##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545507_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1659039
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182969176734243	31.0	31.0	33.0	30.0	34.0
2	31.47275802437435	31.0	31.0	34.0	30.0	34.0
3	31.579757317338533	31.0	31.0	34.0	30.0	34.0
4	35.3627479522784	37.0	35.0	37.0	33.0	37.0
5	35.252317154690154	37.0	35.0	37.0	33.0	37.0
6	35.29797551474076	37.0	35.0	37.0	32.0	37.0
7	35.80086242698333	37.0	35.0	37.0	35.0	37.0
8	35.78732446916558	37.0	35.0	37.0	35.0	37.0
9	37.42952034280086	39.0	37.0	39.0	35.0	39.0
10	36.752535654677196	39.0	37.0	39.0	32.0	39.0
11	36.466014361326046	38.0	35.0	39.0	32.0	39.0
12	35.79130388134335	37.0	35.0	39.0	31.0	39.0
13	35.50623764721625	37.0	35.0	39.0	30.0	39.0
14	36.49738613739641	38.0	35.0	40.0	31.0	41.0
15	36.71813260568317	38.0	35.0	40.0	31.0	41.0
16	36.816300882619394	38.0	35.0	40.0	32.0	41.0
17	36.74306390627345	38.0	35.0	40.0	31.0	41.0
18	36.68744074129662	38.0	35.0	40.0	31.0	41.0
19	36.61318932225222	38.0	35.0	40.0	31.0	41.0
20	36.48982814749985	38.0	35.0	40.0	31.0	41.0
21	36.37234627998498	38.0	35.0	40.0	31.0	41.0
22	36.269968337091534	38.0	35.0	40.0	30.0	41.0
23	36.212115568109006	38.0	35.0	40.0	30.0	41.0
24	36.09687535977153	38.0	34.0	40.0	30.0	41.0
25	35.97545928697276	37.0	34.0	40.0	30.0	41.0
26	35.83303707748883	37.0	34.0	40.0	30.0	41.0
27	35.73432270127466	37.0	34.0	40.0	30.0	41.0
28	35.71742978917313	37.0	34.0	40.0	30.0	41.0
29	35.7248111708043	37.0	34.0	40.0	30.0	41.0
30	35.666901742514796	37.0	34.0	40.0	30.0	41.0
31	35.54585094141849	37.0	34.0	40.0	29.0	41.0
32	35.3944982607401	37.0	34.0	40.0	29.0	41.0
33	35.20903185518846	37.0	34.0	40.0	27.0	41.0
34	35.0579299220814	37.0	34.0	40.0	27.0	41.0
35	34.910990639761934	37.0	34.0	40.0	26.0	41.0
36	34.75793215228816	36.0	33.0	40.0	25.0	41.0
37	34.673204789037506	36.0	33.0	40.0	25.0	41.0
38	34.63070669224774	36.0	33.0	40.0	26.0	41.0
39	34.5542558071269	36.0	33.0	40.0	25.0	41.0
40	34.4032075195339	36.0	33.0	40.0	24.0	41.0
41	34.359279679380656	36.0	33.0	40.0	24.0	41.0
42	34.299510740856604	36.0	33.0	40.0	24.0	41.0
43	34.25967502873652	35.0	33.0	40.0	24.0	41.0
44	34.125458171869376	35.0	33.0	40.0	24.0	41.0
45	34.04518157800992	35.0	33.0	40.0	24.0	41.0
46	33.972466590598536	35.0	33.0	40.0	23.0	41.0
47	33.87787990517402	35.0	33.0	39.0	23.0	41.0
48	33.81230399044266	35.0	33.0	39.0	23.0	41.0
49	33.75856745983669	35.0	33.0	39.0	24.0	41.0
50	33.700152919853	35.0	33.0	39.0	24.0	41.0
51	33.55456924159107	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	5.0
14	17.0
15	42.0
16	148.0
17	386.0
18	900.0
19	1706.0
20	3035.0
21	4772.0
22	7089.0
23	10168.0
24	14641.0
25	20106.0
26	26428.0
27	31399.0
28	36191.0
29	41882.0
30	50673.0
31	61078.0
32	76647.0
33	99242.0
34	158384.0
35	213664.0
36	134268.0
37	163155.0
38	214087.0
39	288763.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.95576535572702	23.453939298593944	28.31096797603914	15.279327369639894
2	31.523912337202443	26.42023484679986	28.961826696057173	13.09402611994052
3	29.298346814029085	25.826276537200147	31.325544486898742	13.549832161872025
4	26.336391127634734	28.136469365699057	30.509168259456228	15.017971247209982
5	23.069499873119316	32.79850564091621	29.429808461404466	14.702186024560001
6	22.15740558238836	41.07528515001757	26.060086592298315	10.707222675295759
7	86.9769788413654	4.597420554911609	6.715273118956215	1.7103274847667838
8	87.71662390094507	3.852169840491996	6.424622929298226	2.006583329264713
9	81.98903099927126	6.140120877206623	8.439162671884146	3.431685451637966
10	46.28010553097305	26.77574186019738	14.319976805849652	12.624175802979916
11	40.901931780988875	22.53286390494738	20.91361324236501	15.651591071698737
12	37.00208373642814	20.745744976459264	25.940017082178297	16.312154204934302
13	22.963414362169907	33.540260355543175	26.116866450999645	17.37945883128727
14	18.77225309350775	35.832732081644856	27.86040593379661	17.53460889105078
15	18.364004703927996	22.64949769113324	41.21307576253482	17.77342184240395
16	19.94250888616844	19.936421024460547	39.81919653486145	20.30187355450957
17	19.62117828453701	20.00260391708694	28.61132257891466	31.764895219461387
18	23.12959490403782	22.08754586239383	32.74895888523417	22.033900348334186
19	29.5716978322993	23.27950096411236	25.82585460619069	21.32294659739765
20	30.79077707034012	22.604652452413717	25.835317915974247	20.769252561271916
21	24.926659349177445	25.953699702056433	27.60941725902767	21.510223689738456
22	24.72262556817531	23.625665219443302	25.20905174622176	26.442657466159623
23	22.326238262030007	27.98306730583187	25.222734366099893	24.46796006603823
24	21.86573070313597	23.405839163515747	35.49783941185228	19.230590721496
25	21.2272888099677	24.130656361905896	32.451798902858826	22.19025592526758
26	20.09356018755436	30.221049655854987	26.836921856568775	22.848468300021878
27	20.69559546219227	30.256190481356978	27.571443468176454	21.476770588274295
28	19.56741221876038	27.00816557055018	34.16098114631422	19.26344106437522
29	19.918579370346325	24.56735495669481	32.44414386883009	23.069921804128775
30	20.81789517907656	28.817767394256556	29.967107464019833	20.39722996264705
31	28.322360113294504	25.57745779333699	25.19548967806061	20.9046924153079
32	29.25121109268679	25.147992301567353	26.620893179726334	18.979903426019522
33	28.066006887119592	25.76135943760213	24.9621618298304	21.210471845447877
34	21.95921855966014	26.39063940027932	27.229016316072137	24.421125723988403
35	22.44703108245195	24.733836877855193	29.733779615789622	23.085352423903235
36	29.453979080660552	25.506332280314087	24.648064331218254	20.39162430780711
37	22.550524731486117	31.301735522793617	26.49690573880421	19.65083400691605
38	23.49715708913413	30.459862607208148	24.294184766000075	21.748795537657646
39	23.08028925178974	29.988686221360677	25.75714012750755	21.17388439934203
40	25.52339034826788	25.68902840740935	25.696261510428624	23.09131973389414
41	20.11773080681045	24.998869827653238	27.15632363072839	27.727075734807922
42	22.09037882774305	26.42348974315854	24.9171357635354	26.568995665563016
43	22.888310642486402	25.56968220759126	26.36634822930624	25.17565892061609
44	21.36501914662645	27.776562214631483	28.150694468303637	22.70772417043843
45	20.264442246384803	32.34945049513604	24.634020056189154	22.75208720229
46	22.7791510627538	30.457813228019354	25.553528277514875	21.209507431711973
47	22.38723743082592	26.99785839874771	26.834390270512028	23.78051389991435
48	23.10506262963077	24.619794953584577	29.941008017291935	22.33413439949272
49	22.41755618764839	25.041605411325474	30.327677649530845	22.21316075149529
50	21.917025458714352	30.31218675389789	26.57809732019561	21.19269046719215
51	21.010536822823333	30.758228106753364	25.316644153633522	22.914590916789777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1353.0
1	2093.5
2	2834.0
3	9000.0
4	15166.0
5	11058.5
6	6951.0
7	6990.5
8	7030.0
9	7349.5
10	7669.0
11	7853.0
12	8037.0
13	7669.5
14	7302.0
15	6996.5
16	6691.0
17	6332.5
18	5974.0
19	5926.0
20	5878.0
21	6378.0
22	6878.0
23	6759.5
24	6641.0
25	8107.5
26	11939.5
27	14305.0
28	17058.5
29	19812.0
30	21474.0
31	23136.0
32	26624.5
33	30113.0
34	35022.0
35	39931.0
36	41751.0
37	43571.0
38	50052.0
39	56533.0
40	75640.0
41	94747.0
42	112250.5
43	129754.0
44	131259.5
45	132765.0
46	131078.5
47	129392.0
48	124472.0
49	119552.0
50	114290.0
51	109028.0
52	106196.5
53	103365.0
54	94653.0
55	85941.0
56	85202.0
57	84463.0
58	81362.5
59	78262.0
60	72070.5
61	65879.0
62	61727.5
63	57576.0
64	50232.0
65	42888.0
66	37074.0
67	31260.0
68	25956.5
69	20653.0
70	19211.0
71	17769.0
72	15002.0
73	12235.0
74	9737.5
75	6050.0
76	4860.0
77	3910.5
78	2961.0
79	2382.0
80	1803.0
81	1381.0
82	959.0
83	551.5
84	144.0
85	115.0
86	86.0
87	64.5
88	43.0
89	31.0
90	19.0
91	11.0
92	3.0
93	4.0
94	5.0
95	6.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1659039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.186370834475717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1637224119502	13.433507191747967
2	5.969353635218351	2.051830504339765
3	2.168946288113201	1.1182894568271924
4	1.2195267015961526	0.8383695254470596
5	0.840666447580755	0.7224002658112099
6	0.6000232019721728	0.618733275502997
7	0.5063391686528489	0.6091492904341602
8	0.4165197594769371	0.5726770437005817
9	0.38507211464585267	0.5956192944287435
>10	6.209571823567812	26.85663114340411
>50	2.7329802654126882	33.356258377644714
>100	0.7809518392806172	17.774728913309367
>500	0.00456902516230723	0.4619683847177741
>1k	0.0014058538960945322	0.4629952495896625
>5k	3.5146347402363306E-4	0.5268420830947209
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8722	0.5257260377845246	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3391	0.2043954361530983	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1890	0.11392137255362893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014586757755544022	0.0
2	0.0	0.0	0.0	0.056840134559826505	0.0
3	0.0	0.0	0.0	0.08275875371223944	0.0
4	0.0	0.0	0.0	0.12055171698796713	0.0
5	0.0	0.0	0.0	0.18824150607671067	0.0
6	0.0	0.0	0.0	0.2922776378373263	0.0
7	0.0	0.0	0.0	0.34387377270817626	0.0
8	0.0	0.0	0.0	0.5058952803400041	0.0
9	0.0	0.0	0.0	0.5893773443541713	0.0
10	0.0	0.0	0.0	0.7391628527117204	0.0
11	0.0	0.0	0.0	0.850854018501072	0.0
12	0.0	0.0	0.0	0.9593505637902424	0.0
13	0.0	0.0	0.0	1.0072698712929593	0.0
14	0.0	0.0	0.0	1.026919801161998	0.0
15	0.0	0.0	0.0	1.052898696172905	0.0
16	0.0	0.0	0.0	1.1009385553926099	0.0
17	0.0	0.0	0.0	1.154885448744725	0.0
18	6.0275858493983565E-5	0.0	0.0	1.242647098711965	0.0
19	6.0275858493983565E-5	0.0	0.0	1.279596199968777	0.0
20	6.0275858493983565E-5	0.0	0.0	1.3211865423296258	0.0
21	6.0275858493983565E-5	0.0	0.0	1.3696483325587885	0.0
22	6.0275858493983565E-5	0.0	0.0	1.4212444674296385	0.0
23	6.0275858493983565E-5	0.0	0.0	1.4784462571404289	0.0
24	6.0275858493983565E-5	0.0	0.0	1.5219654269730851	0.0
25	6.0275858493983565E-5	0.0	0.0	1.5605419764092345	0.0
26	1.2055171698796713E-4	0.0	0.0	1.599118525845384	0.0
27	1.2055171698796713E-4	0.0	0.0	1.643059626687498	0.0
28	1.2055171698796713E-4	0.0	0.0	1.6847102449068407	0.0
29	1.2055171698796713E-4	0.0	0.0	1.727747207871545	0.0
30	1.2055171698796713E-4	0.0	0.0	1.7978480313000478	0.0
31	1.2055171698796713E-4	0.0	0.0	1.8476358904160781	0.0
32	1.2055171698796713E-4	0.0	0.0	1.8980867839755424	0.0
33	1.808275754819507E-4	0.0	0.0	1.9485979533935007	0.0
34	1.808275754819507E-4	0.0	0.0	1.9999529848303748	0.0
35	1.808275754819507E-4	0.0	0.0	2.064990636145383	0.0
36	1.808275754819507E-4	0.0	0.0	2.1186361502050284	0.0
37	1.808275754819507E-4	0.0	0.0	2.1770434570856985	0.0
38	1.808275754819507E-4	0.0	0.0	2.237982350023116	0.0
39	1.808275754819507E-4	0.0	0.0	2.296329381045292	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGT	25	3.891556E-5	45.000004	25
TCGAATA	20	7.034193E-4	45.0	1
TACGGGA	355	0.0	43.732395	4
CGATATC	90	0.0	42.5	10
ACGGGAT	340	0.0	40.367645	5
CGGGATA	145	0.0	40.344826	6
CGTTTTT	5710	0.0	40.074432	1
TATTACG	45	1.9292202E-8	40.0	1
CGCATCG	155	0.0	39.193546	21
TCGTTTG	75	0.0	39.0	1
GGTACCT	995	0.0	38.668343	8
TCGATAG	35	6.249964E-6	38.57143	1
CGGTCTA	140	0.0	38.57143	31
TAATCGC	35	6.249964E-6	38.57143	33
GCGATAC	130	0.0	38.076927	9
TCGTAAG	65	9.094947E-12	38.076927	1
TATGGGT	345	0.0	37.826084	4
AGGGATT	1285	0.0	37.82101	6
ATAGGGC	775	0.0	37.741936	4
CGCATTA	30	1.1401401E-4	37.500004	38
>>END_MODULE