##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545503_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4316078
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.096639588070467	31.0	31.0	33.0	30.0	34.0
2	31.378435931880748	31.0	31.0	34.0	30.0	34.0
3	31.489304410161264	31.0	31.0	34.0	30.0	34.0
4	35.260243211545294	37.0	35.0	37.0	33.0	37.0
5	35.129177924958725	37.0	35.0	37.0	32.0	37.0
6	35.17558487126507	37.0	35.0	37.0	32.0	37.0
7	35.75584593234877	37.0	35.0	37.0	35.0	37.0
8	35.74760743434201	37.0	35.0	37.0	35.0	37.0
9	37.406051744199246	39.0	37.0	39.0	35.0	39.0
10	36.652818832282456	39.0	35.0	39.0	32.0	39.0
11	36.32955335839621	38.0	35.0	39.0	32.0	39.0
12	35.61952286311786	37.0	35.0	39.0	30.0	39.0
13	35.292365661602965	37.0	35.0	39.0	30.0	39.0
14	36.24560839725325	38.0	35.0	40.0	30.0	41.0
15	36.511219445061	38.0	35.0	40.0	31.0	41.0
16	36.62772591227499	38.0	35.0	40.0	31.0	41.0
17	36.553778916877775	38.0	35.0	40.0	31.0	41.0
18	36.481780681442736	38.0	35.0	40.0	31.0	41.0
19	36.42011173106695	38.0	35.0	40.0	31.0	41.0
20	36.318917313357176	38.0	35.0	40.0	31.0	41.0
21	36.1750475779168	38.0	34.0	40.0	30.0	41.0
22	36.07466130130178	38.0	34.0	40.0	30.0	41.0
23	36.00304721091695	37.0	34.0	40.0	30.0	41.0
24	35.915620848372065	37.0	34.0	40.0	30.0	41.0
25	35.796755990044666	37.0	34.0	40.0	30.0	41.0
26	35.64725846011124	37.0	34.0	40.0	29.0	41.0
27	35.53899605150787	37.0	34.0	40.0	29.0	41.0
28	35.53669998549609	37.0	34.0	40.0	29.0	41.0
29	35.552928607870385	37.0	34.0	40.0	29.0	41.0
30	35.48266574422427	37.0	34.0	40.0	29.0	41.0
31	35.354056391010545	37.0	34.0	40.0	29.0	41.0
32	35.20979370623052	37.0	34.0	40.0	28.0	41.0
33	35.02566033329333	37.0	34.0	40.0	27.0	41.0
34	34.8818538497219	37.0	34.0	40.0	26.0	41.0
35	34.75277995439378	36.0	33.0	40.0	26.0	41.0
36	34.57799071286478	36.0	33.0	40.0	24.0	41.0
37	34.51057464670472	36.0	33.0	40.0	24.0	41.0
38	34.4600996552889	36.0	33.0	40.0	24.0	41.0
39	34.411477966802266	36.0	33.0	40.0	24.0	41.0
40	34.271694348433925	36.0	33.0	40.0	24.0	41.0
41	34.20006635653943	36.0	33.0	40.0	23.0	41.0
42	34.16091901026812	36.0	33.0	40.0	23.0	41.0
43	34.09493317775999	35.0	33.0	40.0	23.0	41.0
44	33.980208652392285	35.0	33.0	40.0	23.0	41.0
45	33.90963207801156	35.0	33.0	40.0	23.0	41.0
46	33.85487866530679	35.0	33.0	40.0	23.0	41.0
47	33.75393169446891	35.0	33.0	39.0	23.0	41.0
48	33.67717010674969	35.0	33.0	39.0	23.0	41.0
49	33.65141709672531	35.0	32.0	39.0	24.0	41.0
50	33.5753860333386	35.0	32.0	39.0	23.0	41.0
51	33.42039022464376	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	9.0
13	19.0
14	61.0
15	157.0
16	501.0
17	1367.0
18	2727.0
19	5660.0
20	9416.0
21	14591.0
22	21782.0
23	30801.0
24	42903.0
25	58025.0
26	75029.0
27	88986.0
28	100806.0
29	116120.0
30	137714.0
31	166777.0
32	204540.0
33	258383.0
34	403596.0
35	545787.0
36	333868.0
37	402467.0
38	533160.0
39	760397.0
40	421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42286677858927	24.645453580774028	27.17580636865228	14.755873271984427
2	30.812001080610685	26.567962858873262	29.3355680782414	13.284467982274647
3	29.451112792678906	25.81037692089902	31.052705720332206	13.685804566089862
4	26.08729499327862	28.519340938694803	30.10089252325838	15.29247154476819
5	23.352983889540457	32.792178454606244	29.337259428583078	14.517578227270219
6	21.829030893324912	41.76138614733098	26.086507241064687	10.32307571827942
7	87.35660013558606	4.556660004754316	6.56779140692082	1.518948452738806
8	88.04678692090366	3.631746228867968	6.37150209055536	1.949964759673018
9	82.4505256855877	6.053713579782386	8.15963010863103	3.3361306259988814
10	45.7816564019464	29.27926696412808	13.289634710030725	11.649441923894795
11	40.8574173126621	22.754662913876906	21.53800278864284	14.849916984818162
12	37.021203045913445	21.29002302553383	25.766587165477546	15.92218676307518
13	23.14059199115493	34.36592202457879	25.765034830232448	16.728451154033824
14	18.18750726933109	37.35002008768146	27.561758615113074	16.90071402787438
15	17.376493195906097	23.969654857952058	41.80047255865163	16.853379387490218
16	19.70863362524959	19.771676971546853	40.94805515562972	19.571634247573837
17	19.63196679948787	20.180311847932312	29.170742512067672	31.01697884051215
18	22.309953619929946	22.609994536706704	33.46966852776988	21.610383315593463
19	28.9136109217674	24.136496143026147	25.962343590639463	20.98754934456699
20	30.920595040219386	23.101853117575725	25.41330346671214	20.56424837549275
21	24.862711007539716	26.390000366073085	27.808834780094337	20.938453846292862
22	24.47967807810702	23.8821448546574	25.587860089646203	26.05031697758938
23	21.68971459737289	28.890349062273668	25.805696745980956	23.61423959437248
24	21.03833619318279	23.97961760654001	35.169359775240395	19.812686425036805
25	19.93133580996451	24.99041954292763	33.64830292687018	21.42994172023768
26	19.437206649184745	31.305666857735194	27.444175012592453	21.812951480487612
27	20.385776160671796	30.820457832319065	27.92625619833562	20.867509808673525
28	18.397721264536923	28.76745971690039	33.80937508543636	19.025443933126322
29	19.38148476464049	24.486026434183998	33.66090696229308	22.47158183888243
30	20.141665651084157	29.019540425358393	29.82119414894726	21.01759977461019
31	27.63520492447078	26.466736699383098	25.274913938070625	20.623144438075496
32	28.80965079871124	26.11769296106326	26.580103510640914	18.49255272958459
33	27.713400916294837	26.577137855247283	25.014191124442142	20.695270104015727
34	21.0470246367188	27.697576364467924	27.480203091788425	23.775195907024848
35	22.06009715301716	25.599282496748206	29.15334245581289	23.18727789442174
36	28.756547031819164	25.977519405349025	25.396019256371176	19.869914306460636
37	22.61574049403185	31.51625155986523	26.541758513168666	19.326249432934254
38	22.251822140378373	31.652625369606387	24.033092080356287	22.062460409658954
39	22.32777072147445	30.123853183376205	25.943460706687876	21.604915388461468
40	24.81208634320325	26.197325442218606	26.015794895272975	22.974793319305164
41	19.207322017813393	25.72543869689102	27.466255243765286	27.600984041530296
42	22.0904951208018	26.23627283844268	25.23807493747796	26.435157103277557
43	22.073859647578196	26.010118445496122	27.21644511521803	24.699576791707656
44	20.91512247925084	28.66438002278921	28.03276493149568	22.387732566464276
45	19.9947962015515	32.63365490614395	24.565311377597904	22.80623751470664
46	22.36127335974929	31.28569502219376	25.583040899631566	20.769990718425387
47	22.33043517749216	27.774382205326226	26.71070819387416	23.184474423307456
48	23.069508938439018	25.299797640357752	29.479495041563197	22.151198379640032
49	22.46882470613367	25.457278575595716	30.303414349787005	21.77048236848361
50	21.168825030502227	31.21083539268753	26.693261799253857	20.927077777556384
51	20.18218855173609	31.575379314275597	25.22218087810276	23.02025125588555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4033.0
1	5841.5
2	7650.0
3	21897.5
4	36145.0
5	26489.0
6	16833.0
7	17534.0
8	18235.0
9	18808.5
10	19382.0
11	19917.0
12	20452.0
13	19653.0
14	18854.0
15	18191.0
16	17528.0
17	16900.0
18	16272.0
19	17630.0
20	18988.0
21	19984.5
22	20981.0
23	21365.5
24	21750.0
25	22479.0
26	27223.5
27	31239.0
28	40077.0
29	48915.0
30	57235.0
31	65555.0
32	76216.0
33	86877.0
34	96440.5
35	106004.0
36	117881.0
37	129758.0
38	145516.5
39	161275.0
40	217296.0
41	273317.0
42	316285.5
43	359254.0
44	367747.5
45	376241.0
46	363578.5
47	350916.0
48	335782.5
49	320649.0
50	297898.5
51	275148.0
52	271035.5
53	266923.0
54	244873.0
55	222823.0
56	211431.0
57	200039.0
58	190944.5
59	181850.0
60	172326.5
61	162803.0
62	147407.5
63	132012.0
64	111712.0
65	91412.0
66	79090.0
67	66768.0
68	57202.5
69	47637.0
70	40700.5
71	33764.0
72	29217.0
73	24670.0
74	20269.5
75	13402.5
76	10936.0
77	8925.0
78	6914.0
79	5376.0
80	3838.0
81	2663.5
82	1489.0
83	978.0
84	467.0
85	379.5
86	292.0
87	177.0
88	62.0
89	48.5
90	35.0
91	21.0
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4316078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.262800915297133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95468911724262	11.546406140390031
2	7.23290054191343	2.06322840938912
3	2.388307299510764	1.0219185461241886
4	1.2469940590461732	0.7114251202693539
5	0.8109717672946816	0.5783364432425359
6	0.5945330171728508	0.5087823636904382
7	0.41721093332553116	0.4165417537195151
8	0.34335988589854355	0.39178189558960524
9	0.2616510910290155	0.33586896785554227
>10	2.561906761976734	8.504051597481745
>50	1.080992583273002	11.292067052123848
>100	2.079824913042128	59.222417084854186
>500	0.023714197820850196	2.140161739856503
>1k	0.0026167390698869186	0.6277798439738642
>5k	1.635461918679324E-4	0.1536501256736835
>10k+	1.635461918679324E-4	0.48558291576576124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20817	0.4823128775707946	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6587	0.15261540685780006	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4715	0.10924269672605547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3169182762684084E-5	0.0	0.0	0.015013630430219287	0.0
2	2.3169182762684084E-5	0.0	0.0	0.05523533170623886	0.0
3	2.3169182762684084E-5	0.0	0.0	0.08213475289371508	0.0
4	2.3169182762684084E-5	0.0	0.0	0.12729149009818636	0.0
5	4.633836552536817E-5	0.0	0.0	0.21322598896498163	0.0
6	4.633836552536817E-5	0.0	0.0	0.34688900432290615	0.0
7	4.633836552536817E-5	0.0	0.0	0.4200341143046998	0.0
8	4.633836552536817E-5	0.0	0.0	0.6234595389610661	0.0
9	4.633836552536817E-5	0.0	0.0	0.7238516078717762	0.0
10	4.633836552536817E-5	0.0	0.0	0.8814715582063161	0.0
11	4.633836552536817E-5	0.0	0.0	1.005357178438388	0.0
12	4.633836552536817E-5	0.0	0.0	1.1139974764126135	0.0
13	6.950754828805226E-5	0.0	0.0	1.1606602104966592	0.0
14	6.950754828805226E-5	0.0	0.0	1.1794735868999586	0.0
15	6.950754828805226E-5	0.0	0.0	1.2057937785183679	0.0
16	6.950754828805226E-5	0.0	0.0	1.2637630737906034	0.0
17	6.950754828805226E-5	0.0	0.0	1.3268759276361548	0.0
18	6.950754828805226E-5	0.0	0.0	1.4252522776465115	0.0
19	6.950754828805226E-5	0.0	0.0	1.4654971481052936	0.0
20	6.950754828805226E-5	0.0	0.0	1.511094099782256	0.0
21	6.950754828805226E-5	0.0	0.0	1.5723302498240301	0.0
22	6.950754828805226E-5	0.0	0.0	1.635489442035107	0.0
23	9.267673105073634E-5	0.0	0.0	1.71078928601383	0.0
24	9.267673105073634E-5	0.0	0.0	1.7682025208997614	0.0
25	9.267673105073634E-5	0.0	0.0	1.8155834996494502	0.0
26	9.267673105073634E-5	0.0	0.0	1.8667410551894568	0.0
27	9.267673105073634E-5	0.0	0.0	1.914979293701365	0.0
28	9.267673105073634E-5	0.0	0.0	1.9679440454968609	0.0
29	9.267673105073634E-5	0.0	0.0	2.024268328792946	0.0
30	1.1584591381342043E-4	0.0	0.0	2.1061945590417968	0.0
31	1.3901509657610452E-4	0.0	0.0	2.175609430598798	0.0
32	1.3901509657610452E-4	0.0	0.0	2.2424756920519044	0.0
33	1.3901509657610452E-4	0.0	0.0	2.309179769225672	0.0
34	1.3901509657610452E-4	0.0	0.0	2.3773435049134886	0.0
35	1.3901509657610452E-4	0.0	0.0	2.465988798163518	0.0
36	1.3901509657610452E-4	0.0	0.0	2.541080119497377	0.0
37	1.3901509657610452E-4	0.0	0.0	2.616565316938202	0.0
38	1.3901509657610452E-4	0.0	0.0	2.693255311882686	0.0
39	1.3901509657610452E-4	0.0	0.0	2.7808579919083947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.035448E-4	45.000004	33
TAATGCG	95	0.0	42.63158	1
TAGTACG	135	0.0	41.666664	1
CGTTTTT	14920	0.0	41.335457	1
AAATGCG	285	0.0	41.05263	1
CGGTCTA	350	0.0	39.857143	31
CGTAAGG	800	0.0	39.375004	2
GCTACGA	600	0.0	39.375004	10
GCGTAAG	410	0.0	38.963413	1
CGAATAT	585	0.0	38.846157	14
TAGGGAC	3215	0.0	38.7014	5
TAGGGCG	1930	0.0	38.58808	5
CGCGAAA	35	6.252292E-6	38.571426	14
TAAGGGA	4180	0.0	38.00239	4
TCGTAAG	225	0.0	38.0	1
CGAGGGA	2345	0.0	37.899788	4
TACGGGA	1380	0.0	37.826088	4
CATACGA	1355	0.0	37.693726	18
ATAGGGC	2025	0.0	37.666668	4
GGGCGAT	9510	0.0	37.618298	7
>>END_MODULE