FastQCFastQC Report
Thu 26 May 2016
SRR1545506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545506_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3382098
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272510.8057424710933865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3550.042.46478731
CGTTTTT165250.041.228441
TATGGGC12250.038.75514
GGACGTA1050.038.571438
GGCGATA17350.038.515858
GGGCGAT80500.038.4596257
GCTACGA3600.038.12510
TAGGGCG18650.038.1233255
ATAGGGC19150.038.067894
GTTGATC27250.037.89908216
TCACGAC4050.037.7777825
TATACGG2450.037.653062
TAGGGTA13850.037.5270735
CTAAGCG900.037.51
TATACGC301.140391E-437.49999615
CAATACG301.140391E-437.4999961
TAACGGG8650.037.196533
ATAGGGA37700.037.0623324
CATATGC26750.036.92523233
AGCATAT26800.036.85634231