##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545502_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1410169
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180073452189063	31.0	31.0	33.0	30.0	34.0
2	31.44832215145844	31.0	31.0	34.0	30.0	34.0
3	31.53584144879089	31.0	31.0	34.0	30.0	34.0
4	35.28695213126937	37.0	35.0	37.0	33.0	37.0
5	35.16257413118569	37.0	35.0	37.0	32.0	37.0
6	35.2002362837362	37.0	35.0	37.0	32.0	37.0
7	35.81827142704172	37.0	35.0	37.0	35.0	37.0
8	35.84288337071656	37.0	35.0	37.0	35.0	37.0
9	37.542315850086055	39.0	37.0	39.0	35.0	39.0
10	36.80636008875532	39.0	37.0	39.0	32.0	39.0
11	36.35835775711989	38.0	35.0	39.0	32.0	39.0
12	34.96345402572316	35.0	34.0	39.0	30.0	39.0
13	34.26705167962138	35.0	33.0	39.0	25.0	39.0
14	35.104428618130164	36.0	33.0	40.0	25.0	41.0
15	35.680185140929915	36.0	33.0	40.0	30.0	41.0
16	35.98349630434367	36.0	34.0	40.0	31.0	41.0
17	35.97981518527212	36.0	34.0	40.0	31.0	41.0
18	35.913437325597144	36.0	35.0	40.0	31.0	41.0
19	35.811928215696135	36.0	34.0	40.0	31.0	41.0
20	35.636498178587104	35.0	34.0	40.0	30.0	41.0
21	35.43621721935456	35.0	34.0	40.0	30.0	41.0
22	35.33291187084669	35.0	34.0	40.0	30.0	41.0
23	35.35104019447314	35.0	34.0	40.0	30.0	41.0
24	35.269428699680674	35.0	34.0	40.0	30.0	41.0
25	35.18289509980718	35.0	34.0	40.0	30.0	41.0
26	35.01392386302635	35.0	34.0	39.0	29.0	41.0
27	34.93497942445196	35.0	34.0	39.0	29.0	41.0
28	34.99805980701604	35.0	34.0	39.0	29.0	41.0
29	35.06029419168908	35.0	34.0	39.0	29.0	41.0
30	35.02368439527461	36.0	34.0	39.0	29.0	41.0
31	34.785344877103384	35.0	34.0	39.0	29.0	41.0
32	34.55540860705348	35.0	34.0	39.0	28.0	41.0
33	34.32566096687702	35.0	33.0	39.0	27.0	41.0
34	34.2097840755257	35.0	33.0	39.0	26.0	41.0
35	34.01269422317467	35.0	33.0	39.0	25.0	41.0
36	33.75115181230051	35.0	33.0	39.0	23.0	41.0
37	33.660842778418754	35.0	33.0	39.0	23.0	41.0
38	33.70435032964134	35.0	33.0	39.0	23.0	41.0
39	33.706227409622535	35.0	33.0	39.0	23.0	41.0
40	33.493927323604474	35.0	33.0	39.0	23.0	41.0
41	33.52673048407673	35.0	33.0	39.0	23.0	41.0
42	33.524503800608294	35.0	33.0	39.0	23.0	41.0
43	33.408017053275174	35.0	33.0	39.0	23.0	41.0
44	33.322943562083694	35.0	33.0	39.0	23.0	41.0
45	33.25480208400553	35.0	32.0	38.0	23.0	41.0
46	33.23604121208167	35.0	32.0	38.0	23.0	40.0
47	33.16445830251551	35.0	32.0	38.0	23.0	40.0
48	33.17506483265481	35.0	32.0	38.0	23.0	40.0
49	33.20445492703357	35.0	32.0	38.0	24.0	40.0
50	33.07173537356161	35.0	32.0	38.0	23.0	40.0
51	32.888816872303956	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	1.0
13	5.0
14	12.0
15	51.0
16	163.0
17	410.0
18	929.0
19	1824.0
20	3143.0
21	4953.0
22	7537.0
23	10882.0
24	15660.0
25	21320.0
26	27571.0
27	32287.0
28	35128.0
29	40039.0
30	47508.0
31	58555.0
32	74017.0
33	96556.0
34	169896.0
35	255875.0
36	85504.0
37	97664.0
38	130155.0
39	192373.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.6520707801689	25.530415148822588	29.358041482971192	14.45947258803732
2	29.196571474766497	26.67198045057011	31.267458013897624	12.863990060765767
3	27.01342888689228	25.57955819479793	33.347917873673296	14.059095044636493
4	23.37492882058817	29.471644887953147	32.40157739958828	14.751848891870406
5	22.682742281244305	32.22415185697601	30.537970980783154	14.555134880996533
6	19.99710672976076	40.288433513997255	29.22408590743379	10.490373848808192
7	87.14870345327405	4.920048589920783	6.56460324968142	1.3666447071237562
8	89.26809481700421	3.1957162581222534	5.880075366853193	1.6561135580203508
9	85.18695276949076	4.721845395835535	7.8245940734763	2.2666077611974167
10	59.32494615893557	20.627243968630708	11.139303161535958	8.908506710897772
11	56.32438381498955	17.3563594150772	17.051147770231793	9.268108999701456
12	49.617244457933765	20.374791957559697	19.525106565241472	10.482857019265067
13	20.422445820323663	49.55420236865227	19.806278538246126	10.217073272777943
14	12.724645060272918	50.59684335707281	25.253923465910823	11.424588116743454
15	10.588163546355082	21.554437801426637	55.77693170109398	12.080466951124299
16	11.940696469713915	15.9327002650037	54.671745017795736	17.454858247486648
17	12.978869908500329	15.55657513390239	28.561399378372382	42.9031555792249
18	19.22726992296668	21.357511050093997	37.918859370756266	21.496359656183053
19	30.442734168741474	22.843361327613923	25.24867586792789	21.465228635716713
20	33.004838427167236	21.803131397726087	24.29673322842865	20.895296946678023
21	19.632044102515373	31.536078299834987	28.108191287710905	20.72368630993874
22	21.97495477492414	24.950130090790537	23.757719819397533	29.31719531488779
23	17.07717301968771	32.691046250484874	23.91266578686668	26.319114942960738
24	17.53846524778236	23.009298885452736	44.45325347529268	14.998982391472227
25	13.8423125171522	23.767009486097056	41.92809514320624	20.462582853544504
26	13.952015680390081	39.14729369316727	27.473657412693086	19.42703321374956
27	15.671242241178184	39.37584785937005	27.79496641891858	17.157943480533184
28	12.222223010149849	30.344518990277052	42.36626957478146	15.066988424791639
29	12.66387220255161	23.15743715824132	42.00985839285929	22.168832246347776
30	16.464125931005434	34.22781241113654	31.680032676934466	17.628028980923563
31	30.68532920522292	26.921383181732118	24.02236895010456	18.3709186629404
32	32.681614756812834	25.424895881273805	26.702898730577683	15.190590631335677
33	30.4316007513993	26.64843717313315	23.782823193532124	19.137138881935428
34	18.72449330541233	27.327930198437205	27.479188664621052	26.468387831529412
35	18.874829896274843	24.86581395563227	33.17049233106103	23.08886381703186
36	34.67832578932028	22.550204975432024	24.81943653562091	17.952032699626784
37	19.305558411793196	34.531605786256826	28.752865791263314	17.409970010686663
38	20.08035916262519	35.96767479642511	22.102031742294717	21.849934298654983
39	19.598289283057564	34.16016094524841	26.343083701315233	19.8984660703788
40	25.4609199322918	25.327957145561985	23.958617725960504	25.252505196185705
41	15.614653279145973	23.334508133422304	27.265809984477034	33.78502860295468
42	20.873313765938693	24.26453850566847	24.322616650912053	30.539531077480785
43	20.91316714521451	26.378469530956927	25.81704746026895	26.89131586355962
44	18.499697553981118	30.81928478076032	29.47639609153229	21.20462157372627
45	14.819216703813515	42.36917702771795	21.119738130677952	21.691868137790575
46	21.13363717398411	36.20332031125347	24.52550013509019	18.137542379672222
47	21.300709347603018	27.402602099464673	25.657350289220656	25.639338263711654
48	22.497090774226354	23.388189642518025	32.70359793755217	21.411121645703457
49	20.911819788975645	22.521626840470894	34.66655415060181	21.899999219951653
50	19.123098011656758	36.765664257262785	24.795255036807646	19.315982694272815
51	17.173544447509485	37.831777609634024	21.976656698594283	23.01802124426221
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1341.0
1	1565.0
2	1789.0
3	5437.5
4	9086.0
5	6820.5
6	4555.0
7	5212.5
8	5870.0
9	6828.5
10	7787.0
11	8158.5
12	8530.0
13	8431.5
14	8333.0
15	7786.5
16	7240.0
17	6954.5
18	6669.0
19	6354.5
20	6040.0
21	6134.5
22	6229.0
23	5671.5
24	5114.0
25	5741.0
26	7813.0
27	9258.0
28	10446.0
29	11634.0
30	14508.0
31	17382.0
32	20053.0
33	22724.0
34	27207.0
35	31690.0
36	33285.0
37	34880.0
38	44860.0
39	54840.0
40	85842.5
41	116845.0
42	148688.5
43	180532.0
44	179493.5
45	178455.0
46	163358.0
47	148261.0
48	131129.5
49	113998.0
50	106074.0
51	98150.0
52	89341.0
53	80532.0
54	69279.5
55	58027.0
56	51194.5
57	44362.0
58	39799.5
59	35237.0
60	32537.5
61	29838.0
62	24763.5
63	19689.0
64	16628.0
65	13567.0
66	11241.0
67	8915.0
68	7574.5
69	6234.0
70	4804.0
71	3374.0
72	2749.0
73	2124.0
74	2134.0
75	1685.0
76	1226.0
77	908.0
78	590.0
79	535.0
80	480.0
81	301.0
82	122.0
83	105.0
84	88.0
85	50.5
86	13.0
87	10.0
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1410169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.3543825344488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53974818129271	14.150719817007795
2	7.294060521653761	2.5316783304440107
3	2.430853453704228	1.265578821623076
4	1.1435126270492029	0.793798222511374
5	0.6887593358507496	0.5976496494263402
6	0.4583444082956097	0.4772570516452559
7	0.3505332338993214	0.4258301482488368
8	0.29160401231137134	0.40484860625853236
9	0.22744420297721307	0.35524383333384374
>10	2.6827321797977315	10.994865732114603
>50	1.1734818363671689	14.815682265823154
>100	1.7095217969635281	51.35314127961503
>500	0.00735981639448541	0.7708207919775076
>1k	0.0012266360657475685	0.2603462658349881
>5k	8.177573771650458E-4	0.8025391841356542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5779	0.4098090370728615	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5531	0.3922224924813976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03652044542178987	0.0
2	0.0	0.0	0.0	0.12828249663692792	0.0
3	0.0	0.0	0.0	0.18735343068809482	0.0
4	0.0	0.0	0.0	0.3064880875980113	0.0
5	0.0	0.0	0.0	0.5149028237041092	0.0
6	0.0	0.0	0.0	0.7716805574367328	0.0
7	0.0	0.0	0.0	0.9027995935238968	0.0
8	0.0	0.0	0.0	1.3013333862820697	0.0
9	0.0	0.0	0.0	1.475922389444102	0.0
10	0.0	0.0	0.0	1.770213357406098	0.0
11	0.0	0.0	0.0	2.001887717004132	0.0
12	0.0	0.0	0.0	2.2190248119197062	0.0
13	7.091348625590267E-5	0.0	0.0	2.3002916671689704	0.0
14	7.091348625590267E-5	0.0	0.0	2.3293661965338908	0.0
15	7.091348625590267E-5	0.0	0.0	2.373474384985062	0.0
16	7.091348625590267E-5	0.0	0.0	2.473320573633373	0.0
17	7.091348625590267E-5	0.0	0.0	2.5990501847650886	0.0
18	7.091348625590267E-5	0.0	0.0	2.8066139590361154	0.0
19	7.091348625590267E-5	0.0	0.0	2.8795839363934395	7.091348625590267E-5
20	7.091348625590267E-5	0.0	0.0	2.96056713769768	7.091348625590267E-5
21	7.091348625590267E-5	0.0	0.0	3.0694902525867467	7.091348625590267E-5
22	7.091348625590267E-5	0.0	0.0	3.17656961683316	7.091348625590267E-5
23	7.091348625590267E-5	0.0	0.0	3.3028665358549225	7.091348625590267E-5
24	7.091348625590267E-5	0.0	0.0	3.3976778669790644	7.091348625590267E-5
25	7.091348625590267E-5	0.0	0.0	3.4654002463534512	7.091348625590267E-5
26	7.091348625590267E-5	0.0	0.0	3.543688735179968	7.091348625590267E-5
27	7.091348625590267E-5	0.0	0.0	3.619495251987528	7.091348625590267E-5
28	7.091348625590267E-5	0.0	0.0	3.694308979987505	7.091348625590267E-5
29	7.091348625590267E-5	0.0	0.0	3.7764977105580964	7.091348625590267E-5
30	7.091348625590267E-5	0.0	0.0	3.903361937469906	7.091348625590267E-5
31	7.091348625590267E-5	0.0	0.0	4.016185294103047	7.091348625590267E-5
32	7.091348625590267E-5	0.0	0.0	4.106245421648044	7.091348625590267E-5
33	1.4182697251180533E-4	0.0	0.0	4.200702185340906	7.091348625590267E-5
34	1.4182697251180533E-4	0.0	0.0	4.302463038118126	7.091348625590267E-5
35	1.4182697251180533E-4	0.0	0.0	4.455777995403388	7.091348625590267E-5
36	1.4182697251180533E-4	0.0	0.0	4.566544860935108	7.091348625590267E-5
37	1.4182697251180533E-4	0.0	0.0	4.6801482659170635	7.091348625590267E-5
38	1.4182697251180533E-4	0.0	0.0	4.776306953280068	7.091348625590267E-5
39	1.4182697251180533E-4	0.0	0.0	4.883315404040225	7.091348625590267E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	30	2.1658543E-6	45.000004	2
GCTTACC	65	0.0	45.000004	21
TCCGTTC	30	2.1658543E-6	45.000004	12
TCACGAC	90	0.0	45.0	25
CGGGTGC	55	1.8189894E-12	45.0	6
AGCCGTG	20	7.033831E-4	45.0	37
AACCGCA	20	7.033831E-4	45.0	34
GTCGCGA	35	1.2121927E-7	45.0	17
TCGTTAA	20	7.033831E-4	45.0	38
ACACGTC	35	1.2121927E-7	45.0	21
CTATCGG	20	7.033831E-4	45.0	15
ACGTTAG	35	1.2121927E-7	45.0	1
GCGAACG	20	7.033831E-4	45.0	1
GCGAAAA	20	7.033831E-4	45.0	22
CGTTGTA	20	7.033831E-4	45.0	20
GGCGTAA	35	1.2121927E-7	45.0	36
TTCGTTA	20	7.033831E-4	45.0	37
TGCGCTA	35	1.2121927E-7	45.0	12
CTACGTC	20	7.033831E-4	45.0	34
CGGATGT	20	7.033831E-4	45.0	20
>>END_MODULE