FastQCFastQC Report
Thu 26 May 2016
SRR1545511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545511_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2181251
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142980.6554954014920795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23260.10663605426427313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107900.042.8104741
GCGTAAG1500.040.51
ACGGGAT13550.040.18455
GCGTTAG1600.039.3750041
CGTTAGG3050.039.098362
CGACGGT750.039.028
TACGGGA10550.038.8151664
AGGGCGA21200.038.419816
GGCACCG10200.038.1617668
TAGGGCG9350.037.7807465
GAACGGG6450.037.6744163
GGGCGAT42900.037.6048937
AAGGGAT43350.036.9550175
TAGGGAC23400.036.9230775
GTCGACG552.752131E-936.818181
GCCCTAC10950.036.78082326
TAGGGAT43800.036.729455
TGCGGGA17350.036.7002874
CTACGGG5100.036.617653
GGGACCG16300.036.5797547
Produced by FastQC (version 0.11.5)