##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545509_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	160628
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070436038548696	31.0	31.0	33.0	30.0	34.0
2	31.35827502054436	31.0	31.0	34.0	30.0	34.0
3	31.470478372388374	31.0	31.0	34.0	30.0	34.0
4	35.23362676494758	37.0	35.0	37.0	33.0	37.0
5	35.08796722862763	37.0	35.0	37.0	32.0	37.0
6	35.12678984983938	37.0	35.0	37.0	32.0	37.0
7	35.73395671987449	37.0	35.0	37.0	35.0	37.0
8	35.74945837587469	37.0	35.0	37.0	35.0	37.0
9	37.42081081754115	39.0	37.0	39.0	35.0	39.0
10	36.610952013347614	39.0	35.0	39.0	32.0	39.0
11	36.26637323505242	38.0	35.0	39.0	32.0	39.0
12	35.25514854197276	37.0	35.0	39.0	30.0	39.0
13	34.763042557960006	37.0	33.0	39.0	27.0	39.0
14	35.6393032348034	37.0	34.0	40.0	27.0	41.0
15	36.047295614712255	37.0	34.0	40.0	30.0	41.0
16	36.25362950419603	37.0	34.0	40.0	31.0	41.0
17	36.2052444156685	37.0	34.0	40.0	31.0	41.0
18	36.13208157979929	37.0	35.0	40.0	30.0	41.0
19	36.06762208332296	37.0	35.0	40.0	30.0	41.0
20	35.91480314764549	36.0	34.0	40.0	30.0	41.0
21	35.746495007097145	36.0	34.0	40.0	30.0	41.0
22	35.63818263316483	36.0	34.0	40.0	30.0	41.0
23	35.64128296436487	36.0	34.0	40.0	30.0	41.0
24	35.51080135468287	36.0	34.0	40.0	29.0	41.0
25	35.41296660607117	35.0	34.0	40.0	29.0	41.0
26	35.273140423836445	35.0	34.0	40.0	29.0	41.0
27	35.184351420673856	35.0	34.0	40.0	29.0	41.0
28	35.19460492566676	36.0	34.0	40.0	29.0	41.0
29	35.22436312473541	36.0	34.0	40.0	29.0	41.0
30	35.16316582414025	36.0	34.0	40.0	29.0	41.0
31	34.97380282391613	36.0	34.0	40.0	28.0	41.0
32	34.801952337076976	35.0	34.0	40.0	27.0	41.0
33	34.60760265956122	35.0	33.0	40.0	26.0	41.0
34	34.46556640187265	35.0	33.0	40.0	25.0	41.0
35	34.297768757626315	35.0	33.0	40.0	24.0	41.0
36	34.10965087033394	35.0	33.0	40.0	23.0	41.0
37	34.009462858281246	35.0	33.0	40.0	23.0	41.0
38	33.99876111263291	35.0	33.0	40.0	24.0	41.0
39	33.98271160694275	35.0	33.0	40.0	23.0	41.0
40	33.82993002465324	35.0	33.0	40.0	23.0	41.0
41	33.81519411310606	35.0	33.0	40.0	23.0	41.0
42	33.763926588141544	35.0	33.0	39.0	23.0	41.0
43	33.70538137809099	35.0	33.0	39.0	23.0	41.0
44	33.59536319944219	35.0	33.0	39.0	23.0	41.0
45	33.511884602933485	35.0	32.0	39.0	23.0	41.0
46	33.475259606046265	35.0	32.0	39.0	23.0	41.0
47	33.397577010234826	35.0	32.0	39.0	23.0	41.0
48	33.36648031476455	35.0	32.0	39.0	23.0	41.0
49	33.36739547276938	35.0	32.0	39.0	23.0	41.0
50	33.27741116119232	35.0	32.0	39.0	23.0	41.0
51	33.09751724481411	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	20.0
17	59.0
18	110.0
19	244.0
20	394.0
21	623.0
22	956.0
23	1320.0
24	1832.0
25	2369.0
26	3179.0
27	3505.0
28	4009.0
29	4552.0
30	5298.0
31	6557.0
32	8058.0
33	10130.0
34	16534.0
35	23934.0
36	11236.0
37	13046.0
38	17285.0
39	25352.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.225390342904106	24.054959284807133	28.7882560948278	14.931394277460965
2	31.20937819060189	26.270015190377766	28.97564559105511	13.544961027965236
3	27.764773264935126	25.272679732051696	32.72779341086237	14.23475359215081
4	24.755957865378388	28.879149338845032	31.150857882809973	15.214034912966607
5	23.050153148890605	32.016211370371295	30.644719476056476	14.288916004681623
6	20.66949722339816	41.34771023731852	27.352018328062357	10.630774211220958
7	87.10436536593869	4.395248649052469	6.755982767637024	1.7444032173718156
8	88.27228129591353	3.3698981497621836	6.445327091167169	1.9124934631571082
9	83.26381452797769	5.565032248424933	8.210274671912742	2.9608785516846376
10	51.17663172049704	24.301491645292227	13.733595637124289	10.788280997086435
11	47.32798764847972	20.177055058893842	19.613641457280178	12.881315835346266
12	43.48121124586	20.156510695520083	23.34835769604303	13.013920362576886
13	22.083945513858108	41.48280499041263	21.945115421968772	14.48813407376049
14	15.364693577707497	43.47374056826954	25.776327912941703	15.385237941081256
15	14.531713026371493	23.320342655078814	47.28440869586871	14.863535622680976
16	16.10678088502627	17.674378066090593	47.337948551933664	18.88089249694947
17	16.094952312174716	18.69537067011978	29.157432079089574	36.052244938615935
18	20.38249869263142	22.980426824713003	34.690713947755064	21.946360534900517
19	28.550439524864906	24.989416540080185	25.082800009960906	21.377343925094006
20	32.11706551784247	22.31180118036706	24.838757875339294	20.73237542645118
21	21.556640187264986	30.013447219662826	27.13225589560973	21.29765669746246
22	22.424483900689793	25.899594093184252	24.595338297183556	27.080583708942402
23	20.047563313992576	31.368752645864976	24.516273626017878	24.06741041412456
24	19.58936175511119	23.667729163035087	39.692332594566324	17.050576487287397
25	17.76278108424434	24.762805986502975	36.55713823243768	20.917274696815
26	16.744901262544513	34.891799686231536	26.613043803072937	21.750255248151007
27	17.73352093034838	34.54565829120701	27.497073984610402	20.223746793834202
28	15.906940259481535	28.90778693627512	37.72380904948079	17.46146375476256
29	16.531986951216478	24.228029982319395	36.997285653808795	22.24269741265533
30	18.06098563139677	31.873023383220854	30.34651492890405	19.71947605647832
31	29.268869686480564	27.201359663321462	24.537440545857507	18.992330104340464
32	30.611101426899417	26.114376073909906	26.1013023881266	17.173220111064076
33	28.230445501406976	27.32338073063227	24.831909754214706	19.614264013746048
34	19.508429414547898	26.957317528699853	28.57036133177279	24.963891724979455
35	19.898772318649304	25.928854247080206	31.96453918370396	22.207834250566528
36	30.39009388151505	26.05398809672037	24.098538237418136	19.45737978434644
37	20.15028513086137	33.59625968075304	27.82080334686356	18.432651841522027
38	21.277112334088702	33.283736334885575	24.84622855292975	20.592922778095975
39	20.351993425803723	33.17914684861917	25.74084219438703	20.72801753119008
40	25.492442164504318	25.31688124112857	24.82132629429489	24.369350300072217
41	17.17882311925692	24.362502178947633	27.488358194088203	30.970316507707253
42	21.339990537141716	26.82782578379859	23.83208407002515	28.00009960903454
43	21.386059715616206	27.14906492018826	25.663022636153098	25.801852728042434
44	20.072465572627436	29.334860671862938	28.679308713300294	21.91336504220933
45	16.66645914784471	36.933784894289914	23.42555469781109	22.974201260054286
46	20.691286699703664	33.81788978260328	25.416490275668004	20.07433324202505
47	21.770177055058895	27.37816569962896	26.713897950544112	24.137759294768035
48	22.13686281345718	23.39131409218816	31.259805264337476	23.21201783001718
49	20.83945513858107	24.206240506013895	32.63627761037926	22.318026745025772
50	19.72321139527355	33.68777548123615	26.016634708768084	20.572378414722216
51	18.31000821774535	34.327140971686134	24.28406006424783	23.078790746320692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	188.5
2	219.0
3	829.0
4	1439.0
5	1044.0
6	649.0
7	685.0
8	721.0
9	762.0
10	803.0
11	834.5
12	866.0
13	819.0
14	772.0
15	733.5
16	695.0
17	667.5
18	640.0
19	627.0
20	614.0
21	589.5
22	565.0
23	628.5
24	692.0
25	671.0
26	721.5
27	793.0
28	1326.0
29	1859.0
30	2053.5
31	2248.0
32	2778.5
33	3309.0
34	3780.5
35	4252.0
36	4543.0
37	4834.0
38	5667.5
39	6501.0
40	9059.5
41	11618.0
42	13804.0
43	15990.0
44	16300.0
45	16610.0
46	15430.0
47	14250.0
48	13468.0
49	12686.0
50	11680.5
51	10675.0
52	10230.5
53	9786.0
54	8776.5
55	7767.0
56	7051.5
57	6336.0
58	5865.5
59	5395.0
60	5075.0
61	4755.0
62	4050.0
63	3345.0
64	3027.0
65	2709.0
66	2193.0
67	1677.0
68	1511.5
69	1346.0
70	1145.0
71	944.0
72	758.0
73	572.0
74	462.5
75	303.0
76	253.0
77	201.0
78	149.0
79	110.5
80	72.0
81	49.0
82	26.0
83	28.0
84	30.0
85	17.0
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	160628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.53044301118111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1529098807724	22.82105236944991
2	6.202514688151877	4.035411011778768
3	3.423726867356898	3.3412605523320966
4	2.683099534954931	3.4912966606071167
5	2.2429334200907127	3.6481808900067234
6	1.7970260080760914	3.5074831287197745
7	1.649666047882418	3.7565057150683567
8	1.435324287600712	3.7353387952287274
9	1.2669129045222283	3.709191423662126
>10	9.119093640556523	46.57780710710461
>50	0.01913765716800949	0.36855342779590106
>100	0.0057412971504028475	0.47812336578927705
>500	0.0019137657168009493	0.5297955524566078
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.5297955524566078	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	366	0.22785566650895236	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	280	0.1743158104440073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02988271036182982	0.0
2	0.0	0.0	0.0	0.10583459919814728	0.0
3	0.0	0.0	0.0	0.15377144707024928	0.0
4	0.0	0.0	0.0	0.24840002988271037	0.0
5	0.0	0.0	0.0	0.4071519286799313	0.0
6	0.0	0.0	0.0	0.6580421844261275	0.0
7	0.0	0.0	0.0	0.7856662599297757	0.0
8	0.0	0.0	0.0	1.1181114127051324	0.0
9	0.0	0.0	0.0	1.2830888761610677	0.0
10	0.0	0.0	0.0	1.4984934133525911	0.0
11	0.0	0.0	0.0	1.670941554398984	0.0
12	0.0	0.0	0.0	1.8452573648429913	0.0
13	0.0	0.0	0.0	1.9218318101451801	0.0
14	0.0	0.0	0.0	1.9479791817117813	0.0
15	0.0	0.0	0.0	1.9797295614712254	0.0
16	0.0	0.0	0.0	2.0569265632392857	0.0
17	0.0	0.0	0.0	2.137858903802575	0.0
18	0.0	0.0	0.0	2.265482979306223	0.0
19	0.0	0.0	0.0	2.312174714246582	0.0
20	0.0	0.0	0.0	2.3769205866972136	0.0
21	0.0	0.0	0.0	2.447269467340688	0.0
22	0.0	0.0	0.0	2.53380481609682	0.0
23	0.0	0.0	0.0	2.6303010683068955	0.0
24	0.0	0.0	0.0	2.7056304006773413	0.0
25	0.0	0.0	0.0	2.7554349179470576	0.0
26	0.0	0.0	0.0	2.8158228951365887	0.0
27	0.0	0.0	0.0	2.8824364369848343	0.0
28	0.0	0.0	0.0	2.935353736583908	0.0
29	0.0	0.0	0.0	3.0025898348980253	0.0
30	0.0	0.0	0.0	3.095350748312872	0.0
31	0.0	0.0	0.0	3.1675672983539607	0.0
32	0.0	0.0	0.0	3.237916178997435	0.0
33	0.0	0.0	0.0	3.2927011479941233	0.0
34	0.0	0.0	0.0	3.3717658191597977	0.0
35	0.0	0.0	0.0	3.474487636028588	0.0
36	0.0	0.0	0.0	3.56102298478472	0.0
37	0.0	0.0	0.0	3.648803446472595	0.0
38	0.0	0.0	0.0	3.734716238762856	0.0
39	0.0	0.0	0.0	3.811913240530916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAA	65	0.0	45.000004	5
TCACGAC	20	7.0151547E-4	45.0	25
CTTAAGG	25	3.8757826E-5	45.0	2
CTCACGA	20	7.0151547E-4	45.0	24
ACGCCGG	20	7.0151547E-4	45.0	27
CGACGGT	20	7.0151547E-4	45.0	28
CATGAGG	40	6.7520887E-9	45.0	2
CACGCCG	20	7.0151547E-4	45.0	26
TGACGTG	20	7.0151547E-4	45.0	13
CGCGACC	20	7.0151547E-4	45.0	10
GTGGCGA	20	7.0151547E-4	45.0	28
CGCTGAA	20	7.0151547E-4	45.0	37
CGTTAGG	20	7.0151547E-4	45.0	2
AGACGTG	20	7.0151547E-4	45.0	24
AACGGGA	45	3.8016879E-10	45.0	4
ACCTATG	40	6.7520887E-9	45.0	34
TAGGGTG	35	1.2032251E-7	45.0	5
GACGGTC	20	7.0151547E-4	45.0	29
CGGGACC	20	7.0151547E-4	45.0	6
GCGATCA	20	7.0151547E-4	45.0	9
>>END_MODULE