##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545515_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	470828
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04568759716924	31.0	31.0	33.0	30.0	34.0
2	31.33011418182436	31.0	31.0	34.0	30.0	34.0
3	31.443934940147994	31.0	31.0	34.0	30.0	34.0
4	35.2077998759632	37.0	35.0	37.0	32.0	37.0
5	35.05769835268931	37.0	35.0	37.0	32.0	37.0
6	35.105012870942254	37.0	35.0	37.0	32.0	37.0
7	35.73076155198926	37.0	35.0	37.0	35.0	37.0
8	35.7469394343582	37.0	35.0	37.0	35.0	37.0
9	37.45648729472334	39.0	37.0	39.0	35.0	39.0
10	36.629081108175384	39.0	35.0	39.0	32.0	39.0
11	36.208362714197115	38.0	35.0	39.0	31.0	39.0
12	35.081382160789076	37.0	34.0	39.0	30.0	39.0
13	34.534452071669484	35.0	33.0	39.0	26.0	39.0
14	35.43519289422039	37.0	33.0	40.0	27.0	41.0
15	35.88911662008207	37.0	34.0	40.0	30.0	41.0
16	36.12709099713696	37.0	34.0	40.0	31.0	41.0
17	36.07801362705701	37.0	34.0	40.0	31.0	41.0
18	36.02421053973001	36.0	35.0	40.0	30.0	41.0
19	35.923086562396456	36.0	34.0	40.0	30.0	41.0
20	35.77566329954888	36.0	34.0	40.0	30.0	41.0
21	35.624198221006395	36.0	34.0	40.0	30.0	41.0
22	35.50626343378049	36.0	34.0	40.0	30.0	41.0
23	35.49280629019515	35.0	34.0	40.0	30.0	41.0
24	35.40853984894696	35.0	34.0	40.0	29.0	41.0
25	35.32728937106544	35.0	34.0	40.0	29.0	41.0
26	35.14004264827071	35.0	34.0	40.0	29.0	41.0
27	35.050154196436914	35.0	34.0	40.0	29.0	41.0
28	35.11903497667938	36.0	34.0	40.0	29.0	41.0
29	35.14676060047406	36.0	34.0	40.0	29.0	41.0
30	35.10120468621238	36.0	34.0	40.0	29.0	41.0
31	34.91156643190295	35.0	34.0	40.0	29.0	41.0
32	34.73696551606956	35.0	34.0	40.0	27.0	41.0
33	34.519130128199684	35.0	33.0	40.0	26.0	41.0
34	34.40778373418743	35.0	33.0	40.0	25.0	41.0
35	34.253100920081216	35.0	33.0	40.0	25.0	41.0
36	33.99836245932697	35.0	33.0	40.0	23.0	41.0
37	33.93799434188281	35.0	33.0	40.0	23.0	41.0
38	33.97262694657072	35.0	33.0	40.0	24.0	41.0
39	33.9373019446592	35.0	33.0	40.0	23.0	41.0
40	33.78574341373071	35.0	33.0	39.0	23.0	41.0
41	33.777681021519534	35.0	33.0	39.0	23.0	41.0
42	33.72337031782307	35.0	33.0	39.0	23.0	41.0
43	33.63268327287247	35.0	33.0	39.0	23.0	41.0
44	33.52497515016099	35.0	33.0	39.0	23.0	41.0
45	33.47777957130842	35.0	32.0	39.0	23.0	41.0
46	33.43750371685626	35.0	32.0	39.0	23.0	41.0
47	33.35234947794099	35.0	32.0	39.0	23.0	41.0
48	33.35048892589226	35.0	32.0	39.0	23.0	41.0
49	33.35913751943385	35.0	32.0	39.0	23.0	40.0
50	33.262150934099076	35.0	32.0	39.0	23.0	40.0
51	33.08955499672917	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	20.0
16	55.0
17	168.0
18	377.0
19	703.0
20	1157.0
21	1734.0
22	2715.0
23	3708.0
24	5273.0
25	7032.0
26	9001.0
27	10462.0
28	11846.0
29	13595.0
30	16049.0
31	19405.0
32	24115.0
33	30921.0
34	51087.0
35	73635.0
36	31777.0
37	36917.0
38	49037.0
39	69986.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.906556109662127	24.514684768110648	28.84960112822517	14.729157994002056
2	28.79480404733788	26.7144264996984	30.837588248787245	13.653181204176473
3	27.383885410383407	26.051764126177712	31.851121853415687	14.713228610023194
4	24.585198841190415	28.66120961370182	31.210335833892632	15.543255711215137
5	21.952390257163977	33.7335502561445	29.85782493819399	14.456234548497541
6	20.158104445784872	41.91828013627057	27.728809671472387	10.194805746472174
7	88.21947717637863	4.379731027041722	5.841623692728555	1.5591681038510878
8	89.77269831021096	3.077556984716287	5.6126653470057	1.5370793580670647
9	85.56011961905409	4.9495781899122395	7.047371864035274	2.4429303269983946
10	53.929885223478635	24.305903642094354	12.091039615315996	9.673171519111012
11	49.32926673859669	18.451324050396323	19.818914762928287	12.400494448078705
12	44.78726838675694	20.67570322920472	22.077913802917415	12.459114581120918
13	20.069537070862395	44.756896361303916	22.063471161443246	13.110095406390442
14	13.767447985251513	45.5204448333574	26.24185477499214	14.47025240639894
15	12.149022572999058	21.974691394734382	51.26224438648509	14.614041645781475
16	14.750821956213308	17.15892003024459	48.13392576482282	19.956332248719278
17	14.767813299124096	17.77676773683808	28.46708352094608	38.98833544309174
18	20.52023244157102	21.551182172683017	36.89733830613303	21.031247079612935
19	29.068789451774318	24.02129864833867	24.584349274044875	22.32556262584213
20	31.807156753634025	21.90927472452785	26.03456039148054	20.24900813035758
21	20.62600355119067	29.970392584977958	27.49454153108991	21.90906233274147
22	22.698310210947522	24.82180329122312	24.158503742343278	28.32138275548608
23	18.396526969509036	31.94521141478417	23.523452301052615	26.134809314654184
24	18.05075314127452	23.047907091336963	42.424197371439256	16.477142395949265
25	16.12266050447297	25.336003806060813	38.236468519289424	20.304867170176795
26	16.264538217778043	35.71028061202817	26.465290934268992	21.559890235924797
27	15.860781431860469	36.15375466199971	27.609870271096877	20.375593635042947
28	14.11237224634049	28.784821633377796	38.46394012250759	18.638865997774133
29	13.782315410298454	23.687418760141707	38.384293202613264	24.14597262694657
30	16.672967622996083	32.94876260545252	31.10307798176829	19.275191789783104
31	28.934557842779103	27.894900048425324	24.02342256620252	19.14711954259305
32	31.008776028613422	25.316463761713408	25.14718750796469	18.52757270170848
33	28.32350667334993	28.025733388838386	24.115388209707152	19.53537172810453
34	18.430084871757842	27.014960877432948	27.015810444578488	27.539143806230726
35	19.338059758553015	25.46152735181425	31.98514107062452	23.215271819008215
36	31.442692448197647	24.767855777481373	24.69564257011053	19.093809204210455
37	20.04383766470983	33.75776291979237	28.128955797021415	18.069443618476384
38	19.078092212017978	33.90664956204814	24.90463608791321	22.110622138020677
39	19.697214269329777	34.00010194805746	26.46847681106476	19.834206971547996
40	25.420748128828365	25.66627303388923	24.467109007960445	24.44586982932196
41	16.397283084268565	24.645730500310094	27.163210344329563	31.793776071091777
42	20.467346886761195	25.495510037635828	24.36707247657319	29.670070599029795
43	20.34500921780353	25.89586855497124	27.08377581622163	26.67534641100359
44	18.91221422685142	29.211729124011317	29.83318749097335	22.042869158163917
45	15.856321204346385	39.89737228881884	21.43882691768544	22.80747958914933
46	21.513376434706515	34.6874017687988	24.64211983994155	19.157101956553134
47	19.508822754806427	28.6937055570187	26.75117027874298	25.04630140943189
48	21.80817623420867	24.184415540282224	31.532746565624812	22.47466165988429
49	20.66168537130332	23.664480447212146	33.092976628407826	22.580857553076708
50	19.54896480243316	34.523435309709704	25.788823094633283	20.138776793223855
51	16.66532151868623	35.42164017433117	24.272345739845548	23.640692567137044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	433.0
1	505.5
2	578.0
3	1858.5
4	3139.0
5	2294.5
6	1450.0
7	1633.0
8	1816.0
9	2022.0
10	2228.0
11	2320.5
12	2413.0
13	2365.0
14	2317.0
15	2208.0
16	2099.0
17	2023.0
18	1947.0
19	1812.5
20	1678.0
21	1705.0
22	1732.0
23	1709.0
24	1686.0
25	2082.0
26	2798.0
27	3118.0
28	3462.0
29	3806.0
30	4921.5
31	6037.0
32	7872.0
33	9707.0
34	11217.0
35	12727.0
36	13601.5
37	14476.0
38	16957.5
39	19439.0
40	27957.0
41	36475.0
42	44916.5
43	53358.0
44	51318.5
45	49279.0
46	46366.5
47	43454.0
48	41226.5
49	38999.0
50	36420.5
51	33842.0
52	31494.5
53	29147.0
54	25320.0
55	21493.0
56	19714.5
57	17936.0
58	16086.5
59	14237.0
60	12541.0
61	10845.0
62	9739.5
63	8634.0
64	7157.5
65	5681.0
66	4844.5
67	4008.0
68	3243.5
69	2479.0
70	2230.5
71	1982.0
72	1773.5
73	1565.0
74	1270.0
75	765.0
76	555.0
77	382.0
78	209.0
79	197.5
80	186.0
81	145.5
82	105.0
83	86.5
84	68.0
85	39.5
86	11.0
87	5.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.691487812560812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8773708164187	16.24201576500943
2	6.606497644013046	2.8660952625764136
3	2.6633583512652788	1.7331661565085852
4	1.4879596510391018	1.2910423454438764
5	1.120066872865744	1.2147958461010193
6	0.8722603830617195	1.1352375281137745
7	0.6804219312316705	1.033155482009761
8	0.6184744476923374	1.073250475559889
9	0.5665185860788464	1.105976790496663
>10	8.860470562334282	47.19484486440012
>50	1.539691603102457	21.23830870786798
>100	0.10391169806791405	2.992184113525576
>500	9.991509429607118E-4	0.12722092384756722
>1k	0.0019983018859214235	0.7527057385393543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.41055332308188974	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1540	0.3270833510326489	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	587	0.12467397860789926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03653138725819195	0.0
2	0.0	0.0	0.0	0.1127800385703484	0.0
3	0.0	0.0	0.0	0.15738231371116415	0.0
4	0.0	0.0	0.0	0.24679925577918052	0.0
5	0.0	0.0	0.0	0.4145887670232017	0.0
6	0.0	0.0	0.0	0.6558658363563764	0.0
7	0.0	0.0	0.0	0.7858496096238966	0.0
8	0.0	0.0	0.0	1.1369332325180321	0.0
9	0.0	0.0	0.0	1.2796605129686425	0.0
10	2.1239178638483692E-4	0.0	0.0	1.5205127987290477	0.0
11	2.1239178638483692E-4	0.0	0.0	1.7129397571937097	0.0
12	2.1239178638483692E-4	0.0	0.0	1.8837027534471187	0.0
13	2.1239178638483692E-4	0.0	0.0	1.9582522704681964	0.0
14	2.1239178638483692E-4	0.0	0.0	1.9907482137850765	0.0
15	2.1239178638483692E-4	0.0	0.0	2.0272796010432685	0.0
16	2.1239178638483692E-4	0.0	0.0	2.1122363155972033	0.0
17	2.1239178638483692E-4	0.0	0.0	2.2148215484210794	0.0
18	2.1239178638483692E-4	0.0	0.0	2.374540171782477	0.0
19	2.1239178638483692E-4	0.0	0.0	2.436770965193234	0.0
20	4.2478357276967384E-4	0.0	0.0	2.5106833068551575	0.0
21	4.2478357276967384E-4	0.0	0.0	2.602436558573407	0.0
22	4.2478357276967384E-4	0.0	0.0	2.694614593864426	0.0
23	4.2478357276967384E-4	0.0	0.0	2.8139787778127046	0.0
24	4.2478357276967384E-4	0.0	0.0	2.906793988462878	0.0
25	4.2478357276967384E-4	0.0	0.0	2.9753965354651806	0.0
26	4.2478357276967384E-4	0.0	0.0	3.0510080114181823	0.0
27	4.2478357276967384E-4	0.0	0.0	3.1264070955847996	0.0
28	4.2478357276967384E-4	0.0	0.0	3.20414248940165	0.0
29	4.2478357276967384E-4	0.0	0.0	3.289523987528354	0.0
30	4.2478357276967384E-4	0.0	0.0	3.418658193650335	0.0
31	4.2478357276967384E-4	0.0	0.0	3.5116857960868937	0.0
32	4.2478357276967384E-4	0.0	0.0	3.6059877492417614	0.0
33	4.2478357276967384E-4	0.0	0.0	3.6864842362816144	0.0
34	4.2478357276967384E-4	0.0	0.0	3.780998581222867	0.0
35	4.2478357276967384E-4	0.0	0.0	3.912681488781466	0.0
36	4.2478357276967384E-4	0.0	0.0	4.018452598401114	0.0
37	4.2478357276967384E-4	0.0	0.0	4.11487846941983	0.0
38	4.2478357276967384E-4	0.0	0.0	4.208755639001929	0.0
39	4.2478357276967384E-4	0.0	0.0	4.3047567264478745	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	30	2.1627602E-6	45.000004	6
TCTTGCG	30	2.1627602E-6	45.000004	1
AGTCCAT	30	2.1627602E-6	45.000004	38
CTAAGCG	30	2.1627602E-6	45.000004	1
GTAATCC	45	3.8380676E-10	45.000004	9
TCGCTAT	30	2.1627602E-6	45.000004	23
TCGAGAG	45	3.8380676E-10	45.000004	1
TAGTGCG	30	2.1627602E-6	45.000004	1
CAATACG	30	2.1627602E-6	45.000004	1
TGTCACG	20	7.02904E-4	45.0	1
GGTACGA	25	3.887285E-5	45.0	21
CACGGGT	25	3.887285E-5	45.0	4
CGACGGT	85	0.0	45.0	28
TCCGATC	20	7.02904E-4	45.0	14
GACGTCG	25	3.887285E-5	45.0	19
GACCGAA	20	7.02904E-4	45.0	9
CCTACTG	20	7.02904E-4	45.0	24
AAGCGAC	25	3.887285E-5	45.0	15
GGATACC	20	7.02904E-4	45.0	8
ACGGGTA	50	2.1827873E-11	45.0	5
>>END_MODULE