##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545511_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2181251
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.063921116826997	31.0	31.0	33.0	30.0	34.0
2	31.360574275954487	31.0	31.0	34.0	30.0	34.0
3	31.446532746575244	31.0	31.0	34.0	30.0	34.0
4	35.224162647948354	37.0	35.0	37.0	33.0	37.0
5	35.09659227663391	37.0	35.0	37.0	32.0	37.0
6	35.13843523739359	37.0	35.0	37.0	32.0	37.0
7	35.777327322715266	37.0	35.0	37.0	35.0	37.0
8	35.78081477097317	37.0	35.0	37.0	35.0	37.0
9	37.42435648167038	39.0	37.0	39.0	35.0	39.0
10	36.611997885617015	39.0	35.0	39.0	32.0	39.0
11	36.28435241978113	38.0	35.0	39.0	31.0	39.0
12	35.96923371037996	37.0	35.0	39.0	31.0	39.0
13	35.84996132953062	38.0	35.0	39.0	30.0	39.0
14	36.880786301072185	39.0	35.0	41.0	31.0	41.0
15	37.044188862262985	39.0	35.0	41.0	31.0	41.0
16	37.07374025272653	39.0	35.0	41.0	32.0	41.0
17	36.98648023542454	38.0	35.0	41.0	31.0	41.0
18	36.84873313525129	38.0	35.0	40.0	31.0	41.0
19	36.80113613701495	38.0	35.0	40.0	31.0	41.0
20	36.68986283559297	38.0	35.0	40.0	31.0	41.0
21	36.5961964028899	38.0	35.0	40.0	31.0	41.0
22	36.495364357426084	38.0	35.0	40.0	30.0	41.0
23	36.41113792039522	38.0	35.0	40.0	30.0	41.0
24	36.31981601383793	38.0	35.0	40.0	30.0	41.0
25	36.22565307706449	38.0	35.0	40.0	30.0	41.0
26	36.086169817228736	38.0	34.0	40.0	30.0	41.0
27	35.9968288839753	38.0	34.0	40.0	30.0	41.0
28	35.96824299450178	38.0	34.0	40.0	30.0	41.0
29	35.94909389153289	38.0	34.0	40.0	30.0	41.0
30	35.852906428466966	38.0	34.0	40.0	29.0	41.0
31	35.72897112711925	38.0	34.0	40.0	29.0	41.0
32	35.5629856444765	38.0	34.0	40.0	28.0	41.0
33	35.35181324845238	38.0	34.0	40.0	27.0	41.0
34	35.16193230398519	38.0	34.0	40.0	26.0	41.0
35	35.00007518621195	38.0	34.0	40.0	25.0	41.0
36	34.873093926375276	38.0	34.0	40.0	24.0	41.0
37	34.839153770015464	38.0	33.0	40.0	24.0	41.0
38	34.761824751025905	38.0	33.0	40.0	24.0	41.0
39	34.71211657897234	38.0	33.0	40.0	24.0	41.0
40	34.61193599452791	37.0	33.0	40.0	24.0	41.0
41	34.551277225775486	37.0	33.0	40.0	23.0	41.0
42	34.4903539299237	37.0	33.0	40.0	23.0	41.0
43	34.44752117019087	37.0	33.0	40.0	23.0	41.0
44	34.336897037525716	37.0	33.0	40.0	23.0	41.0
45	34.24631278106004	37.0	33.0	40.0	23.0	41.0
46	34.18017733860065	37.0	33.0	40.0	23.0	41.0
47	34.11693014696612	37.0	33.0	40.0	23.0	41.0
48	34.02621110546196	37.0	33.0	40.0	23.0	41.0
49	33.981466140302054	36.0	33.0	40.0	23.0	41.0
50	33.95067646960391	36.0	33.0	40.0	23.0	41.0
51	33.80868684988568	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	10.0
14	13.0
15	62.0
16	209.0
17	637.0
18	1337.0
19	2571.0
20	4566.0
21	6984.0
22	10234.0
23	14986.0
24	20924.0
25	29426.0
26	38449.0
27	44689.0
28	48989.0
29	55746.0
30	64864.0
31	78138.0
32	95319.0
33	118170.0
34	168861.0
35	217424.0
36	180237.0
37	218262.0
38	303793.0
39	456152.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.611125908939414	24.044825652801993	28.084800878028247	16.259247560230346
2	32.10309129944238	25.654360731525166	28.788204567012233	13.454343402020216
3	27.674531725143048	25.71891084519847	32.983778574771996	13.622778854886485
4	24.857111813358483	28.74836504372949	31.881796271955864	14.512726870956163
5	22.746213067638706	32.393062513209166	30.47049605937143	14.390228359780693
6	20.487784303594587	41.11026195518077	27.922554534072418	10.479399207152227
7	87.34506024295231	3.4746115875706187	7.654506519423945	1.5258216500531119
8	88.04511722859955	2.8700044148976893	7.494231521269216	1.5906468352335426
9	82.1493720805171	5.842289585196752	9.239147626751805	2.7691907075343463
10	36.529450301684676	38.062744727681505	14.94550604217488	10.462298928458944
11	28.556961120017828	24.173788344395028	31.56275916893562	15.706491366651523
12	28.458439675213903	23.28063116074216	31.6408794769607	16.620049687083238
13	21.305709430047255	30.217934570574407	31.79643241424302	16.67992358513532
14	17.643040622101722	32.96103933018254	31.654449671312474	17.741470376403264
15	17.14112681209086	26.304583929130576	39.703454577212796	16.850834681565765
16	19.357882242804703	24.524091908725772	38.112349289467375	18.00567655900215
17	19.829721568035957	23.54534164110412	32.06416868118341	24.560768109676513
18	21.383279595058067	24.74281960214574	34.47374923839576	19.400151564400428
19	25.196962660418265	25.562807764901883	30.005441831316066	19.234787743363785
20	26.173512355982876	25.68063006045613	30.016903144113172	18.128954439447824
21	23.327347471703163	26.664148234201384	31.579813602377715	18.428690691717733
22	22.75194372403726	25.609799147370016	29.833797210866607	21.804459917726113
23	19.90316566043981	28.731906598552847	30.42396312941518	20.940964611592154
24	19.19985366195821	25.607644420564164	36.15241895591108	19.04008296156655
25	19.606776111506655	27.775987266023026	33.24573834006265	19.371498282407664
26	19.394466753253063	31.01183678540434	29.79689178365993	19.79680467768267
27	18.52973362533702	30.446198076241572	31.577842256576616	19.446226041844795
28	18.48815198250912	28.237052957225007	35.17380622404299	18.100988836222882
29	19.06419756369166	26.119414959580535	34.37646561537393	20.439921861353874
30	19.671876368194216	28.584835032740386	32.28257545784506	19.46071314122034
31	23.047691439453782	27.328766840679958	30.061373037765943	19.562168682100317
32	24.002785557462207	27.732067515384518	29.974037834251995	18.291109092901277
33	23.623737020636323	28.08865187912808	29.351092561103698	18.936518539131903
34	20.316231373647508	27.727162073507362	30.986117599487635	20.9704889533575
35	20.23123427794417	27.54309338998584	31.667790639408306	20.557881692661688
36	24.110200980996684	28.098050155621706	28.86151112366252	18.93023773971909
37	20.90763511397817	30.707516008015585	29.545568116644986	18.839280761361255
38	20.433457680936307	30.390266869791695	29.017889275466235	20.158386173805766
39	20.80824260940167	30.204410221473825	28.944857790323077	20.04248937880143
40	22.470591417493907	28.1313338079845	29.0334766608703	20.364598113651294
41	19.043475510154494	27.621947222029924	30.00154498496505	23.33303228285053
42	20.917125080974174	28.414061472063505	28.03221637491513	22.636597072047188
43	20.779910244167223	28.536789209494916	28.92294376025501	21.76035678608285
44	20.737732613073874	29.353980811928565	29.869052209030507	20.039234365967054
45	19.25248401032252	32.208351996171004	27.88308177279919	20.65608222070729
46	20.43831727756228	30.962553140376787	28.799940951316472	19.799188630744467
47	20.57846621044529	28.78242806536249	29.575550910922217	21.06355481327
48	21.28141144691739	27.018256954380764	30.706920019750132	20.99341157895171
49	20.546924677627654	27.810967192679797	31.064833895778154	20.57727423391439
50	19.446271887095985	30.679321178534703	29.58637039020269	20.288036544166626
51	19.578581282025773	30.82098300470693	28.764319191143063	20.836116522124232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1532.0
1	2124.0
2	2716.0
3	14020.5
4	25325.0
5	18790.0
6	12255.0
7	12334.0
8	12413.0
9	12886.0
10	13359.0
11	13170.5
12	12982.0
13	12868.0
14	12754.0
15	12645.0
16	12536.0
17	11965.0
18	11394.0
19	11579.5
20	11765.0
21	11747.0
22	11729.0
23	13953.0
24	16177.0
25	19237.5
26	26452.0
27	30606.0
28	36222.5
29	41839.0
30	49193.5
31	56548.0
32	64580.0
33	72612.0
34	81934.0
35	91256.0
36	99972.0
37	108688.0
38	115205.0
39	121722.0
40	135776.0
41	149830.0
42	162313.0
43	174796.0
44	172917.5
45	171039.0
46	168380.5
47	165722.0
48	158694.0
49	151666.0
50	145596.5
51	139527.0
52	130123.5
53	120720.0
54	108506.0
55	96292.0
56	87219.5
57	78147.0
58	70795.0
59	63443.0
60	59207.5
61	54972.0
62	46155.5
63	37339.0
64	31135.5
65	24932.0
66	21017.5
67	17103.0
68	14431.5
69	11760.0
70	9709.0
71	7658.0
72	6803.0
73	5948.0
74	4643.5
75	2768.5
76	2198.0
77	1645.5
78	1093.0
79	900.0
80	707.0
81	462.0
82	217.0
83	219.0
84	221.0
85	131.0
86	41.0
87	34.5
88	28.0
89	15.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2181251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.898776873450117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65976238373138	15.453342837190254
2	5.9721679187072025	2.3767767373026323
3	2.4377499717516486	1.4552472828343617
4	1.3817941394631186	1.0998405306487045
5	0.9858245937238143	0.9808351813434898
6	0.7318893613375622	0.8738221858384836
7	0.5802636844037105	0.808257630860086
8	0.46021776977654494	0.7326216571184243
9	0.39546886900226064	0.7082412106204494
>10	6.642357968610094	33.68718293320049
>50	2.465225021300068	33.877041029864834
>100	0.2863531066371071	7.0824141318553675
>500	0.0	0.0
>1k	6.939086665984045E-4	0.20629052988429242
>5k	0.0	0.0
>10k+	2.3130288886613485E-4	0.658086121438111
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14298	0.6554954014920795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2326	0.10663605426427313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017237814446847244	0.0
2	0.0	0.0	0.0	0.06899710303857741	0.0
3	0.0	0.0	0.0	0.10438963695604037	0.0
4	0.0	0.0	0.0	0.1397363256223149	0.0
5	0.0	0.0	0.0	0.2285844224254797	0.0
6	0.0	0.0	0.0	0.3921144334145864	0.0
7	0.0	0.0	0.0	0.48288803076766496	0.0
8	0.0	0.0	0.0	0.6921716024428184	0.0
9	0.0	0.0	0.0	0.8196672459978241	0.0
10	0.0	0.0	0.0	1.0171227428663643	0.0
11	0.0	0.0	0.0	1.1576384377588824	0.0
12	0.0	0.0	0.0	1.2872888081197442	0.0
13	0.0	0.0	0.0	1.3453747413754766	0.0
14	0.0	0.0	0.0	1.3756784524110246	0.0
15	0.0	0.0	0.0	1.4133174036367204	0.0
16	0.0	0.0	0.0	1.4845150787323422	0.0
17	0.0	0.0	0.0	1.5653861018287212	0.0
18	0.0	0.0	0.0	1.6931568168908575	0.0
19	0.0	0.0	0.0	1.7452370222409066	0.0
20	4.584525118842352E-5	0.0	0.0	1.811941862720063	0.0
21	4.584525118842352E-5	0.0	0.0	1.8800679059860603	0.0
22	4.584525118842352E-5	0.0	0.0	1.958188214011134	0.0
23	4.584525118842352E-5	0.0	0.0	2.0445148219989355	0.0
24	4.584525118842352E-5	0.0	0.0	2.115024818326731	0.0
25	4.584525118842352E-5	0.0	0.0	2.1754947046442616	0.0
26	4.584525118842352E-5	0.0	0.0	2.2322969708667184	0.0
27	4.584525118842352E-5	0.0	0.0	2.2970763107959606	0.0
28	4.584525118842352E-5	0.0	0.0	2.3630934725072907	0.0
29	4.584525118842352E-5	0.0	0.0	2.433603468835086	0.0
30	4.584525118842352E-5	0.0	0.0	2.5377180342839956	0.0
31	4.584525118842352E-5	0.0	0.0	2.6185432121291865	0.0
32	4.584525118842352E-5	0.0	0.0	2.698955782713681	0.0
33	4.584525118842352E-5	0.0	0.0	2.778451448274408	0.0
34	4.584525118842352E-5	0.0	0.0	2.859735078631483	0.0
35	4.584525118842352E-5	0.0	0.0	2.956468558639056	0.0
36	4.584525118842352E-5	0.0	0.0	3.043620381148249	0.0
37	4.584525118842352E-5	0.0	0.0	3.129442691372978	0.0
38	9.169050237684704E-5	0.0	0.0	3.2143480965739384	0.0
39	9.169050237684704E-5	0.0	0.0	3.3076431827423804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10790	0.0	42.810474	1
GCGTAAG	150	0.0	40.5	1
ACGGGAT	1355	0.0	40.1845	5
GCGTTAG	160	0.0	39.375004	1
CGTTAGG	305	0.0	39.09836	2
CGACGGT	75	0.0	39.0	28
TACGGGA	1055	0.0	38.815166	4
AGGGCGA	2120	0.0	38.41981	6
GGCACCG	1020	0.0	38.161766	8
TAGGGCG	935	0.0	37.780746	5
GAACGGG	645	0.0	37.674416	3
GGGCGAT	4290	0.0	37.604893	7
AAGGGAT	4335	0.0	36.955017	5
TAGGGAC	2340	0.0	36.923077	5
GTCGACG	55	2.752131E-9	36.81818	1
GCCCTAC	1095	0.0	36.780823	26
TAGGGAT	4380	0.0	36.72945	5
TGCGGGA	1735	0.0	36.700287	4
CTACGGG	510	0.0	36.61765	3
GGGACCG	1630	0.0	36.579754	7
>>END_MODULE