##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545510_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1525818
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.079382993253454	31.0	31.0	33.0	30.0	34.0
2	31.36021203053051	31.0	31.0	34.0	30.0	34.0
3	31.47442552126138	31.0	31.0	34.0	30.0	34.0
4	35.24207277670076	37.0	35.0	37.0	33.0	37.0
5	35.098013000239874	37.0	35.0	37.0	32.0	37.0
6	35.143019678624846	37.0	35.0	37.0	32.0	37.0
7	35.75907742601018	37.0	35.0	37.0	35.0	37.0
8	35.76064969740821	37.0	35.0	37.0	35.0	37.0
9	37.419887561950375	39.0	37.0	39.0	35.0	39.0
10	36.61327825468044	39.0	35.0	39.0	32.0	39.0
11	36.280894575893065	38.0	35.0	39.0	32.0	39.0
12	35.37295208209629	37.0	35.0	39.0	30.0	39.0
13	34.94648575387104	37.0	34.0	39.0	28.0	39.0
14	35.85589762343871	38.0	34.0	40.0	29.0	41.0
15	36.222886346864435	38.0	35.0	40.0	30.0	41.0
16	36.401142206999786	38.0	35.0	40.0	31.0	41.0
17	36.35847132488934	38.0	35.0	40.0	31.0	41.0
18	36.299671389379334	37.0	35.0	40.0	31.0	41.0
19	36.2273606681793	37.0	35.0	40.0	31.0	41.0
20	36.10658348505523	37.0	34.0	40.0	30.0	41.0
21	35.94706052753343	37.0	34.0	40.0	30.0	41.0
22	35.825824574097304	37.0	34.0	40.0	30.0	41.0
23	35.77978435173789	37.0	34.0	40.0	30.0	41.0
24	35.70788193611558	36.0	34.0	40.0	30.0	41.0
25	35.612904029183035	36.0	34.0	40.0	30.0	41.0
26	35.44408835129747	36.0	34.0	40.0	29.0	41.0
27	35.35458554034623	36.0	34.0	40.0	29.0	41.0
28	35.37317491339072	36.0	34.0	40.0	29.0	41.0
29	35.414847642379364	36.0	34.0	40.0	29.0	41.0
30	35.36427345856452	36.0	34.0	40.0	29.0	41.0
31	35.20696111856067	36.0	34.0	40.0	29.0	41.0
32	35.039007273475605	36.0	34.0	40.0	28.0	41.0
33	34.85715727563838	36.0	33.0	40.0	27.0	41.0
34	34.755573731598396	36.0	34.0	40.0	27.0	41.0
35	34.6159384671042	36.0	33.0	40.0	26.0	41.0
36	34.407474548078476	36.0	33.0	40.0	24.0	41.0
37	34.352485683089334	35.0	33.0	40.0	24.0	41.0
38	34.34809852813376	35.0	33.0	40.0	25.0	41.0
39	34.30504490050583	35.0	33.0	40.0	24.0	41.0
40	34.150369179023976	35.0	33.0	40.0	24.0	41.0
41	34.14458867309207	35.0	33.0	40.0	24.0	41.0
42	34.0840480319409	35.0	33.0	40.0	24.0	41.0
43	34.021888586974335	35.0	33.0	40.0	24.0	41.0
44	33.931697620555006	35.0	33.0	40.0	23.0	41.0
45	33.861847218999905	35.0	33.0	39.0	23.0	41.0
46	33.80392156862745	35.0	33.0	39.0	23.0	41.0
47	33.713676205156844	35.0	33.0	39.0	23.0	41.0
48	33.67384773282266	35.0	33.0	39.0	23.0	41.0
49	33.66195312940338	35.0	32.0	39.0	24.0	41.0
50	33.608664336113485	35.0	32.0	39.0	24.0	41.0
51	33.42924451015783	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	14.0
15	56.0
16	156.0
17	431.0
18	998.0
19	1812.0
20	3164.0
21	4999.0
22	7548.0
23	10721.0
24	14982.0
25	20473.0
26	26500.0
27	31826.0
28	36127.0
29	41841.0
30	50079.0
31	61201.0
32	75390.0
33	96362.0
34	154078.0
35	217229.0
36	111484.0
37	132557.0
38	173400.0
39	252230.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82501582757577	23.064218668281537	29.777011412894595	14.333754091248105
2	30.00252979057791	26.635548931786097	30.31331390768755	13.048607369948447
3	28.516900442909964	26.57918572201927	30.857349959169444	14.046563875901318
4	25.788527858499506	27.868920146439486	31.067007991778834	15.275544003282175
5	22.562061792428718	33.38465006966755	30.175551736838862	13.877736401064872
6	20.847506059045052	41.59250972265369	27.247876221148264	10.312107997153003
7	87.89069207467732	4.33066066857253	6.045216401956196	1.733430854793953
8	89.11941004759414	3.4212468328463816	5.659914878445528	1.7994282411139466
9	84.04921163598804	5.280249675911544	7.702229230484894	2.9683094576155216
10	49.603491373151975	26.716095890859854	12.890266073673267	10.790146662314902
11	44.887267026604746	21.367817131532068	20.410691183352142	13.33422465851104
12	41.23938765960292	20.636602792731505	23.79995517158665	14.324054376078928
13	21.533302136952116	39.965710196104645	23.475276867883327	15.025710799059913
14	16.18095998343184	41.60299590121495	26.631157844513563	15.58488627083964
15	14.967774662508898	23.842030963063745	46.20347905189217	14.98671532253519
16	16.488663785589107	18.877349723230424	45.56939294201536	19.064593549165103
17	16.893626893902155	19.026646690496506	29.053792785246994	35.02593363035434
18	20.51725697298105	22.63762781668587	34.745035122144316	22.10008008818876
19	28.995987725927996	23.322768508432855	26.285834876767737	21.395408888871412
20	31.716954446729556	22.818842089947818	25.017203886702085	20.446999576620538
21	22.776962914318748	27.998031219975122	27.938128924943868	21.286876940762266
22	23.187103573296422	25.003637393188438	24.807611392708697	27.001647640806443
23	19.934618676670482	30.267109183401953	25.003637393188438	24.794634746739124
24	19.77195183173878	23.549335503972298	38.64504154492869	18.03367111936024
25	18.00974952451734	24.724377350378617	36.486658303939265	20.77921482116478
26	17.89971018824001	32.992532530092056	27.631408202026712	21.476349079641217
27	18.64291809377003	33.48466199769567	27.887336497537714	19.985083410996594
28	16.16149501447748	28.694116860595432	37.08083139666723	18.063556728259858
29	16.633700742814675	24.23572142942343	35.92984222233582	23.200735605426072
30	18.602415229077124	30.957296348581547	30.264422100145627	20.175866322195702
31	27.8872054203057	26.813420735631645	24.871446004700427	20.42792783936223
32	30.171357265414358	25.59223970355573	26.277183779454695	17.95921925157522
33	28.15250573790583	26.711114956043254	24.97506255660898	20.161316749441937
34	20.386179740965176	27.12341838934919	27.40392366586316	25.086478203822477
35	20.649055129773014	24.825437896262855	30.66106180422567	23.864445169738463
36	30.22038015018829	24.626003887750702	25.970004286225485	19.183611675835518
37	21.628333130163625	31.81958791939799	28.63598410819639	17.916094842241996
38	21.38760979356647	31.47891819338873	24.696195745495203	22.437276267549603
39	20.667143787791204	31.708958735576587	26.608022713062763	21.015874763569443
40	24.929709834331486	25.82654025578411	25.531223252052342	23.71252665783206
41	17.90272496457638	24.77864332443319	28.20788586843254	29.110745842557893
42	20.979042061372983	25.981408005410866	25.77961460672243	27.25993532649372
43	21.954125590339082	26.44502817505102	26.51430249217141	25.086543742438483
44	20.433302005874882	28.83725319795677	28.43425624812396	22.295188548044393
45	18.434046524552734	35.87963964247374	23.550253044596406	22.136060788377122
46	21.5464098601537	32.460424506723605	25.963384886008683	20.029780747114007
47	21.896517146868106	27.424240636825626	26.760137840817187	23.91910437548908
48	22.974823996046712	23.803101025155033	30.591918564337295	22.630156414460963
49	21.274490142336766	24.148161838436824	31.99457602413918	22.582771995087224
50	20.578470040332462	32.05736201827479	26.350718106615602	21.01344983477715
51	19.407294972270613	32.82167335815936	24.132235954746896	23.63879571482313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1221.0
1	1694.0
2	2167.0
3	6333.0
4	10499.0
5	7821.5
6	5144.0
7	5428.0
8	5712.0
9	6002.5
10	6293.0
11	6392.0
12	6491.0
13	6442.0
14	6393.0
15	6077.5
16	5762.0
17	5685.0
18	5608.0
19	5176.0
20	4744.0
21	5290.5
22	5837.0
23	5881.0
24	5925.0
25	6787.0
26	9558.5
27	11468.0
28	15384.5
29	19301.0
30	22166.5
31	25032.0
32	29041.5
33	33051.0
34	38788.5
35	44526.0
36	42869.0
37	41212.0
38	51513.5
39	61815.0
40	84155.0
41	106495.0
42	126720.0
43	146945.0
44	150411.0
45	153877.0
46	145389.5
47	136902.0
48	128769.5
49	120637.0
50	111705.0
51	102773.0
52	96367.0
53	89961.0
54	80205.0
55	70449.0
56	65552.0
57	60655.0
58	57690.0
59	54725.0
60	51926.0
61	49127.0
62	42369.0
63	35611.0
64	30210.0
65	24809.0
66	21396.0
67	17983.0
68	15448.5
69	12914.0
70	11323.5
71	9733.0
72	8301.0
73	6869.0
74	5751.0
75	3499.0
76	2365.0
77	1768.0
78	1171.0
79	1063.0
80	955.0
81	600.5
82	246.0
83	173.0
84	100.0
85	60.0
86	20.0
87	14.5
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1525818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.136602031500065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82898843351587	13.679976053623832
2	6.804034186337951	2.331960521199896
3	2.309100678563253	1.1871041813761565
4	1.2006112098749668	0.8229758599274042
5	0.7292659309641722	0.6248570017032208
6	0.5099151782195169	0.5242928087381575
7	0.39895750611483116	0.47857432068387296
8	0.3297516773601716	0.4520658611312697
9	0.2793779074024139	0.4308829213993599
>10	4.12609084462527	17.85099840830347
>50	2.0565470657596485	25.678629009524833
>100	1.423144360270306	34.75959320321705
>500	0.003065469812106206	0.3591857463334226
>1k	7.663674530265515E-4	0.41933458429346193
>5k	3.8318372651327575E-4	0.39956951854458433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6085	0.3988024784083029	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3954	0.259139687695387	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2432	0.1593899141313053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03145853568381026	0.0
2	0.0	0.0	0.0	0.11344734430974074	0.0
3	0.0	0.0	0.0	0.1589966824352577	0.0
4	0.0	0.0	0.0	0.2546175231908393	0.0
5	0.0	0.0	0.0	0.428360394227883	0.0
6	0.0	0.0	0.0	0.6364455000530863	0.0
7	0.0	0.0	0.0	0.7573642465877319	0.0
8	0.0	0.0	0.0	1.08099393243493	0.0
9	0.0	0.0	0.0	1.2062382276260997	0.0
10	0.0	0.0	0.0	1.4261858229487396	0.0
11	0.0	0.0	0.0	1.5965206859533705	0.0
12	0.0	0.0	0.0	1.7576801427168902	0.0
13	0.0	0.0	0.0	1.8240052221169236	0.0
14	0.0	0.0	0.0	1.849892975440059	0.0
15	0.0	0.0	0.0	1.8905269173649806	0.0
16	0.0	0.0	0.0	1.982149902544078	0.0
17	0.0	0.0	0.0	2.07934367008385	0.0
18	0.0	0.0	0.0	2.2376194277430206	0.0
19	0.0	0.0	0.0	2.2924097107256567	0.0
20	0.0	0.0	0.0	2.35460585731719	0.0
21	0.0	0.0	0.0	2.4365291273271126	0.0
22	0.0	0.0	0.0	2.527955496658186	0.0
23	0.0	0.0	0.0	2.638977912175633	0.0
24	0.0	0.0	0.0	2.7188039464733014	0.0
25	0.0	0.0	0.0	2.7872262615855887	0.0
26	0.0	0.0	0.0	2.858925507498273	0.0
27	0.0	0.0	0.0	2.9231533511860524	0.0
28	0.0	0.0	0.0	2.9906581256742286	0.0
29	0.0	0.0	0.0	3.0699598510438335	0.0
30	0.0	0.0	0.0	3.1786228763849946	0.0
31	0.0	0.0	0.0	3.2712289408042112	0.0
32	0.0	0.0	0.0	3.353152210814134	0.0
33	0.0	0.0	0.0	3.4390733364005404	0.0
34	0.0	0.0	0.0	3.5278126224752886	0.0
35	0.0	0.0	0.0	3.6516150681142836	0.0
36	0.0	0.0	0.0	3.7497263762781667	0.0
37	0.0	0.0	0.0	3.848886302298177	0.0
38	0.0	0.0	0.0	3.939919439933203	0.0
39	0.0	0.0	0.0	4.031739040960324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	30	2.1659707E-6	45.000004	19
TGCGCTA	20	7.034013E-4	45.000004	41
CGTAGAT	30	2.1659707E-6	45.000004	35
CTATTCG	20	7.034013E-4	45.000004	27
TTAGTCG	20	7.034013E-4	45.000004	1
TAGTCCG	20	7.034013E-4	45.000004	1
GTACGCC	20	7.034013E-4	45.000004	12
TACCGAA	20	7.034013E-4	45.000004	8
CAACCGG	25	3.891409E-5	45.0	2
GTAGCGT	35	1.2122837E-7	45.0	14
GTATGCG	35	1.2122837E-7	45.0	1
GTCGTTA	25	3.891409E-5	45.0	14
TTACGTA	35	1.2122837E-7	45.0	27
TAAACGG	205	0.0	42.80488	2
TAGAACG	95	0.0	42.63158	1
CGTTTTT	4830	0.0	42.065216	1
TAATGCG	75	0.0	42.0	1
CGTAAGG	215	0.0	41.860466	2
TGACCGG	60	3.6379788E-12	41.250004	2
GCTACGA	305	0.0	40.573772	10
>>END_MODULE