FastQCFastQC Report
Thu 26 May 2016
SRR1545503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545503_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4316078
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208170.4823128775707946No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC65870.15261540685780006No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT47150.10924269672605547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.035448E-445.00000433
TAATGCG950.042.631581
TAGTACG1350.041.6666641
CGTTTTT149200.041.3354571
AAATGCG2850.041.052631
CGGTCTA3500.039.85714331
CGTAAGG8000.039.3750042
GCTACGA6000.039.37500410
GCGTAAG4100.038.9634131
CGAATAT5850.038.84615714
TAGGGAC32150.038.70145
TAGGGCG19300.038.588085
CGCGAAA356.252292E-638.57142614
TAAGGGA41800.038.002394
TCGTAAG2250.038.01
CGAGGGA23450.037.8997884
TACGGGA13800.037.8260884
CATACGA13550.037.69372618
ATAGGGC20250.037.6666684
GGGCGAT95100.037.6182987
Produced by FastQC (version 0.11.5)