FastQCFastQC Report
Thu 26 May 2016
SRR1545510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545510_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1525818
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.3988024784083029No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39540.259139687695387No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24320.1593899141313053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT302.1659707E-645.00000419
TGCGCTA207.034013E-445.00000441
CGTAGAT302.1659707E-645.00000435
CTATTCG207.034013E-445.00000427
TTAGTCG207.034013E-445.0000041
TAGTCCG207.034013E-445.0000041
GTACGCC207.034013E-445.00000412
TACCGAA207.034013E-445.0000048
CAACCGG253.891409E-545.02
GTAGCGT351.2122837E-745.014
GTATGCG351.2122837E-745.01
GTCGTTA253.891409E-545.014
TTACGTA351.2122837E-745.027
TAAACGG2050.042.804882
TAGAACG950.042.631581
CGTTTTT48300.042.0652161
TAATGCG750.042.01
CGTAAGG2150.041.8604662
TGACCGG603.6379788E-1241.2500042
GCTACGA3050.040.57377210