FastQCFastQC Report
Thu 26 May 2016
SRR1545513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545513_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2310158
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211200.9142231830030674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT162700.042.3724631
CGTAAGG3150.041.4285742
GCGTAAG1750.039.8571431
ATAACGC2250.039.011
CGGTCTA1800.038.7531
TAACGGG5250.038.571433
GTTGATC15300.037.6470616
GGGCGAT48150.037.5233657
TAGGGAC19250.037.2857175
ACGATAG1150.037.1739161
TATTACG552.752131E-936.818181
TTAACGG2150.036.6279032
ATAGGGC10400.036.5625044
GTTTTTT200950.036.4120452
GGTACCT16500.036.4090928
CATATGC15650.036.37380233
CGCATTA250.002107548536.016
ACGGGAT7500.036.05
ACGCTTA250.002107548536.023
AGGGCGA25100.035.9462176
Produced by FastQC (version 0.11.5)