##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545500_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2578550
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09952958057823	31.0	31.0	33.0	30.0	34.0
2	31.37852320102383	31.0	31.0	34.0	30.0	34.0
3	31.484262085280488	31.0	31.0	34.0	30.0	34.0
4	35.25044191502977	37.0	35.0	37.0	33.0	37.0
5	35.1193713521165	37.0	35.0	37.0	32.0	37.0
6	35.169178026410194	37.0	35.0	37.0	32.0	37.0
7	35.759381435302785	37.0	35.0	37.0	35.0	37.0
8	35.756283182408716	37.0	35.0	37.0	35.0	37.0
9	37.42851176048554	39.0	37.0	39.0	35.0	39.0
10	36.653119776618645	39.0	35.0	39.0	32.0	39.0
11	36.29437435768164	38.0	35.0	39.0	32.0	39.0
12	35.302684842256305	37.0	35.0	39.0	30.0	39.0
13	34.84911442477361	37.0	33.0	39.0	27.0	39.0
14	35.76415272149076	38.0	34.0	40.0	27.0	41.0
15	36.13917395435419	38.0	34.0	40.0	30.0	41.0
16	36.32709080684881	38.0	35.0	40.0	31.0	41.0
17	36.26542242733319	37.0	35.0	40.0	31.0	41.0
18	36.20881541951872	37.0	35.0	40.0	31.0	41.0
19	36.13507669038801	37.0	35.0	40.0	30.0	41.0
20	36.00428574198678	37.0	34.0	40.0	30.0	41.0
21	35.836533322991606	36.0	34.0	40.0	30.0	41.0
22	35.73057377208121	36.0	34.0	40.0	30.0	41.0
23	35.69666168970933	36.0	34.0	40.0	30.0	41.0
24	35.602344728626555	36.0	34.0	40.0	30.0	41.0
25	35.49395823233988	36.0	34.0	40.0	29.0	41.0
26	35.316909503403075	36.0	34.0	40.0	29.0	41.0
27	35.21766186422602	36.0	34.0	40.0	29.0	41.0
28	35.2443815322565	36.0	34.0	40.0	29.0	41.0
29	35.26277442748832	36.0	34.0	40.0	29.0	41.0
30	35.20599018828411	36.0	34.0	40.0	29.0	41.0
31	35.03570572608637	36.0	34.0	40.0	29.0	41.0
32	34.87758895503287	35.0	34.0	40.0	27.0	41.0
33	34.67885206802273	35.0	33.0	40.0	27.0	41.0
34	34.547438676775705	35.0	33.0	40.0	26.0	41.0
35	34.38953132574509	35.0	33.0	40.0	25.0	41.0
36	34.22441953811251	35.0	33.0	40.0	24.0	41.0
37	34.122252040875686	35.0	33.0	40.0	24.0	41.0
38	34.107257955052255	35.0	33.0	40.0	24.0	41.0
39	34.04408020011247	35.0	33.0	40.0	24.0	41.0
40	33.87364836826899	35.0	33.0	40.0	23.0	41.0
41	33.86552713734463	35.0	33.0	39.0	23.0	41.0
42	33.839151848907335	35.0	33.0	39.0	23.0	41.0
43	33.751422698803594	35.0	33.0	39.0	23.0	41.0
44	33.642921021504335	35.0	33.0	39.0	23.0	41.0
45	33.55999883655543	35.0	32.0	39.0	23.0	41.0
46	33.512531461480286	35.0	32.0	39.0	23.0	41.0
47	33.43943805627194	35.0	32.0	39.0	23.0	41.0
48	33.400800837680094	35.0	32.0	39.0	23.0	41.0
49	33.389009714762174	35.0	32.0	39.0	23.0	41.0
50	33.29854104050726	35.0	32.0	39.0	23.0	40.0
51	33.126685152508195	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	6.0
14	26.0
15	93.0
16	265.0
17	794.0
18	1835.0
19	3410.0
20	5822.0
21	9026.0
22	13571.0
23	19461.0
24	26755.0
25	36320.0
26	46819.0
27	55425.0
28	63651.0
29	73730.0
30	87214.0
31	105295.0
32	129360.0
33	165627.0
34	272371.0
35	376231.0
36	186468.0
37	215982.0
38	286444.0
39	396277.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.978088460568927	24.764848461344556	28.80254406546315	14.454519012623374
2	30.53902387000446	26.425588024277207	29.79829749277695	13.237090612941383
3	29.330864245409245	26.224583583797095	31.147195129045397	13.29735704174827
4	25.32667584495162	28.456458086909308	30.794516297919373	15.422349770219698
5	22.60382773264044	32.83969672878168	30.033468422175254	14.523007116402628
6	21.46880999011072	41.118070233270636	26.96403017199589	10.449089604622754
7	87.71600318008183	4.51269124120145	6.187586046421439	1.5837195322952822
8	88.87204048787109	3.4780012022260576	5.897616877702585	1.7523414322002675
9	83.8674061003277	5.240038005856004	7.920265265362316	2.972290628453976
10	51.803959589691885	23.74190921254193	13.029415756917647	11.424715440848539
11	47.87105155998526	20.316456923464738	18.699501657908517	13.112989858641486
12	42.47371584805414	20.449399856508503	22.704000310251885	14.372883985185473
13	22.135541292586918	39.576893990808784	23.231040701169263	15.05652401543503
14	17.048806499777005	41.683775765449575	26.448973260165598	14.818444474607823
15	15.183882414535303	22.12309243567121	47.38213336952939	15.310891780264102
16	17.29972271237711	17.585503480638344	45.74997576157143	19.36479804541312
17	17.61978631401369	17.60396346784045	28.734172306141048	36.042077912004814
18	21.66605262647612	22.10672664869791	34.90601306936069	21.32120765546528
19	30.70908068488104	22.91260592193287	25.185045859106864	21.19326753407923
20	32.075740241608656	22.80192356169165	24.54538403366233	20.576952163037365
21	23.75412538054333	27.121483004013886	27.815322565007467	21.309069050435323
22	23.91681371313335	24.22027883888232	23.630994163386397	28.23191328459793
23	20.452347249423124	29.717244187624832	24.587539508638574	25.24286905431347
24	21.050163851777164	22.550348063834324	38.366019662213255	18.033468422175254
25	19.118574392584982	23.665238215276027	35.166779779333346	22.049407612805645
26	17.75265168408602	32.98632952628415	27.115355529270325	22.145663260359505
27	19.2257276376258	33.30076981249152	27.476876539140214	19.99662601074247
28	16.368656803242132	28.721801012196778	36.84419538112505	18.06534680343604
29	17.57433441275135	24.58556165286692	34.76957980260224	23.07052413177949
30	19.62032925481375	30.182428108820847	30.255220957514883	19.942021678850516
31	29.407186209303678	26.609451048069648	24.41798685307634	19.565375889550328
32	32.37032440712804	24.636287836187005	26.011750790172773	16.981636966512188
33	29.488472203370108	25.590622636753213	23.928835973706153	20.99206918617052
34	20.730410502026334	26.793081382947783	27.439297279478776	25.037210835547107
35	21.474976246339995	25.271179538888134	30.04514164937659	23.20870256539528
36	31.867677570727736	24.86959725427081	23.98522425394117	19.277500921060287
37	21.702584786023152	33.0966628531539	26.919004867076456	18.281747493746487
38	22.795408272090903	32.41310038587578	22.343216148610654	22.44827519342266
39	22.533710806460995	30.932306916677977	25.45341374028039	21.08056853658064
40	24.825076108665723	24.938162921021505	25.266021601287548	24.970739369025228
41	17.94915747222276	24.37955440072909	27.355606833297784	30.315681293750362
42	21.936359581935584	25.620057784413717	24.080549145837775	28.363033487812917
43	22.352562486668866	24.822865563979757	26.423842857419867	26.400729091931513
44	20.7827655077466	28.37699482267166	28.326229857865854	22.514009811715887
45	18.69725233173683	35.878071008900356	23.362858971127185	22.061817688235635
46	22.340152411238876	31.767311085687695	25.17500145430571	20.71753504876772
47	22.41984836439084	26.22268329099688	26.682670493106592	24.67479785150569
48	23.331639875123617	24.233697232940994	30.291210176261853	22.143452715673536
49	22.50908456302961	24.50237536600027	30.552713734463165	22.43582633650695
50	20.74949099299994	32.800760117120085	25.90684687130364	20.54290201857633
51	19.593957844525022	32.83659420992418	24.034360396346784	23.535087549204007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1882.0
1	2975.5
2	4069.0
3	11479.0
4	18889.0
5	13963.5
6	9038.0
7	9484.5
8	9931.0
9	10653.5
10	11376.0
11	11480.0
12	11584.0
13	11687.5
14	11791.0
15	11639.0
16	11487.0
17	10358.0
18	9229.0
19	8635.0
20	8041.0
21	9108.0
22	10175.0
23	9829.5
24	9484.0
25	10537.5
26	14277.5
27	16964.0
28	20060.0
29	23156.0
30	27257.5
31	31359.0
32	37375.0
33	43391.0
34	46701.5
35	50012.0
36	58931.0
37	67850.0
38	81868.5
39	95887.0
40	134510.0
41	173133.0
42	208602.5
43	244072.0
44	243399.0
45	242726.0
46	234619.5
47	226513.0
48	214426.5
49	202340.0
50	195069.5
51	187799.0
52	171272.0
53	154745.0
54	141556.5
55	128368.0
56	120422.0
57	112476.0
58	109549.5
59	106623.0
60	98513.0
61	90403.0
62	78495.0
63	66587.0
64	60163.0
65	53739.0
66	45956.0
67	38173.0
68	32788.5
69	27404.0
70	24373.0
71	21342.0
72	17367.5
73	13393.0
74	11887.0
75	7369.5
76	4358.0
77	4038.0
78	3718.0
79	2679.0
80	1640.0
81	1175.0
82	710.0
83	629.0
84	548.0
85	332.5
86	117.0
87	76.0
88	35.0
89	25.5
90	16.0
91	9.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2578550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.281585358382472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37144177206159	12.434846331744083
2	7.276279191572238	2.2238616311486674
3	2.42019288220492	1.1095315233949252
4	1.2847023232806727	0.785291528533035
5	0.7686840395239158	0.5873355381805483
6	0.5054777042914422	0.46347004109333334
7	0.3505168322133277	0.3749517023712456
8	0.2685438730257933	0.32830208944914274
9	0.21336404715943197	0.29344868091691223
>10	2.3479947457687786	8.701408768186564
>50	1.178866598206885	13.184764199711067
>100	1.9938778788353004	56.72559225261327
>500	0.017773010505070283	1.6849662172139674
>1k	0.001777301050507028	0.379541288525273
>5k	2.539001500724326E-4	0.293366559245511
>10k+	2.539001500724326E-4	0.42932164767247455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11065	0.4291171394776134	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7561	0.2932268135192259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8781485718717885E-5	0.0	0.0	0.019507087316515095	0.0
2	3.8781485718717885E-5	0.0	0.0	0.0713191522367222	0.0
3	3.8781485718717885E-5	0.0	0.0	0.10381803726900778	0.0
4	3.8781485718717885E-5	0.0	0.0	0.179946093734851	0.0
5	3.8781485718717885E-5	0.0	0.0	0.30734327432083924	0.0
6	7.756297143743577E-5	0.0	0.0	0.48934478679878224	0.0
7	7.756297143743577E-5	0.0	0.0	0.5846696786953908	0.0
8	7.756297143743577E-5	0.0	0.0	0.8599018828411317	0.0
9	1.1634445715615365E-4	0.0	0.0	0.9857865854840899	0.0
10	1.1634445715615365E-4	0.0	0.0	1.1968354307653526	0.0
11	1.1634445715615365E-4	0.0	0.0	1.3670085900990867	0.0
12	1.1634445715615365E-4	0.0	0.0	1.5248492369742686	0.0
13	1.5512594287487154E-4	0.0	0.0	1.5874425549242792	0.0
14	1.5512594287487154E-4	0.0	0.0	1.6114482945841655	0.0
15	1.5512594287487154E-4	0.0	0.0	1.6437144907021388	0.0
16	1.5512594287487154E-4	0.0	0.0	1.7174768765391402	0.0
17	1.939074285935894E-4	0.0	0.0	1.802990052548913	0.0
18	1.939074285935894E-4	0.0	0.0	1.9453568866223265	0.0
19	1.939074285935894E-4	0.0	0.0	1.9942991215993484	0.0
20	1.939074285935894E-4	0.0	0.0	2.0561168098349847	0.0
21	1.939074285935894E-4	0.0	0.0	2.1333307479009522	0.0
22	1.939074285935894E-4	0.0	0.0	2.2067828818522037	0.0
23	1.939074285935894E-4	0.0	0.0	2.2975315584340037	0.0
24	1.939074285935894E-4	0.0	0.0	2.3658645362703847	0.0
25	1.939074285935894E-4	0.0	0.0	2.420507649648058	0.0
26	1.939074285935894E-4	0.0	0.0	2.4754997963972	0.0
27	1.939074285935894E-4	0.0	0.0	2.5276608946888754	0.0
28	1.939074285935894E-4	0.0	0.0	2.5845921157239533	0.0
29	1.939074285935894E-4	0.0	0.0	2.6473793411025577	0.0
30	2.326889143123073E-4	0.0	0.0	2.739601714141669	0.0
31	2.326889143123073E-4	0.0	0.0	2.823563630722693	0.0
32	2.714704000310252E-4	0.0	0.0	2.8956584126737894	0.0
33	2.714704000310252E-4	0.0	0.0	2.9727947877683194	0.0
34	2.714704000310252E-4	0.0	0.0	3.0541195633204707	0.0
35	2.714704000310252E-4	0.0	0.0	3.1592173896181963	0.0
36	2.714704000310252E-4	0.0	0.0	3.2456613212852186	0.0
37	2.714704000310252E-4	0.0	0.0	3.335789494095519	0.0
38	2.714704000310252E-4	0.0	0.0	3.4161059510189835	0.0
39	2.714704000310252E-4	0.0	0.0	3.501851815943069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	25	3.8921607E-5	45.0	22
GTCGACC	20	7.034921E-4	45.0	11
TAGAACG	110	0.0	45.0	1
CCGTACA	25	3.8921607E-5	45.0	43
CTACGTC	40	6.8212103E-9	45.0	43
CGTACAC	20	7.034921E-4	45.0	15
TCGTAGC	40	6.8212103E-9	45.0	18
TCGACCA	35	1.2127202E-7	45.0	38
TATCGTG	50	2.1827873E-11	45.0	1
TAGCTAC	20	7.034921E-4	45.0	27
CAATACG	25	3.8921607E-5	45.0	1
AGTCGAT	70	0.0	45.0	10
GTACGAT	25	3.8921607E-5	45.0	13
AAATGCG	75	0.0	45.0	1
AAGTCGA	45	3.8562575E-10	45.0	17
TCTAGCG	100	0.0	42.75	1
TCACGAC	190	0.0	42.63158	25
TTCTACG	75	0.0	42.0	1
TAGTCCG	65	0.0	41.53846	1
ACGTTAG	60	3.6379788E-12	41.249996	1
>>END_MODULE