##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545505_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7635254
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.025783818062894	31.0	31.0	33.0	28.0	34.0
2	31.304943882678952	31.0	31.0	34.0	28.0	34.0
3	31.42259877667462	31.0	31.0	34.0	28.0	34.0
4	35.187793752506465	37.0	35.0	37.0	32.0	37.0
5	35.03951944493268	37.0	35.0	37.0	32.0	37.0
6	35.096319650924514	37.0	35.0	37.0	32.0	37.0
7	35.76883283778117	37.0	35.0	37.0	35.0	37.0
8	35.783060524247134	37.0	35.0	37.0	35.0	37.0
9	37.43909973394467	39.0	37.0	39.0	35.0	39.0
10	36.569866961858764	39.0	35.0	39.0	32.0	39.0
11	36.19198680227272	38.0	35.0	39.0	31.0	39.0
12	35.75869028063769	37.0	35.0	39.0	30.0	39.0
13	35.551420817172556	37.0	35.0	39.0	30.0	39.0
14	36.52259047308708	38.0	35.0	40.0	30.0	41.0
15	36.75603326359542	38.0	35.0	40.0	31.0	41.0
16	36.83819071376014	38.0	35.0	40.0	31.0	41.0
17	36.77329202145731	38.0	35.0	40.0	31.0	41.0
18	36.66488017294513	38.0	35.0	40.0	31.0	41.0
19	36.64208158628384	38.0	35.0	40.0	31.0	41.0
20	36.54715599507233	38.0	35.0	40.0	31.0	41.0
21	36.436510298151184	38.0	35.0	40.0	30.0	41.0
22	36.353228065497234	38.0	35.0	40.0	30.0	41.0
23	36.2780127550439	38.0	34.0	40.0	30.0	41.0
24	36.21307058023217	38.0	34.0	40.0	30.0	41.0
25	36.12184178810554	38.0	34.0	40.0	30.0	41.0
26	35.99756144327353	38.0	34.0	40.0	30.0	41.0
27	35.91386769844199	38.0	34.0	40.0	30.0	41.0
28	35.917305305101834	38.0	34.0	40.0	30.0	41.0
29	35.93011457117209	38.0	34.0	40.0	30.0	41.0
30	35.8382369466687	38.0	34.0	40.0	29.0	41.0
31	35.72675041328029	38.0	34.0	40.0	29.0	41.0
32	35.59492100721207	38.0	34.0	40.0	29.0	41.0
33	35.434014375946106	38.0	34.0	40.0	27.0	41.0
34	35.32073156963737	38.0	34.0	40.0	27.0	41.0
35	35.17631725152824	38.0	34.0	40.0	27.0	41.0
36	35.042713313794145	38.0	34.0	40.0	25.0	41.0
37	35.023558613767136	38.0	34.0	40.0	26.0	41.0
38	34.973026437627354	38.0	34.0	40.0	26.0	41.0
39	34.92988118011529	37.0	34.0	40.0	25.0	41.0
40	34.842438902491	37.0	34.0	40.0	25.0	41.0
41	34.789874835860076	37.0	34.0	40.0	25.0	41.0
42	34.727283990814186	37.0	33.0	40.0	25.0	41.0
43	34.689844371909565	37.0	33.0	40.0	25.0	41.0
44	34.59512872787205	37.0	33.0	40.0	24.0	41.0
45	34.525057581581436	37.0	33.0	40.0	24.0	41.0
46	34.4768640309805	37.0	33.0	40.0	24.0	41.0
47	34.400749732752836	37.0	33.0	40.0	24.0	41.0
48	34.34753853637351	37.0	33.0	40.0	24.0	41.0
49	34.32247073902191	36.0	33.0	40.0	24.0	41.0
50	34.27014150413333	36.0	33.0	40.0	24.0	41.0
51	34.11634793027187	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	5.0
13	19.0
14	76.0
15	239.0
16	794.0
17	2030.0
18	4483.0
19	8579.0
20	14359.0
21	22361.0
22	33224.0
23	47915.0
24	67123.0
25	93176.0
26	121160.0
27	143597.0
28	165000.0
29	191791.0
30	230950.0
31	282248.0
32	346769.0
33	431962.0
34	638365.0
35	841642.0
36	602472.0
37	732101.0
38	1022916.0
39	1589154.0
40	738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.459810767264585	23.933755183521072	29.15469478814981	14.45173926106453
2	29.79314113191257	26.099563943779735	30.664480841108887	13.442814083198803
3	28.171348327115247	25.831190422741667	32.336854805354214	13.66060644478887
4	24.732720090255018	28.747753512849737	31.640073794532576	14.879452602362672
5	22.278630154281704	33.57150921239817	30.26982468428686	13.880035949033262
6	20.916448883036505	41.54515357314897	27.116648640634615	10.421748903179907
7	88.7545849817177	3.503445988830234	6.184456993834128	1.5575120356179375
8	89.78978302490003	2.6487789404255575	5.830663917664036	1.7307741170103834
9	83.97232102560046	5.644579735002922	7.639614870703712	2.743484368692908
10	39.883100156196505	36.30756750201107	13.233063366326778	10.57626897546565
11	30.939625584165242	23.055618058024002	30.00201434032188	16.002742017488874
12	30.43436406961707	23.14175795592393	29.912626351395776	16.511251623063227
13	21.56389296282743	32.81191431221542	30.150955030441683	15.473237694515468
14	16.879097931778038	35.505983167030195	30.336567716018354	17.278351185173406
15	15.495358766060697	26.414668064742834	41.88215611425632	16.20781705494015
16	18.450794171353042	23.8433194232962	40.058562557316364	17.647323848034393
17	19.14128855438208	23.09948038401866	30.706771510155395	27.05245955144387
18	20.500942077369004	24.50958409504124	35.208298243909105	19.781175583680646
19	25.316066760843842	25.248105694977536	29.75031348007545	19.68551406410317
20	26.69780206395229	25.46179865136117	29.38665825655571	18.45374102813083
21	22.33138543917465	28.132567691919615	30.59547200394381	18.940574864961928
22	21.85205626427097	25.64335122315512	29.424011303356775	23.080581209217137
23	18.26532555432995	30.164667737314304	29.606991463545285	21.963015244810453
24	18.671153572625087	25.516413730309427	37.76767609826733	18.044756598798152
25	17.57193670308807	28.161735025449055	34.20876371630859	20.05756455515429
26	17.663289263199363	32.93035176039985	29.539436409057252	19.866922567343536
27	17.766036336184758	32.44195936376183	30.502587078308068	19.289417221745342
28	16.209166060487313	28.81578006442222	36.12046174233365	18.854592132756814
29	17.718113896407374	25.345535328621683	36.062572378076744	20.873778396894195
30	18.506614187294883	29.84830629079268	32.025365495371865	19.61971402654057
31	23.476350623044105	27.76571676593863	29.40908056234933	19.34885204866793
32	24.287102956889186	27.386096127253918	29.819296122958054	18.507504792898835
33	23.436351953713654	28.31205091539849	28.694657702284694	19.55693942860316
34	19.309835664930073	27.395958274603572	30.69418515742895	22.60002090303741
35	19.08968581791778	27.00419658599439	32.46368752107003	21.442430075017803
36	24.462381998031763	27.103250265151623	28.959730743731644	19.47463699308497
37	19.488428282805	30.79140523681334	30.596414998112703	19.123751482268958
38	18.86506984574449	31.377960707004636	29.29683806196886	20.46013138528201
39	18.943233584632548	30.862064313773974	30.071416091723997	20.12328600986948
40	22.32029216054895	27.910872382241635	28.591871337875595	21.176964119333817
41	18.208942361315025	26.807346553238435	29.976684469174174	25.00702661627236
42	19.714799271903725	27.514736248460103	28.90429054488561	23.866173934750567
43	20.17408982071847	27.95288801132222	29.211470895401774	22.661551272557535
44	19.208712637457772	29.38677613082682	30.282830145532813	21.121681086182594
45	17.7107402058923	33.046222168902304	27.78000574702557	21.463031878179823
46	19.98883075795514	31.64820711923926	28.705672398062987	19.657289724742622
47	19.28789795336213	28.20054185492716	30.20584514935587	22.305715042354844
48	20.139120453622105	26.392717256033656	32.46147410420138	21.006688186142856
49	19.817795190572575	26.251451490677326	32.98469179938218	20.946061519367923
50	19.187259520115507	31.22680660001619	29.451305745689666	20.13462813417864
51	17.996742479032132	31.549428480047947	28.454338257771123	21.999490783148797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4920.0
1	6612.0
2	8304.0
3	33168.5
4	58033.0
5	43065.0
6	28097.0
7	29234.5
8	30372.0
9	31950.0
10	33528.0
11	33587.0
12	33646.0
13	33529.5
14	33413.0
15	32933.5
16	32454.0
17	31952.0
18	31450.0
19	33575.5
20	35701.0
21	37325.5
22	38950.0
23	43827.0
24	48704.0
25	64358.5
26	93867.0
27	107721.0
28	130613.5
29	153506.0
30	174209.5
31	194913.0
32	229035.0
33	263157.0
34	297889.5
35	332622.0
36	355573.0
37	378524.0
38	395711.0
39	412898.0
40	486344.0
41	559790.0
42	631859.5
43	703929.0
44	703405.0
45	702881.0
46	685446.5
47	668012.0
48	640061.5
49	612111.0
50	564542.5
51	516974.0
52	464867.0
53	412760.0
54	366742.5
55	320725.0
56	283277.5
57	245830.0
58	212081.5
59	178333.0
60	159659.5
61	140986.0
62	117771.5
63	94557.0
64	75450.5
65	56344.0
66	44031.5
67	31719.0
68	25549.0
69	19379.0
70	16514.5
71	13650.0
72	10563.5
73	7477.0
74	5721.0
75	2946.5
76	1928.0
77	1542.5
78	1157.0
79	879.5
80	602.0
81	689.5
82	777.0
83	552.5
84	328.0
85	202.5
86	77.0
87	47.5
88	18.0
89	14.5
90	11.0
91	7.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7635254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.325103597758748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82080034124664	11.434412341443748
2	8.538296264573134	2.4462395707673332
3	2.898118173478971	1.2454752922090087
4	1.4126278753190047	0.8094416263610629
5	0.8471221544370797	0.6067556311133876
6	0.588902841373535	0.5061656527014224
7	0.4630053099551276	0.4642819321993721
8	0.3331203167867168	0.3817586438790344
9	0.28547252765867787	0.3680481179722149
>10	2.3953189157331285	7.230742987227602
>50	0.6327670858206516	6.603484827522362
>100	1.695674386303826	59.25984722045925
>500	0.0841645753661713	7.1017968569457075
>1k	0.0044248626696781765	0.9330245786231475
>5k	0.0	0.0
>10k+	1.8436927790325733E-4	0.6085247205753851
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32261	0.42252687336924216	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13820	0.1810024918620913	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043613480311198556	0.0
2	0.0	0.0	0.0	0.15973273449711037	0.0
3	1.309714123459416E-5	0.0	0.0	0.22420996079501743	0.0
4	1.309714123459416E-5	0.0	0.0	0.3260795253176908	0.0
5	1.309714123459416E-5	0.0	0.0	0.5413179443670112	0.0
6	1.309714123459416E-5	0.0	0.0	0.814406436249534	0.0
7	1.309714123459416E-5	0.0	0.0	0.9798626214661621	0.0
8	1.309714123459416E-5	0.0	0.0	1.4085189569331944	0.0
9	1.309714123459416E-5	0.0	0.0	1.5828419067656427	0.0
10	1.309714123459416E-5	0.0	0.0	1.8620205693222518	0.0
11	3.929142370378248E-5	0.0	0.0	2.0873830785459133	0.0
12	3.929142370378248E-5	0.0	0.0	2.297526709654977	0.0
13	5.238856493837664E-5	0.0	0.0	2.3847274759949046	0.0
14	5.238856493837664E-5	0.0	0.0	2.418020409013243	0.0
15	5.238856493837664E-5	0.0	0.0	2.4672787571965515	0.0
16	5.238856493837664E-5	0.0	0.0	2.575696892336522	0.0
17	5.238856493837664E-5	0.0	0.0	2.7131906810172914	0.0
18	5.238856493837664E-5	0.0	0.0	2.9034397545910062	0.0
19	5.238856493837664E-5	0.0	0.0	2.9883092297911764	0.0
20	6.54857061729708E-5	0.0	0.0	3.08145609825161	0.0
21	7.858284740756496E-5	0.0	0.0	3.1990684265382656	0.0
22	9.167998864215912E-5	0.0	0.0	3.3341261469494006	0.0
23	9.167998864215912E-5	0.0	0.0	3.482320300018834	0.0
24	1.0477712987675328E-4	0.0	0.0	3.59828238850993	0.0
25	1.0477712987675328E-4	0.0	0.0	3.695292913634569	0.0
26	1.0477712987675328E-4	0.0	0.0	3.791700970262417	0.0
27	1.309714123459416E-4	0.0	0.0	3.8929549691470644	0.0
28	1.4406855358053576E-4	0.0	0.0	3.9956496535675172	0.0
29	1.4406855358053576E-4	0.0	0.0	4.115973090089733	0.0
30	1.8335997728431824E-4	0.0	0.0	4.274566373299434	0.0
31	1.964571185189124E-4	0.0	0.0	4.406506974096736	0.0
32	1.964571185189124E-4	0.0	0.0	4.524577702326602	0.0
33	2.2265140098810072E-4	0.0	0.0	4.644652293165362	0.0
34	2.4884568345728906E-4	0.0	0.0	4.770974220373022	0.0
35	2.4884568345728906E-4	0.0	0.0	4.923398750061229	0.0
36	2.619428246918832E-4	0.0	0.0	5.058875578991871	0.0
37	2.619428246918832E-4	0.0	0.0	5.192545002432139	0.0
38	2.7503996592647735E-4	0.0	0.0	5.334308459155387	0.0
39	2.7503996592647735E-4	0.0	0.0	5.4895750684915	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	40	6.8230293E-9	45.0	41
CGTTTTT	26090	0.0	42.54216	1
CGCGGAC	70	0.0	41.785717	4
GCGTAAG	565	0.0	40.61947	1
GTTAGCG	335	0.0	40.298508	1
GGCGATA	3730	0.0	39.571045	8
CTAGCGG	865	0.0	38.75723	2
CGACGGT	530	0.0	38.632076	28
AGGGCGA	8375	0.0	38.605972	6
GGACCGA	3710	0.0	38.450134	8
CGGTCTA	545	0.0	38.394493	31
CGTAAGG	1475	0.0	38.288136	2
ACGTCGC	100	0.0	38.25	20
CGGGATA	2565	0.0	38.157894	6
ACGGGAT	4995	0.0	37.927925	5
TCGATAG	435	0.0	37.75862	1
GGCGTAG	495	0.0	37.727272	1
ACGGGAC	2745	0.0	37.704918	5
AAGGGAT	16105	0.0	37.623405	5
GGGCGAT	16070	0.0	37.62134	7
>>END_MODULE