FastQCFastQC Report
Thu 26 May 2016
SRR1545515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545515_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences470828
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.41055332308188974No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15400.3270833510326489No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5870.12467397860789926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA302.1627602E-645.0000046
TCTTGCG302.1627602E-645.0000041
AGTCCAT302.1627602E-645.00000438
CTAAGCG302.1627602E-645.0000041
GTAATCC453.8380676E-1045.0000049
TCGCTAT302.1627602E-645.00000423
TCGAGAG453.8380676E-1045.0000041
TAGTGCG302.1627602E-645.0000041
CAATACG302.1627602E-645.0000041
TGTCACG207.02904E-445.01
GGTACGA253.887285E-545.021
CACGGGT253.887285E-545.04
CGACGGT850.045.028
TCCGATC207.02904E-445.014
GACGTCG253.887285E-545.019
GACCGAA207.02904E-445.09
CCTACTG207.02904E-445.024
AAGCGAC253.887285E-545.015
GGATACC207.02904E-445.08
ACGGGTA502.1827873E-1145.05