##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545508_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1901208
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07717040955014	31.0	31.0	33.0	30.0	34.0
2	31.351230901616237	31.0	31.0	34.0	30.0	34.0
3	31.473925525245	31.0	31.0	34.0	30.0	34.0
4	35.25335681314196	37.0	35.0	37.0	33.0	37.0
5	35.125507046046515	37.0	35.0	37.0	32.0	37.0
6	35.169971933633775	37.0	35.0	37.0	32.0	37.0
7	35.7376084047616	37.0	35.0	37.0	35.0	37.0
8	35.72244909552243	37.0	35.0	37.0	35.0	37.0
9	37.38100775927726	39.0	37.0	39.0	35.0	39.0
10	36.61043767962264	39.0	35.0	39.0	32.0	39.0
11	36.2750861557494	38.0	35.0	39.0	32.0	39.0
12	35.44647929106126	37.0	35.0	39.0	30.0	39.0
13	35.0779378163778	37.0	34.0	39.0	30.0	39.0
14	35.9925568375475	38.0	34.0	40.0	30.0	41.0
15	36.28645103534174	38.0	35.0	40.0	30.0	41.0
16	36.44296520948786	38.0	35.0	40.0	31.0	41.0
17	36.38580207951997	38.0	35.0	40.0	31.0	41.0
18	36.32852165570522	38.0	35.0	40.0	31.0	41.0
19	36.244746498016	37.0	35.0	40.0	30.0	41.0
20	36.136312281454735	37.0	34.0	40.0	30.0	41.0
21	35.99452874172631	37.0	34.0	40.0	30.0	41.0
22	35.86014628594031	37.0	34.0	40.0	30.0	41.0
23	35.80413505518597	37.0	34.0	40.0	30.0	41.0
24	35.722424374397754	37.0	34.0	40.0	30.0	41.0
25	35.59204042903249	36.0	34.0	40.0	29.0	41.0
26	35.41258452520713	36.0	34.0	40.0	29.0	41.0
27	35.32449211238328	36.0	34.0	40.0	29.0	41.0
28	35.35617617851387	36.0	34.0	40.0	29.0	41.0
29	35.352544803093615	36.0	34.0	40.0	29.0	41.0
30	35.28635320280579	36.0	34.0	40.0	29.0	41.0
31	35.14766664141956	36.0	34.0	40.0	28.0	41.0
32	34.992132896558395	36.0	34.0	40.0	27.0	41.0
33	34.811926417309415	36.0	33.0	40.0	27.0	41.0
34	34.66443124581845	36.0	33.0	40.0	26.0	41.0
35	34.526124968967096	36.0	33.0	40.0	25.0	41.0
36	34.34186264732738	36.0	33.0	40.0	24.0	41.0
37	34.28572360309866	35.0	33.0	40.0	24.0	41.0
38	34.244904292428814	35.0	33.0	40.0	24.0	41.0
39	34.19768904822618	35.0	33.0	40.0	24.0	41.0
40	34.010809443259234	35.0	33.0	40.0	23.0	41.0
41	33.98044453841978	35.0	33.0	40.0	23.0	41.0
42	33.90463431670811	35.0	33.0	40.0	23.0	41.0
43	33.849782874887964	35.0	33.0	40.0	23.0	41.0
44	33.73804181341547	35.0	33.0	39.0	23.0	41.0
45	33.66314837724226	35.0	33.0	39.0	23.0	41.0
46	33.592965104291586	35.0	32.0	39.0	23.0	41.0
47	33.51536707188272	35.0	32.0	39.0	23.0	41.0
48	33.459064973427424	35.0	32.0	39.0	23.0	41.0
49	33.42494982137673	35.0	32.0	39.0	23.0	41.0
50	33.37075427833251	35.0	32.0	39.0	23.0	41.0
51	33.20112265464905	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	3.0
14	18.0
15	71.0
16	182.0
17	508.0
18	1196.0
19	2469.0
20	4108.0
21	6582.0
22	9760.0
23	13738.0
24	19148.0
25	26208.0
26	33848.0
27	40742.0
28	46075.0
29	53766.0
30	64011.0
31	76939.0
32	94647.0
33	119844.0
34	192102.0
35	259062.0
36	144021.0
37	169748.0
38	220885.0
39	301340.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3984498276885	24.13723274886283	27.80011445354743	14.664202969901242
2	30.023069543153618	26.543071562922098	29.908405603174405	13.525453290749882
3	29.162195824970226	25.553753192706953	31.28610862146593	13.997942360856888
4	26.01346091537591	28.105341446070074	30.138417258921695	15.742780379632318
5	22.538512356354488	33.78930658823233	29.150256047733862	14.521925007679329
6	21.583119784894656	41.70343276485266	26.502308006278113	10.211139443974568
7	87.42441647626141	4.745666965424088	6.0150704183866255	1.8148461399278775
8	88.06942743771329	3.736098312230961	6.152509351948867	2.0419648981068876
9	83.22924161901275	5.750712178783174	7.991445438899899	3.028600763304173
10	48.714974900168734	25.60677211541294	13.664470168440277	12.013782815978052
11	44.69142776592566	21.69972985596526	19.306093809830383	14.302748568278695
12	40.40173405540057	20.25838309117151	24.08337225595516	15.256510597472763
13	23.289035181842284	35.43873158539203	24.117824036086528	17.15440919667916
14	17.424868820244814	38.32531737716231	26.865813735267263	17.384000067325616
15	16.906303781595703	22.64817947326121	44.15419038842673	16.29132635671636
16	18.753024392912295	17.994296257958098	43.002764558112524	20.24991479101708
17	18.42575878073309	18.350911630920972	29.11559387505207	34.10773571329386
18	22.178846291410515	21.844479930654614	33.687897378929605	22.288776399005265
19	29.493038110506582	23.35283672275732	25.234745488131754	21.91937967860434
20	31.840492991824142	22.624299918788477	24.353884477658415	21.181322611728966
21	24.726016301214806	26.949286979646626	26.397374721755853	21.927321997382716
22	24.95018956368793	24.11188044653715	24.118192223049768	26.819737766725154
23	21.620569658869517	28.82598852939815	24.504946328860388	25.048495482871942
24	21.500698503267397	23.08921485708034	36.50137175942874	18.90871488022352
25	20.116105128949595	24.164373387867084	33.76726796857577	21.952253514607555
26	19.11537296287413	30.995451313059906	26.930193855695954	22.958981868370003
27	19.98340002777182	31.189433244547676	27.81321138981111	21.013955337869398
28	18.109117992350125	27.89153001670517	34.661699298551234	19.337652692393466
29	18.693956684381718	24.283928954643574	33.7144068402826	23.30770752069211
30	20.203891420612578	29.385264526553645	29.614119023273624	20.796725029560154
31	28.785645757855004	25.81201004834821	24.611667950061225	20.79067624373556
32	30.1640325519354	25.46549351780552	25.84819756702055	18.52227636323853
33	28.263609242123955	26.420570500439723	24.362668366638474	20.953151890797848
34	21.378407833335437	26.530132421071233	27.487681516172874	24.603778229420453
35	22.51379123168007	24.5296148553972	29.50844936482489	23.44814454809784
36	29.441597131928752	25.977957172492438	24.38218227568998	20.19826341988883
37	21.92784797875877	31.46525787814905	26.83162494582392	19.775269197268265
38	22.938047809603155	30.635206668602276	23.72943938801015	22.697306133784416
39	22.154545951836937	29.47715347294983	25.742738301122237	22.625562274091
40	24.995949943404405	25.61571379880581	25.494106904662722	23.894229353127063
41	19.58591590188975	24.188252942339815	26.553433396030314	29.67239775974012
42	22.057975771193895	26.100353038699602	24.487431149037874	27.35424004106863
43	22.31223516837716	25.375550702500725	26.30869426175358	26.003519867368535
44	20.775107195004438	27.86407378887528	27.67335294191903	23.687466074201243
45	18.879680708265482	33.92032854900673	23.64007515221901	23.55991559050877
46	22.292879053738464	30.571089538861607	25.824475806960628	21.3115556004393
47	22.828065103870802	27.338355403511873	25.793811092736828	24.039768399880497
48	23.486804179237623	23.8506254970524	29.627110763262092	23.035459560447883
49	22.065234314183403	24.59220663914732	30.57661234331015	22.765946703359127
50	21.812289870440267	30.292109017003927	25.819636778300953	22.075964334254852
51	20.318870949417423	31.62699715128487	24.117981830499346	23.936150068798366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1280.0
1	2198.5
2	3117.0
3	8752.5
4	14388.0
5	10622.5
6	6857.0
7	7084.5
8	7312.0
9	7635.0
10	7958.0
11	8248.5
12	8539.0
13	8251.5
14	7964.0
15	7763.0
16	7562.0
17	7283.5
18	7005.0
19	6723.5
20	6442.0
21	6655.5
22	6869.0
23	7018.0
24	7167.0
25	8054.0
26	11876.5
27	14812.0
28	16744.5
29	18677.0
30	22365.0
31	26053.0
32	30458.5
33	34864.0
34	37964.5
35	41065.0
36	40861.5
37	40658.0
38	52281.0
39	63904.0
40	88829.0
41	113754.0
42	134876.5
43	155999.0
44	158243.0
45	160487.0
46	161124.0
47	161761.0
48	152287.5
49	142814.0
50	141722.0
51	140630.0
52	131515.0
53	122400.0
54	110466.5
55	98533.0
56	96714.0
57	94895.0
58	91085.5
59	87276.0
60	82547.5
61	77819.0
62	68419.0
63	59019.0
64	52131.0
65	45243.0
66	36743.5
67	28244.0
68	25216.5
69	22189.0
70	19134.0
71	16079.0
72	14614.0
73	13149.0
74	10936.0
75	7007.0
76	5291.0
77	3860.5
78	2430.0
79	1891.0
80	1352.0
81	1149.0
82	946.0
83	713.0
84	480.0
85	314.5
86	149.0
87	96.0
88	43.0
89	50.5
90	58.0
91	32.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1901208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.020037936066206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41711048835111	13.687022712205636
2	6.686404168452715	2.2760570520587287
3	2.3300789693388464	1.1897409735653164
4	1.206628242627327	0.82147433856984
5	0.7509452492114906	0.639055831474413
6	0.5205725242156465	0.5316098466374428
7	0.38732609924442996	0.4614613431938119
8	0.3101096396690528	0.4222462265205681
9	0.2533196641580087	0.38803592645287727
>10	3.7397888481977537	16.18704413602084
>50	1.8630142326052745	23.116206223286685
>100	1.5263392199230612	38.650177960224134
>500	0.007123742300768866	0.8061828903478251
>1k	9.291837783611564E-4	0.3972663481109795
>5k	3.097279261203855E-4	0.4264181913309068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8089	0.4254663350880072	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4747	0.24968335921161702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.259813760514368E-5	0.0	0.0	0.03739727583725715	0.0
2	5.259813760514368E-5	0.0	0.0	0.1261829321147397	0.0
3	5.259813760514368E-5	0.0	0.0	0.17562518146357473	0.0
4	5.259813760514368E-5	0.0	0.0	0.2534704251191874	0.0
5	5.259813760514368E-5	0.0	0.0	0.40926610870562297	0.0
6	5.259813760514368E-5	0.0	0.0	0.6017752923404488	0.0
7	5.259813760514368E-5	0.0	0.0	0.7118105962104094	0.0
8	5.259813760514368E-5	0.0	0.0	1.0240331410345422	0.0
9	1.0519627521028735E-4	0.0	0.0	1.1598415323310232	0.0
10	1.0519627521028735E-4	0.0	0.0	1.3899583843535268	0.0
11	1.0519627521028735E-4	0.0	0.0	1.5733154920450576	0.0
12	1.0519627521028735E-4	0.0	0.0	1.7424185044455947	0.0
13	1.0519627521028735E-4	0.0	0.0	1.8182124207346066	0.0
14	1.0519627521028735E-4	0.0	0.0	1.846562816903779	0.0
15	1.5779441281543104E-4	0.0	0.0	1.8866951958965037	0.0
16	1.5779441281543104E-4	0.0	0.0	1.9712204030279696	0.0
17	1.5779441281543104E-4	0.0	0.0	2.0679483780838286	0.0
18	1.5779441281543104E-4	0.0	0.0	2.237419577447602	5.259813760514368E-5
19	1.5779441281543104E-4	0.0	0.0	2.2985912114823837	5.259813760514368E-5
20	2.103925504205747E-4	0.0	0.0	2.3688623233228556	5.259813760514368E-5
21	2.103925504205747E-4	0.0	0.0	2.4540713062431885	5.259813760514368E-5
22	2.103925504205747E-4	0.0	0.0	2.538175728273813	5.259813760514368E-5
23	2.103925504205747E-4	0.0	0.0	2.6367972362834577	5.259813760514368E-5
24	2.103925504205747E-4	0.0	0.0	2.7161678259296194	5.259813760514368E-5
25	2.103925504205747E-4	0.0	0.0	2.779285591055792	5.259813760514368E-5
26	2.103925504205747E-4	0.0	0.0	2.8446650760989853	5.259813760514368E-5
27	2.103925504205747E-4	0.0	0.0	2.9132004493984875	5.259813760514368E-5
28	2.103925504205747E-4	0.0	0.0	2.9743194852956645	5.259813760514368E-5
29	2.103925504205747E-4	0.0	0.0	3.046957513328368	5.259813760514368E-5
30	2.103925504205747E-4	0.0	0.0	3.1495764797960035	5.259813760514368E-5
31	2.629906880257184E-4	0.0	0.0	3.238467332348696	5.259813760514368E-5
32	2.629906880257184E-4	0.0	0.0	3.318626894058935	5.259813760514368E-5
33	2.629906880257184E-4	0.0	0.0	3.3957357637880756	5.259813760514368E-5
34	3.155888256308621E-4	0.0	0.0	3.478840821204203	5.259813760514368E-5
35	3.155888256308621E-4	0.0	0.0	3.5895599008630303	5.259813760514368E-5
36	3.155888256308621E-4	0.0	0.0	3.67913452920459	5.259813760514368E-5
37	3.155888256308621E-4	0.0	0.0	3.772075438352879	5.259813760514368E-5
38	3.155888256308621E-4	0.0	0.0	3.8598617300158637	5.259813760514368E-5
39	3.155888256308621E-4	0.0	0.0	3.9464908626515354	5.259813760514368E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.034454E-4	45.0	1
GTATTCG	25	3.8917715E-5	45.0	34
TCGATAG	95	0.0	45.0	1
ACGGCTA	20	7.034454E-4	45.0	36
ACGTATG	50	2.1827873E-11	45.0	1
TAGTACT	20	7.034454E-4	45.0	43
TCAATCG	30	2.1662545E-6	44.999996	37
TACGTAA	30	2.1662545E-6	44.999996	19
AACGGGA	345	0.0	42.391304	4
CGGTCTA	140	0.0	41.785713	31
GTATACT	65	0.0	41.53846	37
ATTACGG	115	0.0	41.086956	2
GCGATAT	250	0.0	40.500004	9
GGTACCT	1340	0.0	40.46642	8
CATATGC	1325	0.0	40.075474	33
TAACGGG	540	0.0	40.000004	3
AGGGTAC	765	0.0	40.0	6
TAGCATA	1330	0.0	39.755642	30
GTTGATC	1355	0.0	39.686344	16
CGTTTTT	6050	0.0	39.681816	1
>>END_MODULE