FastQCFastQC Report
Thu 26 May 2016
SRR1545512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545512_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3217409
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139790.43448004279219704No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC112180.34866564990649307No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT37380.1161804420886496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGC207.035181E-445.00000416
GTTCGAC406.8212103E-945.0000044
TACGACA207.035181E-445.00000431
CGTTACA453.8562575E-1045.028
TCGACGA253.892377E-545.044
TAATACG1150.044.9999961
AGTCGAC302.1667256E-644.99999644
CGACGGT3350.042.98507328
CTCACGA3300.042.95454824
GCGTATG2200.042.9545441
TATCGTG1700.042.352941
CGCATCG2300.042.06521621
CCGTATG750.042.035
AATACGG2900.041.896552
AGATTCG603.6379788E-1241.2499961
CGTTTTT116550.041.2355231
ATGTACG2250.041.0000041
CACAACG556.184564E-1140.90909212
CAATCGT556.184564E-1140.90909233
CATACGA10100.040.76732618
Produced by FastQC (version 0.11.5)