FastQCFastQC Report
Thu 26 May 2016
SRR1545508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545508_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1901208
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80890.4254663350880072No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47470.24968335921161702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.034454E-445.01
GTATTCG253.8917715E-545.034
TCGATAG950.045.01
ACGGCTA207.034454E-445.036
ACGTATG502.1827873E-1145.01
TAGTACT207.034454E-445.043
TCAATCG302.1662545E-644.99999637
TACGTAA302.1662545E-644.99999619
AACGGGA3450.042.3913044
CGGTCTA1400.041.78571331
GTATACT650.041.5384637
ATTACGG1150.041.0869562
GCGATAT2500.040.5000049
GGTACCT13400.040.466428
CATATGC13250.040.07547433
TAACGGG5400.040.0000043
AGGGTAC7650.040.06
TAGCATA13300.039.75564230
GTTGATC13550.039.68634416
CGTTTTT60500.039.6818161
Produced by FastQC (version 0.11.5)