FastQCFastQC Report
Thu 26 May 2016
SRR1545509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545509_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences160628
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.5297955524566078No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3660.22785566650895236No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2800.1743158104440073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAA650.045.0000045
TCACGAC207.0151547E-445.025
CTTAAGG253.8757826E-545.02
CTCACGA207.0151547E-445.024
ACGCCGG207.0151547E-445.027
CGACGGT207.0151547E-445.028
CATGAGG406.7520887E-945.02
CACGCCG207.0151547E-445.026
TGACGTG207.0151547E-445.013
CGCGACC207.0151547E-445.010
GTGGCGA207.0151547E-445.028
CGCTGAA207.0151547E-445.037
CGTTAGG207.0151547E-445.02
AGACGTG207.0151547E-445.024
AACGGGA453.8016879E-1045.04
ACCTATG406.7520887E-945.034
TAGGGTG351.2032251E-745.05
GACGGTC207.0151547E-445.029
CGGGACC207.0151547E-445.06
GCGATCA207.0151547E-445.09