FastQCFastQC Report
Thu 26 May 2016
SRR1545514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545514_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4957030
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254250.5129079307569251No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC147430.29741599304422206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1350.045.0000041
CATACGT253.8926824E-545.00000444
GCGTAAT600.044.9999969
CGTTTTT170200.041.3381351
CGTAAGG6250.040.6799962
TACGTAA451.9304935E-840.014
TATAGCG2650.039.9056631
ACATACG14000.039.6964317
TAGGGCG19400.039.6649445
CGGTCTA7150.039.6503531
CGTCGAA403.4613186E-739.37527
ACGGGAT11950.039.163185
GGTACCT36950.039.1542638
TACGGGA11800.039.0889854
TAGCATA34900.039.0687730
GCTACGA5650.039.0265510
GACCGAT30900.038.810689
CAATTCG356.2524778E-638.571431
CGGATTA356.2524778E-638.5714331
ACTCGAT356.2524778E-638.571438
Produced by FastQC (version 0.11.5)