##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545516_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2368183
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12835030063133	31.0	31.0	33.0	30.0	34.0
2	31.414150426719555	31.0	31.0	34.0	30.0	34.0
3	31.517980662811954	31.0	31.0	34.0	30.0	34.0
4	35.29723167508592	37.0	35.0	37.0	33.0	37.0
5	35.17337807086699	37.0	35.0	37.0	32.0	37.0
6	35.22569919638811	37.0	35.0	37.0	32.0	37.0
7	35.7749646036645	37.0	35.0	37.0	35.0	37.0
8	35.76005274930189	37.0	35.0	37.0	35.0	37.0
9	37.41792885093762	39.0	37.0	39.0	35.0	39.0
10	36.68278211607802	39.0	35.0	39.0	32.0	39.0
11	36.37338921865413	38.0	35.0	39.0	32.0	39.0
12	35.74411521406918	37.0	35.0	39.0	31.0	39.0
13	35.48260206242507	37.0	35.0	39.0	30.0	39.0
14	36.47045055217439	38.0	35.0	40.0	31.0	41.0
15	36.707076691286105	38.0	35.0	40.0	31.0	41.0
16	36.78462601918855	38.0	35.0	40.0	31.0	41.0
17	36.711289625843946	38.0	35.0	40.0	31.0	41.0
18	36.620457962919254	38.0	35.0	40.0	31.0	41.0
19	36.555004406331776	38.0	35.0	40.0	31.0	41.0
20	36.42209153600039	38.0	35.0	40.0	31.0	41.0
21	36.294416014302946	38.0	35.0	40.0	30.0	41.0
22	36.19156374317356	38.0	35.0	40.0	30.0	41.0
23	36.12305341267968	38.0	34.0	40.0	30.0	41.0
24	36.03168505136639	38.0	34.0	40.0	30.0	41.0
25	35.9185472575388	37.0	34.0	40.0	30.0	41.0
26	35.76378007949555	37.0	34.0	40.0	30.0	41.0
27	35.65054220894247	37.0	34.0	40.0	29.0	41.0
28	35.65772661994449	37.0	34.0	40.0	29.0	41.0
29	35.623648172459646	37.0	34.0	40.0	29.0	41.0
30	35.55486928163913	37.0	34.0	40.0	29.0	41.0
31	35.42608573746201	37.0	34.0	40.0	29.0	41.0
32	35.28753943424136	37.0	34.0	40.0	28.0	41.0
33	35.111308965565584	37.0	34.0	40.0	27.0	41.0
34	34.93501853530745	37.0	34.0	40.0	26.0	41.0
35	34.78806452035168	37.0	33.0	40.0	25.0	41.0
36	34.61308226602421	37.0	33.0	40.0	24.0	41.0
37	34.540573933686716	36.0	33.0	40.0	24.0	41.0
38	34.49065253825401	36.0	33.0	40.0	24.0	41.0
39	34.42570823285194	36.0	33.0	40.0	24.0	41.0
40	34.26520501160594	36.0	33.0	40.0	23.0	41.0
41	34.17279872374728	36.0	33.0	40.0	23.0	41.0
42	34.103777453009336	36.0	33.0	40.0	23.0	41.0
43	34.04752166534428	35.0	33.0	40.0	23.0	41.0
44	33.920569905281816	35.0	33.0	40.0	23.0	41.0
45	33.85446648337565	35.0	33.0	40.0	23.0	41.0
46	33.78789899260319	35.0	33.0	40.0	23.0	41.0
47	33.69481032504667	35.0	33.0	39.0	23.0	41.0
48	33.59456596048532	35.0	32.0	39.0	23.0	41.0
49	33.55051953332998	35.0	32.0	39.0	23.0	41.0
50	33.49463998348101	35.0	32.0	39.0	23.0	41.0
51	33.33894973488113	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	13.0
14	46.0
15	100.0
16	246.0
17	696.0
18	1569.0
19	2993.0
20	5006.0
21	8125.0
22	11703.0
23	16606.0
24	23080.0
25	31447.0
26	40728.0
27	47824.0
28	54428.0
29	63101.0
30	74448.0
31	89674.0
32	110532.0
33	139199.0
34	217407.0
35	289399.0
36	191016.0
37	230294.0
38	302363.0
39	415838.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81608727028274	24.364544462991248	27.896492796375956	14.922875470350053
2	32.01179976378515	25.94123849381572	29.095386631860798	12.951575110538332
3	29.39515231719846	25.833561004364952	31.472229975470643	13.299056702965945
4	26.68341086816348	27.985421734722358	30.585474179993692	14.745693217120467
5	22.932096041564357	33.11728865547975	29.346760786645287	14.603854516310605
6	21.91971650839483	41.870159527367605	26.014754771907405	10.195369192330153
7	87.2273806542822	4.219099621946446	6.936203832220736	1.6173158915506107
8	87.6662403201104	3.742067230446296	6.860365098474232	1.7313273509690763
9	82.17540620805065	6.199858710243254	8.563316263988046	3.0614188177180566
10	44.92093727553994	28.255333308278964	14.598829566802904	12.224899849378195
11	39.63979979587726	23.097370431254678	21.91296027376263	15.34986949910543
12	35.798838181002054	21.482123636560182	26.228294012751547	16.49074416968621
13	23.13866791544403	32.93199892069152	26.315660571839256	17.613672592025196
14	18.47521918703073	35.52850434278094	27.944757647529773	18.05151882265855
15	18.258935225867255	23.680433480014003	40.72768025106168	17.33295104305706
16	20.46940629165905	19.883218484382333	39.539005220458044	20.108370003500575
17	19.797329851620418	20.787456036970116	29.126591990568297	30.28862212084117
18	22.11957437410876	23.165777306905756	32.84226767948254	21.872380639502946
19	29.231947024364246	24.084836349217944	25.9963862590011	20.686830367416707
20	30.519178627665177	22.89413444822465	25.92595251296036	20.660734411149814
21	25.115499942360874	25.868989009717573	27.530600464575585	21.484910583345965
22	24.2659456638275	24.625419572727278	25.424133185653304	25.68450157779192
23	22.41444178933807	27.890581091072775	25.83482779835849	23.860149321230665
24	21.66238842184071	24.111354570149352	34.54737239478537	19.67888461322457
25	21.578399980069108	25.453818391568557	31.56669058092217	21.40109104744017
26	20.567962864356343	30.019301717814884	26.731886851649556	22.68084856617922
27	20.688730558407016	30.047382318004985	27.995851671935828	21.268035451652175
28	19.64117637868357	27.880826777322532	32.630333044363546	19.84766379963035
29	19.915986222348526	25.245895270762436	32.19417587238824	22.643942634500796
30	21.07911424074913	28.13051187344897	30.290817897096634	20.499555988705264
31	27.761874821329265	26.447744958898866	25.344282937593928	20.446097282177938
32	28.664634447591254	25.73183744668381	26.19683529524534	19.406692810479594
33	27.82056960969655	26.125345887543318	24.49101273001284	21.563071772747293
34	21.547616886026123	26.463030939754233	27.97879217948951	24.010559994730137
35	22.80102508969957	25.214563232655586	29.360188802976793	22.62422287466805
36	28.421367774365407	26.81199045850764	24.825024079642493	19.941617687484456
37	22.48483330891236	31.683488987126417	26.323556921065645	19.50812078289558
38	22.67953954571923	31.265700328057417	24.888194873453614	21.16656525276974
39	22.90363540317619	29.957862209128265	25.42649786777458	21.712004519920967
40	24.703327403329894	25.976497593302543	26.82014016653274	22.50003483683482
41	20.645195071495742	25.48113891536254	27.318116885392723	26.555549127748996
42	22.16412329621486	26.84741846386027	25.363327073963454	25.625131165961413
43	22.60264515031144	25.799273113606507	27.005894392451935	24.59218734363012
44	21.30147881308159	27.763901691718925	28.01873841675242	22.91588107844706
45	20.412316109016913	31.91826814059555	25.210889530074322	22.45852622031321
46	22.58706358419092	30.25872578259366	26.347330421677718	20.806880211537706
47	22.874330235459002	27.581356677250024	26.623744871067817	22.920568216223156
48	23.724855722720754	25.33820232642494	28.864323407439375	22.07261854341493
49	22.5057776362722	25.60249777994353	29.698422799251578	22.1933017845327
50	21.603651407006975	30.201888958750235	26.88398658380708	21.310473050435714
51	20.654231535316317	30.67288296554785	26.14379885338253	22.529086645753303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2403.0
1	3591.5
2	4780.0
3	14489.0
4	24198.0
5	17337.0
6	10476.0
7	10708.5
8	10941.0
9	11417.5
10	11894.0
11	12033.5
12	12173.0
13	11880.0
14	11587.0
15	11554.5
16	11522.0
17	10828.0
18	10134.0
19	10423.5
20	10713.0
21	10783.0
22	10853.0
23	10895.5
24	10938.0
25	12697.0
26	16454.5
27	18453.0
28	22957.0
29	27461.0
30	33721.0
31	39981.0
32	42763.5
33	45546.0
34	50649.0
35	55752.0
36	60729.0
37	65706.0
38	77898.0
39	90090.0
40	112052.0
41	134014.0
42	156080.0
43	178146.0
44	187277.0
45	196408.0
46	190604.0
47	184800.0
48	175517.5
49	166235.0
50	156315.5
51	146396.0
52	140095.5
53	133795.0
54	129188.5
55	124582.0
56	120399.0
57	116216.0
58	113046.5
59	109877.0
60	103012.5
61	96148.0
62	87878.0
63	79608.0
64	69354.5
65	59101.0
66	50454.5
67	41808.0
68	36495.0
69	31182.0
70	28237.0
71	25292.0
72	21399.0
73	17506.0
74	14829.5
75	9343.5
76	6534.0
77	5264.0
78	3994.0
79	3079.0
80	2164.0
81	1604.5
82	1045.0
83	871.0
84	697.0
85	477.0
86	257.0
87	174.0
88	91.0
89	67.5
90	44.0
91	28.5
92	13.0
93	14.5
94	16.0
95	10.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2368183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.960627400632255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66429053910001	13.511563489693886
2	6.265620915400619	2.1253772355943656
3	2.2920230741462304	1.1662244806273778
4	1.2558577740801509	0.8520054309744335
5	0.8260781311143266	0.7005401692820366
6	0.5772521319534974	0.5874334995770324
7	0.44414160090202653	0.5273044144213711
8	0.3469362804890601	0.47074055881089516
9	0.3045013435621537	0.46480804480346405
>10	4.57821627222283	19.567843834286496
>50	2.1065063884249806	25.972808615950292
>100	1.3308294515791654	32.2712621994541
>500	0.005996978341984907	0.6809229089694496
>1k	0.0014992445854962266	0.4900424221331764
>5k	0.0	0.0
>10k+	2.498740975827045E-4	0.6111226954216383
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14420	0.6089056462275086	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4086	0.17253734191994452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016088283717939026	0.0
2	0.0	0.0	0.0	0.059834902961468775	0.0
3	0.0	0.0	0.0	0.09454505838442384	0.0
4	0.0	0.0	0.0	0.1400229627524562	0.0
5	0.0	0.0	0.0	0.221604495936336	0.0
6	0.0	0.0	0.0	0.37661785427899785	0.0
7	0.0	0.0	0.0	0.4605218431176982	0.0
8	0.0	0.0	0.0	0.6791282599359931	0.0
9	0.0	0.0	0.0	0.7828786880067967	0.0
10	0.0	0.0	0.0	0.9475196807003513	0.0
11	0.0	0.0	0.0	1.067358392489094	0.0
12	0.0	0.0	0.0	1.1732623703489131	0.0
13	0.0	0.0	0.0	1.225538735815602	0.0
14	0.0	0.0	0.0	1.2506634833541157	0.0
15	0.0	0.0	0.0	1.2763794014229475	0.0
16	0.0	0.0	0.0	1.3261644053690107	0.0
17	0.0	0.0	0.0	1.380256508893105	0.0
18	0.0	0.0	0.0	1.4708745058975594	4.2226466451283535E-5
19	0.0	0.0	0.0	1.509976213831448	4.2226466451283535E-5
20	0.0	0.0	0.0	1.5550318535349674	4.2226466451283535E-5
21	0.0	0.0	0.0	1.6070126337364976	4.2226466451283535E-5
22	0.0	0.0	0.0	1.6661296867682944	4.2226466451283535E-5
23	0.0	0.0	0.0	1.732002974432297	4.2226466451283535E-5
24	0.0	0.0	0.0	1.7891353835408834	4.2226466451283535E-5
25	0.0	0.0	0.0	1.8348244202411723	4.2226466451283535E-5
26	0.0	0.0	0.0	1.8787399453505071	4.2226466451283535E-5
27	0.0	0.0	0.0	1.9285249492965704	4.2226466451283535E-5
28	0.0	0.0	0.0	1.9762408563865208	4.2226466451283535E-5
29	0.0	0.0	0.0	2.0283483159874045	4.2226466451283535E-5
30	0.0	0.0	0.0	2.1053693907945457	4.2226466451283535E-5
31	0.0	0.0	0.0	2.1687513169379224	4.2226466451283535E-5
32	0.0	0.0	0.0	2.226052631912314	4.2226466451283535E-5
33	0.0	0.0	0.0	2.2847896467460496	4.2226466451283535E-5
34	4.2226466451283535E-5	0.0	0.0	2.3422176411197952	4.2226466451283535E-5
35	4.2226466451283535E-5	0.0	0.0	2.4205899628533776	4.2226466451283535E-5
36	8.445293290256707E-5	0.0	0.0	2.487856723910272	4.2226466451283535E-5
37	8.445293290256707E-5	0.0	0.0	2.5587971875484286	4.2226466451283535E-5
38	8.445293290256707E-5	0.0	0.0	2.6269929308672513	4.2226466451283535E-5
39	8.445293290256707E-5	0.0	0.0	2.696497694646064	4.2226466451283535E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	90	0.0	45.0	1
CGTTATG	20	7.034804E-4	45.0	31
CGGTCTA	325	0.0	42.923073	31
GCGATAT	320	0.0	42.1875	9
TACGGGA	660	0.0	40.90909	4
CGTATAA	50	1.0822987E-9	40.5	42
CGTTTTT	9550	0.0	40.40576	1
CGTTCGA	135	0.0	40.000004	14
GTTGATC	1600	0.0	39.796875	16
TGTTACG	125	0.0	39.6	1
CGACGGT	360	0.0	39.375	28
GCGTAAT	40	3.4603E-7	39.375	12
AACGGGA	570	0.0	39.07895	4
CGCATCG	265	0.0	39.056602	21
ATATGCT	1610	0.0	38.850933	34
GACTAAT	2075	0.0	38.819275	9
AATGGGC	760	0.0	38.782894	4
TAGCATA	1650	0.0	38.590908	30
CATACCG	35	6.251097E-6	38.571426	1
CGTTGAT	275	0.0	38.454544	25
>>END_MODULE