##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545501_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1753560
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0865456556947	31.0	31.0	33.0	30.0	34.0
2	31.368468715071057	31.0	31.0	34.0	30.0	34.0
3	31.467276853942835	31.0	31.0	34.0	30.0	34.0
4	35.239210520312966	37.0	35.0	37.0	33.0	37.0
5	35.10191496156391	37.0	35.0	37.0	32.0	37.0
6	35.15558692032209	37.0	35.0	37.0	32.0	37.0
7	35.77128470083716	37.0	35.0	37.0	35.0	37.0
8	35.766184789799034	37.0	35.0	37.0	35.0	37.0
9	37.4318437920573	39.0	37.0	39.0	35.0	39.0
10	36.65018533725678	39.0	35.0	39.0	32.0	39.0
11	36.271203722712656	38.0	35.0	39.0	31.0	39.0
12	35.48420983599078	37.0	35.0	39.0	30.0	39.0
13	35.12025080407856	37.0	34.0	39.0	30.0	39.0
14	36.06030646228244	38.0	35.0	40.0	30.0	41.0
15	36.36730479709848	38.0	35.0	40.0	30.0	41.0
16	36.53689922215379	38.0	35.0	40.0	31.0	41.0
17	36.479783412030386	38.0	35.0	40.0	31.0	41.0
18	36.40117703414768	38.0	35.0	40.0	31.0	41.0
19	36.31719929742923	37.0	35.0	40.0	31.0	41.0
20	36.18138301512352	37.0	34.0	40.0	31.0	41.0
21	36.04130967859668	37.0	34.0	40.0	30.0	41.0
22	35.91545712721549	37.0	34.0	40.0	30.0	41.0
23	35.877314149501586	37.0	34.0	40.0	30.0	41.0
24	35.79977987636579	37.0	34.0	40.0	30.0	41.0
25	35.67578982184813	36.0	34.0	40.0	30.0	41.0
26	35.52968190424052	36.0	34.0	40.0	29.0	41.0
27	35.437776865348205	36.0	34.0	40.0	29.0	41.0
28	35.44193355231643	36.0	34.0	40.0	29.0	41.0
29	35.458542051597895	36.0	34.0	40.0	29.0	41.0
30	35.394322977257694	36.0	34.0	40.0	29.0	41.0
31	35.225260042427976	36.0	34.0	40.0	29.0	41.0
32	35.06207258377244	36.0	34.0	40.0	27.0	41.0
33	34.842957184242344	36.0	33.0	40.0	27.0	41.0
34	34.686898081616825	36.0	34.0	40.0	26.0	41.0
35	34.53425716827483	36.0	33.0	40.0	25.0	41.0
36	34.33954184630124	36.0	33.0	40.0	24.0	41.0
37	34.2564354798239	36.0	33.0	40.0	24.0	41.0
38	34.25599637309245	35.0	33.0	40.0	24.0	41.0
39	34.19297942471316	36.0	33.0	40.0	24.0	41.0
40	34.057008029380235	35.0	33.0	40.0	23.0	41.0
41	34.03582939848081	35.0	33.0	40.0	23.0	41.0
42	33.99854695590684	35.0	33.0	40.0	23.0	41.0
43	33.94640559775542	35.0	33.0	40.0	23.0	41.0
44	33.82803496886334	35.0	33.0	40.0	23.0	41.0
45	33.74247017495837	35.0	33.0	40.0	23.0	41.0
46	33.69281632792719	35.0	33.0	39.0	23.0	41.0
47	33.61754545039805	35.0	33.0	39.0	23.0	41.0
48	33.57052282214467	35.0	32.0	39.0	23.0	41.0
49	33.5503792285408	35.0	32.0	39.0	23.0	41.0
50	33.4792547731472	35.0	32.0	39.0	23.0	41.0
51	33.31989495654554	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	1.0
13	2.0
14	14.0
15	63.0
16	178.0
17	485.0
18	1172.0
19	2259.0
20	3988.0
21	6013.0
22	8993.0
23	12989.0
24	18016.0
25	24716.0
26	31795.0
27	37011.0
28	41566.0
29	47997.0
30	56781.0
31	69190.0
32	85142.0
33	107917.0
34	171906.0
35	237158.0
36	132231.0
37	157380.0
38	206868.0
39	291550.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.44568762973608	24.337861265083603	28.84030201418828	15.376149090992039
2	30.983599078446133	26.19653732977486	28.983211295878096	13.836652295900912
3	27.75861675676909	25.596329752047264	32.07053080590342	14.57452268528023
4	25.45102534273136	28.590011177262255	30.73781336253108	15.221150117475307
5	22.322589475124886	33.0046305800771	30.430951892150826	14.241828052647188
6	20.962499144597277	41.24244394260818	27.015670977896395	10.77938593489815
7	87.48477383151987	4.116825201304774	6.804443532014873	1.5939574351604737
8	88.4559980838979	3.2603389675859393	6.497753142179338	1.7859098063368235
9	83.28417619015033	5.543066675790962	8.23804146992404	2.9347156641346746
10	48.61647163484569	27.635096603480918	13.402278792855677	10.34615296881772
11	41.63974999429732	21.945071739775084	22.245831337393646	14.169346928533955
12	38.58134309632975	21.172186865576315	25.645600948926756	14.60086908916718
13	21.606389288076826	37.52623235019047	25.66031387577271	15.20706448595999
14	16.679953922306623	39.58461643741874	27.882593124843176	15.852836515431465
15	14.852528570450968	23.13527908939529	45.68540568899838	16.326786651155363
16	17.322019206642487	19.7738885467278	43.63534752161318	19.268744725016536
17	17.726852802299323	19.48789890280344	29.356337963913408	33.42891033098383
18	20.961985902963114	23.138757727137936	34.78056068797189	21.11869568192705
19	28.19270512557312	23.755560117703414	26.59355824722279	21.458176509500674
20	30.250005702684824	23.728643445334065	25.948641620474923	20.072709231506195
21	23.25657519560209	28.399085289354225	27.87181505052579	20.472524464517893
22	23.558418303337213	24.418212094253974	25.08633864823559	26.937030954173224
23	20.158249503866422	29.978500878213467	24.99538082529255	24.867868792627572
24	19.914630808184494	23.469969661716735	38.28189511622072	18.333504413878053
25	18.387908027099158	26.16830903989598	34.94337233969753	20.50041059330733
26	18.301455325167087	32.39951869340085	27.886413923675267	21.412612057756792
27	18.527053536805127	33.41180227651178	27.855904559866783	20.205239626816304
28	16.977748123816696	28.359394602979084	35.89937042359543	18.763486849608796
29	17.369864732315975	24.06966399781017	35.424507858299684	23.13596341157417
30	18.967928100549738	30.60243162480896	30.885969114258994	19.54367116038231
31	27.422785647482833	27.866853714728894	25.279032368439065	19.43132826934921
32	29.576062410182715	25.815826090923604	26.241645566732817	18.36646593216086
33	28.33179360843085	27.50804078560186	24.96954766303976	19.190617942927528
34	21.527236022719496	26.7701133693743	27.45894066926709	24.24370993863911
35	21.306085905244192	26.251967426264283	29.854068295353454	22.587878373138075
36	29.96133579689318	26.08527794885832	24.933677775496704	19.019708478751795
37	22.13474303702183	32.57578868131116	27.675927826820867	17.61354045484614
38	22.551552270809097	32.023825817194734	24.20219439312028	21.222427518875886
39	21.35461575309656	32.07674673236159	26.142304797098475	20.426332717443373
40	25.11428180387326	26.20902620953945	25.050012545906615	23.62667944068067
41	18.575070143023336	25.77151622984101	27.3714614840667	28.281952143068956
42	21.398469399393235	26.650699149159422	24.828007025707706	27.12282442573964
43	21.70139601724492	26.429320924291154	26.575765870571864	25.29351718789206
44	20.906384725928966	29.02210360637788	28.23695795980748	21.834553707885675
45	18.510914938753164	35.78588699559753	24.00893040443441	21.6942676612149
46	21.65640183398344	32.75747621980428	25.911745249663543	19.674376696548734
47	21.521875498984922	28.041412897191996	26.603823079906018	23.83288852391706
48	22.485857341636443	25.112000729943656	30.01568238326604	22.38645954515386
49	21.102614110723326	24.608966901617283	32.11022149227856	22.178197495380825
50	20.376605305777957	32.810054973881705	26.598975797805608	20.21436392253473
51	19.230764844088597	32.52623235019047	24.765049385250578	23.477953420470357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1516.0
1	1938.0
2	2360.0
3	8952.0
4	15544.0
5	11285.5
6	7027.0
7	7397.0
8	7767.0
9	8287.0
10	8807.0
11	8881.0
12	8955.0
13	8881.0
14	8807.0
15	8604.0
16	8401.0
17	8176.0
18	7951.0
19	7790.5
20	7630.0
21	7331.5
22	7033.0
23	7283.0
24	7533.0
25	8284.0
26	10407.5
27	11780.0
28	16016.0
29	20252.0
30	23577.0
31	26902.0
32	30635.0
33	34368.0
34	41280.5
35	48193.0
36	51742.0
37	55291.0
38	62850.5
39	70410.0
40	94182.0
41	117954.0
42	137724.0
43	157494.0
44	161991.0
45	166488.0
46	159271.0
47	152054.0
48	146662.5
49	141271.0
50	134355.5
51	127440.0
52	117052.5
53	106665.0
54	96144.5
55	85624.0
56	79534.0
57	73444.0
58	69459.0
59	65474.0
60	58712.5
61	51951.0
62	45704.5
63	39458.0
64	34225.5
65	28993.0
66	25105.5
67	21218.0
68	18705.0
69	16192.0
70	12874.5
71	9557.0
72	8312.5
73	7068.0
74	5625.5
75	3394.5
76	2606.0
77	2034.0
78	1462.0
79	1125.0
80	788.0
81	555.0
82	322.0
83	197.5
84	73.0
85	121.0
86	169.0
87	100.0
88	31.0
89	21.5
90	12.0
91	8.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1753560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.288199956814907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20203013190951	15.276645941699499
2	6.9366838776439215	2.6759229133842033
3	2.6093260482632776	1.509876077142833
4	1.4246524130134282	1.0991592244464743
5	0.9450928181409891	0.9114569627026552
6	0.688636925714375	0.7969540032495094
7	0.5096503815346809	0.6881166926975553
8	0.4416422652983172	0.681478745796371
9	0.3588773730577176	0.6229888678362344
>10	4.144439464387986	18.843068923931444
>50	1.5227689401028024	21.427912621512192
>100	1.2141259572489806	34.47215518413397
>500	8.886015788494533E-4	0.09426738714134326
>1k	8.886015788494533E-4	0.36787133685036927
>5k	2.962005262831511E-4	0.5321251174754371
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9314	0.5311480645087707	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3706	0.21134149957800133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014998061087159835	0.0
2	0.0	0.0	0.0	0.06911654006706357	0.0
3	0.0	0.0	0.0	0.10971965601405141	0.0
4	0.0	0.0	0.0	0.17433107507014303	0.0
5	0.0	0.0	0.0	0.3035539131823262	0.0
6	0.0	0.0	0.0	0.5054859828007026	0.0
7	0.0	0.0	0.0	0.6168023905654783	0.0
8	0.0	0.0	0.0	0.9155090216473916	0.0
9	0.0	0.0	0.0	1.0672004379661946	0.0
10	0.0	0.0	0.0	1.3074545496019525	0.0
11	0.0	0.0	0.0	1.486803987317229	0.0
12	5.7026848240151464E-5	0.0	0.0	1.6502999612217433	0.0
13	5.7026848240151464E-5	0.0	0.0	1.723978649148019	0.0
14	5.7026848240151464E-5	0.0	0.0	1.7557996304660235	0.0
15	5.7026848240151464E-5	0.0	0.0	1.7946919409658066	0.0
16	5.7026848240151464E-5	0.0	0.0	1.8752708775291407	0.0
17	5.7026848240151464E-5	0.0	0.0	1.9704486872419535	0.0
18	5.7026848240151464E-5	0.0	0.0	2.1205433518100323	0.0
19	5.7026848240151464E-5	0.0	0.0	2.1836150459636396	0.0
20	5.7026848240151464E-5	0.0	0.0	2.2607723716325645	0.0
21	5.7026848240151464E-5	0.0	0.0	2.349049932708319	0.0
22	5.7026848240151464E-5	0.0	0.0	2.447706380163781	0.0
23	5.7026848240151464E-5	0.0	0.0	2.562900613608887	0.0
24	5.7026848240151464E-5	0.0	0.0	2.648041698031433	0.0
25	5.7026848240151464E-5	0.0	0.0	2.7162458085266543	0.0
26	5.7026848240151464E-5	0.0	0.0	2.787757476219804	0.0
27	5.7026848240151464E-5	0.0	0.0	2.860295627181277	0.0
28	5.7026848240151464E-5	0.0	0.0	2.935228905768836	0.0
29	5.7026848240151464E-5	0.0	0.0	3.024475923264673	0.0
30	5.7026848240151464E-5	0.0	0.0	3.15501037888638	0.0
31	5.7026848240151464E-5	0.0	0.0	3.2547503364584047	0.0
32	5.7026848240151464E-5	0.0	0.0	3.3470197769109697	0.0
33	5.7026848240151464E-5	0.0	0.0	3.4431670430438652	0.0
34	1.1405369648030293E-4	0.0	0.0	3.5494650881635073	0.0
35	1.1405369648030293E-4	0.0	0.0	3.6793722484545723	0.0
36	1.1405369648030293E-4	0.0	0.0	3.786468669449577	0.0
37	1.1405369648030293E-4	0.0	0.0	3.895275895891786	0.0
38	1.1405369648030293E-4	0.0	0.0	4.003569880699834	0.0
39	1.1405369648030293E-4	0.0	0.0	4.120189785350943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.034301E-4	45.000004	34
ATTACGC	20	7.034301E-4	45.000004	11
GTCGTAC	30	2.166158E-6	45.000004	19
AGTACGC	20	7.034301E-4	45.000004	21
TCGCGAC	20	7.034301E-4	45.000004	29
ACGCGCC	20	7.034301E-4	45.000004	35
AACCGTA	25	3.891648E-5	45.0	12
ATCGTAG	35	1.212411E-7	45.0	1
TACAACG	45	3.8562575E-10	45.0	1
TACCGGA	25	3.891648E-5	45.0	14
AATCGTG	100	0.0	42.75	1
CGCATCG	85	0.0	42.35294	21
CGTTTTT	6905	0.0	41.80666	1
TATTACG	55	6.184564E-11	40.909092	1
TAACGGG	525	0.0	40.714283	3
TATAGCG	155	0.0	40.64516	1
CTAACGG	50	1.0822987E-9	40.5	2
GGACGTA	50	1.0822987E-9	40.5	8
CGACGGT	295	0.0	40.42373	28
CACGACG	295	0.0	40.42373	26
>>END_MODULE