##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545506_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3382098
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.037754376129847	31.0	31.0	33.0	30.0	34.0
2	31.322140872322446	31.0	31.0	34.0	30.0	34.0
3	31.410603714026028	31.0	31.0	34.0	30.0	34.0
4	35.21237232037629	37.0	35.0	37.0	32.0	37.0
5	35.08051333817057	37.0	35.0	37.0	32.0	37.0
6	35.12286840889885	37.0	35.0	37.0	32.0	37.0
7	35.716358603446736	37.0	35.0	37.0	35.0	37.0
8	35.69828698044823	37.0	35.0	37.0	35.0	37.0
9	37.32671909566192	39.0	37.0	39.0	35.0	39.0
10	36.56933713925498	39.0	35.0	39.0	32.0	39.0
11	36.2919312213898	38.0	35.0	39.0	31.0	39.0
12	35.82352728986564	37.0	35.0	39.0	30.0	39.0
13	35.64295091390019	37.0	35.0	39.0	30.0	39.0
14	36.657295560329715	38.0	35.0	40.0	31.0	41.0
15	36.81997180448349	38.0	35.0	41.0	31.0	41.0
16	36.86554824845407	38.0	35.0	41.0	31.0	41.0
17	36.7663621811077	38.0	35.0	40.0	31.0	41.0
18	36.65534558726566	38.0	35.0	40.0	31.0	41.0
19	36.58151242217109	38.0	35.0	40.0	31.0	41.0
20	36.46584486907239	38.0	35.0	40.0	30.0	41.0
21	36.349858874580214	38.0	35.0	40.0	30.0	41.0
22	36.246358325512745	38.0	35.0	40.0	30.0	41.0
23	36.13755426365528	38.0	34.0	40.0	30.0	41.0
24	36.024717497837145	38.0	34.0	40.0	30.0	41.0
25	35.9103648090623	38.0	34.0	40.0	30.0	41.0
26	35.734344776526285	38.0	34.0	40.0	29.0	41.0
27	35.63490265509752	37.0	34.0	40.0	29.0	41.0
28	35.59872629356098	37.0	34.0	40.0	29.0	41.0
29	35.547679280730485	37.0	34.0	40.0	29.0	41.0
30	35.447310515543904	37.0	34.0	40.0	28.0	41.0
31	35.3335074855903	37.0	34.0	40.0	27.0	41.0
32	35.19300120812584	37.0	34.0	40.0	27.0	41.0
33	34.9685183575402	37.0	33.0	40.0	26.0	41.0
34	34.774856316996136	37.0	33.0	40.0	25.0	41.0
35	34.60263185750383	37.0	33.0	40.0	24.0	41.0
36	34.467903650337746	37.0	33.0	40.0	24.0	41.0
37	34.42087219234924	37.0	33.0	40.0	24.0	41.0
38	34.34025536811766	37.0	33.0	40.0	23.0	41.0
39	34.253459834694326	37.0	33.0	40.0	23.0	41.0
40	34.102028385930865	36.0	33.0	40.0	23.0	41.0
41	33.9988252853702	36.0	33.0	40.0	23.0	41.0
42	33.937097624019174	36.0	33.0	40.0	23.0	41.0
43	33.874173368128304	36.0	33.0	40.0	23.0	41.0
44	33.749135891390495	36.0	33.0	40.0	23.0	41.0
45	33.65507504513471	35.0	32.0	40.0	23.0	41.0
46	33.57560721185489	35.0	32.0	40.0	23.0	41.0
47	33.46364712081081	35.0	32.0	39.0	22.0	41.0
48	33.34592049077229	35.0	32.0	39.0	22.0	41.0
49	33.28861197990123	35.0	32.0	39.0	22.0	41.0
50	33.24393024684678	35.0	32.0	39.0	22.0	41.0
51	33.078540598173085	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	14.0
14	56.0
15	163.0
16	474.0
17	1202.0
18	2592.0
19	4767.0
20	8296.0
21	12749.0
22	18437.0
23	26260.0
24	35775.0
25	48748.0
26	63658.0
27	75030.0
28	83679.0
29	94972.0
30	110711.0
31	131172.0
32	157440.0
33	194816.0
34	283994.0
35	359851.0
36	288163.0
37	343791.0
38	444846.0
39	590141.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.07068630181621	23.061366051486385	26.706381660141133	16.161565986556273
2	34.056464360287606	24.84239072906817	27.982630899518586	13.11851401112564
3	28.814274453312706	25.951199521716994	31.61141398031636	13.62311204465394
4	26.549171549730378	28.103413916450677	30.57466105358272	14.772753480236231
5	23.384360831649467	32.281471441690925	29.392377157610454	14.941790569049152
6	21.63180960457089	41.0786145167881	26.811257391122318	10.478318487518694
7	85.85658960798888	4.242662394762068	7.974251485320651	1.9264965119283946
8	85.67472024760961	3.9154986047122233	8.104821326880533	2.304959820797623
9	79.75951613465962	6.388904165402659	10.216587455478818	3.634992244458913
10	40.479637195610536	29.875864034690892	15.915387431115244	13.72911133858333
11	36.05974752949205	24.555202126017637	23.66684821078514	15.718202133705173
12	33.73261212419037	21.011809829283482	27.527883579955397	17.72769446657075
13	24.310708914998916	27.877607331307374	28.14202900093374	19.669654752759975
14	19.819827811021444	31.153887320828673	29.408609685467425	19.617675182682465
15	19.48988468104709	23.720306153162916	37.680694054400554	19.10911511138944
16	21.668147995711536	21.04876913679024	37.174676783463994	20.10840608403423
17	21.05914731033814	20.98803760269513	30.372804099703792	27.580010987262938
18	23.09462942824247	22.795968656141838	31.6099947429081	22.499407172707592
19	28.333241674250715	24.053087757953794	26.557657406733924	21.056013161061564
20	30.646036868239772	22.641508318209585	26.17224574805343	20.540209065497216
21	26.462479798042516	25.149152981374286	27.510350084474194	20.878017136109005
22	25.34959069784495	23.87659967274751	26.30881778115241	24.464991848255135
23	23.92949583365118	27.127629063380187	26.298528309942526	22.644346793026106
24	22.797713135456156	23.614986910491652	32.61543574432201	20.971864209730175
25	23.168370638579958	23.959772898360722	30.281381556655067	22.59047490640425
26	21.82183958004765	28.569456000387927	27.173044660444496	22.43565975911993
27	22.58769556647974	27.562891436025804	27.58246508528138	22.26694791221307
28	21.34802125781098	27.059801342243777	31.41981693020131	20.17236046974393
29	22.466350768073546	24.764924020534	30.960782330967344	21.80794288042511
30	22.79898453563439	26.130940025983872	29.256780850229653	21.81329458815209
31	26.093507639341023	26.09149705301266	25.981565288764546	21.83343001888177
32	28.25086676967965	25.314937651126606	26.475371204500874	19.958824374692867
33	26.829559640199662	25.69130167132945	26.152435559229804	21.326703129241082
34	22.91148866768497	26.919651648178146	27.556534435134637	22.612325249002247
35	23.89108772129016	24.781895734541102	28.590714994065813	22.736301550102926
36	26.338858306293904	27.5759898146062	25.0426510408628	21.042500838237093
37	23.560819349409744	29.357398868986056	27.112224424011366	19.969557357592834
38	23.88212878515052	29.268637396077818	24.730684918059737	22.118548900711925
39	23.85205869256302	28.155925700556285	25.685122075114325	22.306893531766377
40	24.661083150163005	25.425667736416862	27.86152264068043	22.051726472739702
41	20.665959413358216	26.000813696114065	28.529924325078692	24.80330256544902
42	23.23069881475936	26.946617158935076	25.129815871686745	24.692868154618818
43	22.92071371083866	26.393972025647983	26.95427512745048	23.731039136062883
44	22.921157222528738	27.404202953314776	27.323602095504036	22.35103772865245
45	21.643311341066994	28.768622316680357	25.64848800951362	23.93957833273903
46	22.421408250145326	29.019649933266273	25.819121740410832	22.739820076177566
47	23.65422291134083	27.52004820676397	26.449854498598206	22.375874383296996
48	23.460940516803475	25.208790519967195	28.228425078161546	23.101843885067787
49	22.47776380223163	26.570105301502206	28.349799444013748	22.602331452252418
50	21.644464471461205	28.603635967970177	26.975563688574372	22.776335871994245
51	20.656113453838415	28.80232920512652	26.827992565561377	23.713564775473685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3048.0
1	4506.0
2	5964.0
3	25455.0
4	44946.0
5	31588.5
6	18231.0
7	18143.5
8	18056.0
9	18545.0
10	19034.0
11	19053.0
12	19072.0
13	18575.5
14	18079.0
15	17643.5
16	17208.0
17	16237.0
18	15266.0
19	15330.5
20	15395.0
21	15649.0
22	15903.0
23	16264.0
24	16625.0
25	18272.5
26	22419.0
27	24918.0
28	30342.0
29	35766.0
30	44633.0
31	53500.0
32	56772.0
33	60044.0
34	69451.0
35	78858.0
36	85967.5
37	93077.0
38	103245.0
39	113413.0
40	138463.0
41	163513.0
42	187978.0
43	212443.0
44	226814.0
45	241185.0
46	244234.5
47	247284.0
48	239250.0
49	231216.0
50	217968.0
51	204720.0
52	197304.5
53	189889.0
54	186129.5
55	182370.0
56	181585.0
57	180800.0
58	183413.5
59	186027.0
60	177310.5
61	168594.0
62	151727.5
63	134861.0
64	116155.0
65	97449.0
66	81746.5
67	66044.0
68	59323.5
69	52603.0
70	46549.5
71	40496.0
72	35739.5
73	30983.0
74	25938.0
75	16587.5
76	12282.0
77	9486.5
78	6691.0
79	4973.5
80	3256.0
81	2300.5
82	1345.0
83	913.5
84	482.0
85	326.0
86	170.0
87	140.5
88	111.0
89	79.0
90	47.0
91	30.0
92	13.0
93	8.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3382098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.054800964584935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31513468800281	14.320189702711373
2	5.888212654334569	2.12621015022322
3	2.245015299299909	1.215999131739238
4	1.2808026846134042	0.9249855018240426
5	0.9335194965915107	0.8427254353759626
6	0.6837259273094282	0.7406721319138797
7	0.5448849323066481	0.6886452300978508
8	0.41018717557057044	0.5924678250521525
9	0.3589688398450545	0.5832999860301383
>10	5.142264157422586	23.309552285680592
>50	2.2351428117585663	29.093470563371493
>100	0.9537252730954938	23.359933036700838
>500	0.005775727347765269	0.6813629566439247
>1k	0.0024753117204708297	0.7085650371641006
>5k	0.0	0.0
>10k+	1.6502078136472196E-4	0.8119210254713188
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27251	0.8057424710933865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9134892010816956E-5	0.0	0.0	0.018420518861369483	0.0
2	5.9134892010816956E-5	0.0	0.0	0.062269041287390255	0.0
3	5.9134892010816956E-5	0.0	0.0	0.10390000526300538	0.0
4	8.870233801622543E-5	0.0	0.0	0.1555839008804594	0.0
5	8.870233801622543E-5	0.0	0.0	0.2564975940969185	0.0
6	8.870233801622543E-5	0.0	0.0	0.4750305875228926	0.0
7	8.870233801622543E-5	0.0	0.0	0.5942465298166996	0.0
8	8.870233801622543E-5	0.0	0.0	0.9205824313783929	0.0
9	8.870233801622543E-5	0.0	0.0	1.095326037270357	0.0
10	8.870233801622543E-5	0.0	0.0	1.3417706997254366	0.0
11	1.1826978402163391E-4	0.0	0.0	1.4951961770475013	0.0
12	1.1826978402163391E-4	0.0	0.0	1.6260616930674392	0.0
13	1.478372300270424E-4	0.0	0.0	1.6907848323732784	0.0
14	1.478372300270424E-4	0.0	0.0	1.7289268377202553	0.0
15	1.478372300270424E-4	0.0	0.0	1.762929400626475	0.0
16	1.478372300270424E-4	0.0	0.0	1.8235426649375623	0.0
17	1.7740467603245087E-4	0.0	0.0	1.8869057017271529	0.0
18	1.7740467603245087E-4	0.0	0.0	1.9897412789339635	5.9134892010816956E-5
19	1.7740467603245087E-4	0.0	0.0	2.037906648476774	5.9134892010816956E-5
20	1.7740467603245087E-4	0.0	0.0	2.0922220467887094	5.9134892010816956E-5
21	1.7740467603245087E-4	0.0	0.0	2.1571521582165865	5.9134892010816956E-5
22	1.7740467603245087E-4	0.0	0.0	2.2246842048929394	5.9134892010816956E-5
23	1.7740467603245087E-4	0.0	0.0	2.30002205731472	5.9134892010816956E-5
24	1.7740467603245087E-4	0.0	0.0	2.364538224498521	5.9134892010816956E-5
25	1.7740467603245087E-4	0.0	0.0	2.419444971730565	5.9134892010816956E-5
26	1.7740467603245087E-4	0.0	0.0	2.476480575074998	5.9134892010816956E-5
27	1.7740467603245087E-4	0.0	0.0	2.5354676298557877	5.9134892010816956E-5
28	1.7740467603245087E-4	0.0	0.0	2.59534170801674	5.9134892010816956E-5
29	1.7740467603245087E-4	0.0	0.0	2.6599465775385576	5.9134892010816956E-5
30	1.7740467603245087E-4	0.0	0.0	2.7557155351500753	5.9134892010816956E-5
31	1.7740467603245087E-4	0.0	0.0	2.831881276060008	5.9134892010816956E-5
32	1.7740467603245087E-4	0.0	0.0	2.9122160268567026	5.9134892010816956E-5
33	1.7740467603245087E-4	0.0	0.0	2.9879973909685646	5.9134892010816956E-5
34	1.7740467603245087E-4	0.0	0.0	3.068243439427243	5.9134892010816956E-5
35	1.7740467603245087E-4	0.0	0.0	3.1520967162985816	5.9134892010816956E-5
36	1.7740467603245087E-4	0.0	0.0	3.235979560615925	5.9134892010816956E-5
37	1.7740467603245087E-4	0.0	0.0	3.324060982266037	5.9134892010816956E-5
38	1.7740467603245087E-4	0.0	0.0	3.4175236790891335	5.9134892010816956E-5
39	1.7740467603245087E-4	0.0	0.0	3.510217622316089	5.9134892010816956E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	355	0.0	42.464787	31
CGTTTTT	16525	0.0	41.22844	1
TATGGGC	1225	0.0	38.7551	4
GGACGTA	105	0.0	38.57143	8
GGCGATA	1735	0.0	38.51585	8
GGGCGAT	8050	0.0	38.459625	7
GCTACGA	360	0.0	38.125	10
TAGGGCG	1865	0.0	38.123325	5
ATAGGGC	1915	0.0	38.06789	4
GTTGATC	2725	0.0	37.899082	16
TCACGAC	405	0.0	37.77778	25
TATACGG	245	0.0	37.65306	2
TAGGGTA	1385	0.0	37.527073	5
CTAAGCG	90	0.0	37.5	1
TATACGC	30	1.140391E-4	37.499996	15
CAATACG	30	1.140391E-4	37.499996	1
TAACGGG	865	0.0	37.19653	3
ATAGGGA	3770	0.0	37.062332	4
CATATGC	2675	0.0	36.925232	33
AGCATAT	2680	0.0	36.856342	31
>>END_MODULE