FastQCFastQC Report
Thu 26 May 2016
SRR1545502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545502_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1410169
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.4098090370728615No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55310.3922224924813976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG302.1658543E-645.0000042
GCTTACC650.045.00000421
TCCGTTC302.1658543E-645.00000412
TCACGAC900.045.025
CGGGTGC551.8189894E-1245.06
AGCCGTG207.033831E-445.037
AACCGCA207.033831E-445.034
GTCGCGA351.2121927E-745.017
TCGTTAA207.033831E-445.038
ACACGTC351.2121927E-745.021
CTATCGG207.033831E-445.015
ACGTTAG351.2121927E-745.01
GCGAACG207.033831E-445.01
GCGAAAA207.033831E-445.022
CGTTGTA207.033831E-445.020
GGCGTAA351.2121927E-745.036
TTCGTTA207.033831E-445.037
TGCGCTA351.2121927E-745.012
CTACGTC207.033831E-445.034
CGGATGT207.033831E-445.020