FastQCFastQC Report
Thu 26 May 2016
SRR1545500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545500_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2578550
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110650.4291171394776134No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC75610.2932268135192259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA253.8921607E-545.022
GTCGACC207.034921E-445.011
TAGAACG1100.045.01
CCGTACA253.8921607E-545.043
CTACGTC406.8212103E-945.043
CGTACAC207.034921E-445.015
TCGTAGC406.8212103E-945.018
TCGACCA351.2127202E-745.038
TATCGTG502.1827873E-1145.01
TAGCTAC207.034921E-445.027
CAATACG253.8921607E-545.01
AGTCGAT700.045.010
GTACGAT253.8921607E-545.013
AAATGCG750.045.01
AAGTCGA453.8562575E-1045.017
TCTAGCG1000.042.751
TCACGAC1900.042.6315825
TTCTACG750.042.01
TAGTCCG650.041.538461
ACGTTAG603.6379788E-1241.2499961
Produced by FastQC (version 0.11.5)