	35.0	33.0	39.0	23.0	41.0
37	33.762000802035836	35.0	33.0	39.0	23.0	41.0
38	33.80941024038979	35.0	33.0	39.0	23.0	41.0
39	33.79281853539606	35.0	33.0	39.0	23.0	41.0
40	33.6103600840022	35.0	33.0	39.0	23.0	41.0
41	33.64114913964497	35.0	33.0	39.0	23.0	41.0
42	33.59415811389003	35.0	33.0	39.0	23.0	41.0
43	33.497480827065374	35.0	33.0	39.0	23.0	41.0
44	33.3901537642795	35.0	33.0	39.0	23.0	41.0
45	33.30626579918922	35.0	33.0	39.0	23.0	41.0
46	33.27718330279862	35.0	33.0	39.0	23.0	41.0
47	33.22617786672571	35.0	32.0	39.0	23.0	41.0
48	33.224305352813495	35.0	33.0	39.0	23.0	41.0
49	33.23990132984687	35.0	33.0	39.0	23.0	40.0
50	33.135563331448125	35.0	32.0	39.0	23.0	40.0
51	32.95765664497973	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	2.0
12	1.0
13	4.0
14	17.0
15	70.0
16	242.0
17	632.0
18	1415.0
19	2713.0
20	4750.0
21	7796.0
22	11413.0
23	16955.0
24	23934.0
25	33058.0
26	41566.0
27	47169.0
28	50595.0
29	57390.0
30	68780.0
31	84927.0
32	106785.0
33	140657.0
34	244019.0
35	369433.0
36	138758.0
37	155023.0
38	208436.0
39	310343.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.433404933601683	24.22242249611793	30.1323829255691	15.211789644711288
2	30.96859695912767	26.72025168693147	29.090676591977665	13.22047476196319
3	27.944696197193625	24.93748492656859	33.243163067613125	13.87465580862466
4	24.42951322630433	27.85292750131988	33.011296670046875	14.706262602328913
5	21.51792568898216	32.93673460464946	32.32994231077525	13.21539739559313
6	19.835389901776466	41.58546406423433	28.272703467230066	10.30644256675914
7	86.6346322458837	4.907368621969859	7.09021304723314	1.3677860849133112
8	87.91003847045278	3.5910143778792314	6.6525252610730075	1.846421890594978
9	83.62737396260708	5.299219072844693	8.749148483348353	2.324258481199876
10	55.2082730983735	20.160341349460552	14.94113780959594	9.690247742570003
11	52.874094947691376	19.101757473953814	18.135882547352317	9.888265031002492
12	46.54910683013906	20.498550364888697	21.466352810204754	11.485989994767491
13	20.12320135471657	48.14575050291784	21.389816213441197	10.341231928924392
14	13.710440616674353	49.39229095942009	25.646388699662964	11.25087972424259
15	11.50987242176742	23.280335971213212	53.67866946674019	11.531122140279171
16	12.54546711065415	17.38386817276397	52.583039621792615	17.48762509478926
17	13.55059760132049	17.78751879918405	28.925286083737994	39.73659751575747
18	19.407339709189138	22.488595906044274	38.39697200913738	19.707092375629205
19	29.460807197825005	24.22472611604509	25.94209827806761	20.372368408062293
20	32.263701484706544	22.720086202397926	25.799179817280628	19.217032495614895
21	20.70709848727391	30.654646471912056	28.530191759904504	20.108063280909523
22	21.714438572564262	26.425482361558316	24.49429666017422	27.3657824057032
23	17.200659869577503	33.52961115365756	23.71492092236942	25.554808054395515
24	18.237523900056743	24.241415607354096	42.641462243904456	14.879598248684703
25	14.420002566890776	25.495995227275614	40.438543416418796	19.645458789414818
26	14.384413989648753	37.58487546583661	28.51303214207975	19.51767840243488
27	15.815055989717392	38.99788772156475	28.30208637446423	16.884969914253624
28	13.048737545760938	30.366788006320384	41.02530832072219	15.55916612719649
29	12.747903588334658	24.737822195330985	40.977308403464455	21.5369658128699
30	15.126978821270592	35.01140291863943	32.66965573105378	17.19196252903619
31	28.708510747327214	28.320938447745675	25.480716115513847	17.489834689413268
32	30.59743207494569	26.670606326868622	27.342746206431613	15.389215391754075
33	28.945736587172977	28.936287044206466	24.447801147766874	17.670175220853686
34	18.660261663016133	28.674849688799753	27.942486602569616	24.722402045614494
35	18.17617238975181	26.788231981108435	32.3695739760527	22.66602165308706
36	32.43097249900921	23.983739264073357	26.40352745327295	17.18176078364449
37	18.37997223432798	34.80073922693336	30.120159636159688	16.699128902578973
38	19.356472020185354	35.69680036594648	23.442764682403684	21.50396293146449
39	19.048821228280744	35.33832889768966	26.795377904147788	18.817471969881815
40	24.378833587906843	27.35238380000442	24.042223002632237	24.2265596094565
41	15.715624231636976	24.844964028269647	27.839387857666374	31.600023882426996
42	20.20039612860217	26.698954955768144	24.792074795248148	28.308574120381536
43	21.036469124206015	26.615178410662093	26.407429503353647	25.940922961778245
44	18.906043805448483	31.13892379578268	29.025517056895183	20.929515341873653
45	15.130457757487118	41.70374789559618	22.286018390409044	20.879775956507657
46	21.006239989243504	35.253753137506834	25.579066582608046	18.160940290641612
47	20.15013960406885	29.365935666947333	26.55246353346149	23.931461195522328
48	22.2885100609425	24.735095461539654	31.534488246131914	21.44190623138593
49	20.87568585582066	23.65493277896015	33.535722798362265	21.933658566856927
50	19.098513600995165	35.762665090802585	25.314103278333235	19.824718029869018
51	16.773690967976393	37.199982887394825	22.37656475734185	23.649761387286933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3500.0
1	3429.0
2	3358.0
3	10168.0
4	16978.0
5	12494.5
6	8011.0
7	9246.0
8	10481.0
9	11966.5
10	13452.0
11	14577.5
12	15703.0
13	15308.0
14	14913.0
15	14606.5
16	14300.0
17	13801.5
18	13303.0
19	12531.5
20	11760.0
21	11473.5
22	11187.0
23	11071.5
24	10956.0
25	11837.0
26	14987.0
27	17256.0
28	19485.0
29	21714.0
30	24382.0
31	27050.0
32	33412.0
33	39774.0
34	45582.0
35	51390.0
36	57518.5
37	63647.0
38	76804.0
39	89961.0
40	127281.5
41	164602.0
42	209955.0
43	255308.0
44	262463.0
45	269618.0
46	250917.5
47	232217.0
48	201288.0
49	170359.0
50	158093.0
51	145827.0
52	132035.5
53	118244.0
54	102600.0
55	86956.0
56	73863.0
57	60770.0
58	53301.0
59	45832.0
60	39797.0
61	33762.0
62	29962.0
63	26162.0
64	20878.0
65	15594.0
66	12896.0
67	10198.0
68	8713.0
69	7228.0
70	5987.0
71	4746.0
72	4197.0
73	3648.0
74	2921.0
75	1776.5
76	1359.0
77	859.0
78	359.0
79	413.0
80	467.0
81	277.0
82	87.0
83	54.5
84	22.0
85	63.0
86	104.0
87	56.0
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2127087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.949564395198582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57019141606638	14.461998391561599
2	7.653448700612377	2.747521405939816
3	2.7879852876860407	1.5012936436256052
4	1.4824605869200935	1.0643808707306444
5	0.8687547546204454	0.7796884705847315
6	0.581798632111351	0.6265819207272687
7	0.4443662211018306	0.5583326070502852
8	0.35765678004169027	0.5135826723790156
9	0.2908202306698366	0.46980868120512637
>10	2.6104295093288568	10.294743289851597
>50	0.8147212121891192	10.669543937446882
>100	1.5121854367489456	52.26738275378544
>500	0.023082795911148965	2.660115012599169
>1k	0.001573826993941975	0.4137611414489017
>5k	2.623044989903292E-4	0.4511446583253728
>10k+	2.623044989903292E-4	0.5201205427385089
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11047	0.5193487619453271	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9582	0.45047522738844253	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2748	0.12919076652718015	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04390981657073735	0.0
2	0.0	0.0	0.0	0.15876172436764457	0.0
3	0.0	0.0	0.0	0.221758677477696	0.0
4	0.0	0.0	0.0	0.3319093201171367	0.0
5	0.0	0.0	0.0	0.5287512922602602	0.0
6	0.0	0.0	0.0	0.8236616555881353	0.0
7	0.0	0.0	0.0	0.9863254300364771	0.0
8	0.0	0.0	0.0	1.4459211118304047	0.0
9	0.0	0.0	0.0	1.6463830581447774	0.0
10	0.0	0.0	0.0	1.9769290113662488	0.0
11	4.701265157466526E-5	0.0	0.0	2.2530813267158325	0.0
12	9.402530314933052E-5	0.0	0.0	2.4848066863273575	0.0
13	9.402530314933052E-5	0.0	0.0	2.591807481311296	0.0
14	9.402530314933052E-5	0.0	0.0	2.635200158714712	0.0
15	9.402530314933052E-5	0.0	0.0	2.689217695374002	0.0
16	9.402530314933052E-5	0.0	0.0	2.8099461846177425	0.0
17	9.402530314933052E-5	0.0	0.0	2.948774544717729	0.0
18	9.402530314933052E-5	0.0	0.0	3.158168894831288	0.0
19	9.402530314933052E-5	0.0	0.0	3.237338200083024	0.0
20	9.402530314933052E-5	0.0	0.0	3.331786617096527	0.0
21	3.7610121259732207E-4	0.0	0.0	3.453126270810738	0.0
22	3.7610121259732207E-4	0.0	0.0	3.5790261517276916	0.0
23	3.7610121259732207E-4	0.0	0.0	3.736142433290223	0.0
24	3.7610121259732207E-4	0.0	0.0	3.8440364686540796	0.0
25	3.7610121259732207E-4	0.0	0.0	3.939190075441202	0.0
26	4.231138641719873E-4	0.0	0.0	4.03561302382084	0.0
27	4.231138641719873E-4	0.0	0.0	4.134715693340235	0.0
28	4.231138641719873E-4	0.0	0.0	4.231091629068299	0.0
29	4.231138641719873E-4	0.0	0.0	4.33832748731011	0.0
30	4.231138641719873E-4	0.0	0.0	4.493422224854931	0.0
31	4.701265157466526E-4	0.0	0.0	4.624681548051396	0.0
32	4.701265157466526E-4	0.0	0.0	4.736994772663271	0.0
33	4.701265157466526E-4	0.0	0.0	4.854573414251509	0.0
34	4.701265157466526E-4	0.0	0.0	4.976054105920444	0.0
35	4.701265157466526E-4	0.0	0.0	5.136696336351076	0.0
36	4.701265157466526E-4	0.0	0.0	5.262173103403857	0.0
37	4.701265157466526E-4	0.0	0.0	5.397381489332594	0.0
38	4.701265157466526E-4	0.0	0.0	5.5257730407830055	0.0
39	4.701265157466526E-4	0.0	0.0	5.657972617010964	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATC	20	7.0346403E-4	45.000004	19
TACGGCA	20	7.0346403E-4	45.000004	16
CGTACTC	20	7.0346403E-4	45.000004	19
CCACGCA	40	6.8193913E-9	45.000004	1
CACGCTT	25	3.8919286E-5	45.0	35
CGTAAGT	25	3.8919286E-5	45.0	21
TCGCGCG	35	1.2125747E-7	45.0	1
CCCGCAA	25	3.8919286E-5	45.0	40
TACCGCA	25	3.8919286E-5	45.0	31
GACCGTT	30	2.1663764E-6	44.999996	9
ACTTACG	30	2.1663764E-6	44.999996	1
ATGTACG	60	0.0	44.999996	1
CCGTAAG	65	0.0	44.999996	1
ATGCGTA	30	2.1663764E-6	44.999996	25
ACGCGGT	30	2.1663764E-6	44.999996	28
TACGTAG	140	0.0	43.392857	1
CGGTCTA	320	0.0	42.89063	31
CGTTTTT	9795	0.0	42.794792	1
ATCGTAG	100	0.0	42.75	1
GCGTAAG	100	0.0	42.75	1
>>END_MODULE