FastQCFastQC Report
Thu 26 May 2016
SRR1545516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545516_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2368183
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144200.6089056462275086No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40860.17253734191994452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG900.045.01
CGTTATG207.034804E-445.031
CGGTCTA3250.042.92307331
GCGATAT3200.042.18759
TACGGGA6600.040.909094
CGTATAA501.0822987E-940.542
CGTTTTT95500.040.405761
CGTTCGA1350.040.00000414
GTTGATC16000.039.79687516
TGTTACG1250.039.61
CGACGGT3600.039.37528
GCGTAAT403.4603E-739.37512
AACGGGA5700.039.078954
CGCATCG2650.039.05660221
ATATGCT16100.038.85093334
GACTAAT20750.038.8192759
AATGGGC7600.038.7828944
TAGCATA16500.038.59090830
CATACCG356.251097E-638.5714261
CGTTGAT2750.038.45454425
Produced by FastQC (version 0.11.5)