FastQCFastQC Report
Thu 26 May 2016
SRR1545499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545499_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1096030
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38120.3478006988859794No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27330.24935448847203087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATG207.033142E-445.00000415
CACGCTT207.033142E-445.00000431
TAACGTG207.033142E-445.0000041
ACGGATC207.033142E-445.0000045
ACAATCG207.033142E-445.00000442
ACTAACG207.033142E-445.0000041
CATGTCC207.033142E-445.00000427
ATGCGAA207.033142E-445.00000411
ACGTACG207.033142E-445.0000041
TATACGA207.033142E-445.00000415
TTCGCGA207.033142E-445.00000415
TCGACAG406.8139343E-945.0000041
TCGCGAA207.033142E-445.00000416
GTACGTA207.033142E-445.0000041
TACGTAC207.033142E-445.00000444
ATAGGCG351.2118653E-745.012
ACGTTAG551.8189894E-1245.01
TATCTAC502.1827873E-1145.039
GCGATAC850.045.09
GCGTAAG1000.045.01