FastQCFastQC Report
Thu 26 May 2016
SRR1545505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545505_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7635254
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322610.42252687336924216No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC138200.1810024918620913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA406.8230293E-945.041
CGTTTTT260900.042.542161
CGCGGAC700.041.7857174
GCGTAAG5650.040.619471
GTTAGCG3350.040.2985081
GGCGATA37300.039.5710458
CTAGCGG8650.038.757232
CGACGGT5300.038.63207628
AGGGCGA83750.038.6059726
GGACCGA37100.038.4501348
CGGTCTA5450.038.39449331
CGTAAGG14750.038.2881362
ACGTCGC1000.038.2520
CGGGATA25650.038.1578946
ACGGGAT49950.037.9279255
TCGATAG4350.037.758621
GGCGTAG4950.037.7272721
ACGGGAC27450.037.7049185
AAGGGAT161050.037.6234055
GGGCGAT160700.037.621347
Produced by FastQC (version 0.11.5)