##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545499_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1096030
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.022727480087223	31.0	31.0	33.0	30.0	34.0
2	31.300769139530853	31.0	31.0	34.0	28.0	34.0
3	31.4139083784203	31.0	31.0	34.0	28.0	34.0
4	35.19335967081193	37.0	35.0	37.0	32.0	37.0
5	35.046268806510774	37.0	35.0	37.0	32.0	37.0
6	35.10076548999571	37.0	35.0	37.0	32.0	37.0
7	35.71432168827496	37.0	35.0	37.0	35.0	37.0
8	35.7085143654827	37.0	35.0	37.0	35.0	37.0
9	37.37049533315694	39.0	37.0	39.0	35.0	39.0
10	36.59018366285594	39.0	35.0	39.0	32.0	39.0
11	36.21328613267885	38.0	35.0	39.0	31.0	39.0
12	35.28433072087443	37.0	35.0	39.0	30.0	39.0
13	34.83509666706203	37.0	33.0	39.0	27.0	39.0
14	35.72758866089432	38.0	34.0	40.0	27.0	41.0
15	36.102488982965795	38.0	34.0	40.0	30.0	41.0
16	36.296448089924546	38.0	35.0	40.0	31.0	41.0
17	36.242218734888645	37.0	34.0	40.0	31.0	41.0
18	36.18119485780499	37.0	35.0	40.0	31.0	41.0
19	36.131224510278	37.0	35.0	40.0	30.0	41.0
20	36.00488216563415	37.0	34.0	40.0	30.0	41.0
21	35.83659662600476	37.0	34.0	40.0	30.0	41.0
22	35.71322226581389	36.0	34.0	40.0	30.0	41.0
23	35.67315675665812	36.0	34.0	40.0	30.0	41.0
24	35.57228178060819	36.0	34.0	40.0	29.0	41.0
25	35.46692700017335	36.0	34.0	40.0	29.0	41.0
26	35.31350236763592	36.0	34.0	40.0	29.0	41.0
27	35.189350656460135	36.0	34.0	40.0	29.0	41.0
28	35.211960439039075	36.0	34.0	40.0	29.0	41.0
29	35.22925741083729	36.0	34.0	40.0	29.0	41.0
30	35.17603623988394	36.0	34.0	40.0	29.0	41.0
31	35.02824192768446	36.0	34.0	40.0	28.0	41.0
32	34.87490214683905	36.0	34.0	40.0	27.0	41.0
33	34.68368657792214	36.0	33.0	40.0	26.0	41.0
34	34.565583971241665	36.0	33.0	40.0	25.0	41.0
35	34.442620183754094	35.0	33.0	40.0	25.0	41.0
36	34.21801775498846	35.0	33.0	40.0	24.0	41.0
37	34.140858370665036	35.0	33.0	40.0	24.0	41.0
38	34.12805488900851	35.0	33.0	40.0	24.0	41.0
39	34.08020948331706	35.0	33.0	40.0	24.0	41.0
40	33.9110517047891	35.0	33.0	40.0	23.0	41.0
41	33.87286570623067	35.0	33.0	40.0	23.0	41.0
42	33.82719359871536	35.0	33.0	40.0	23.0	41.0
43	33.74772770818317	35.0	33.0	39.0	23.0	41.0
44	33.64460279371915	35.0	33.0	39.0	23.0	41.0
45	33.572522649927464	35.0	32.0	39.0	23.0	41.0
46	33.51982883680191	35.0	32.0	39.0	23.0	41.0
47	33.45468554692846	35.0	32.0	39.0	23.0	41.0
48	33.402905029971805	35.0	32.0	39.0	23.0	41.0
49	33.385792359698186	35.0	32.0	39.0	23.0	41.0
50	33.34035564719944	35.0	32.0	39.0	23.0	41.0
51	33.13896243715957	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	18.0
15	46.0
16	155.0
17	362.0
18	868.0
19	1643.0
20	2759.0
21	4183.0
22	6056.0
23	8592.0
24	11825.0
25	15779.0
26	20231.0
27	23482.0
28	27157.0
29	31452.0
30	37401.0
31	45098.0
32	54779.0
33	69668.0
34	112289.0
35	155412.0
36	80170.0
37	94639.0
38	122281.0
39	169558.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.29388794102351	24.76182221289563	28.03499904199703	13.90929080408383
2	29.669625831409725	26.65091283997701	30.012225942720544	13.667235385892724
3	28.75769823818691	26.04919573369342	31.325146209501565	13.867959818618104
4	25.895824019415524	28.61171683256845	30.325721011286188	15.166738136729835
5	22.60585932866801	33.29917976697718	29.57090590586024	14.524054998494568
6	21.282994078629237	41.63891499320274	26.710856454659087	10.367234473508937
7	87.7785279600011	4.6708575495196305	5.91735627674425	1.6332582137350253
8	88.78251507714204	3.5553771338375775	5.633513681194858	2.0285941078255156
9	84.00509110152095	5.639535414176619	7.51941096502833	2.8359625192741076
10	50.2119467532823	26.64616844429441	12.122478399313888	11.019406403109404
11	45.60112405682326	21.24695491911718	19.695720007664026	13.456201016395536
12	42.21709259783035	20.76512504219775	23.09781666560222	13.91996569436968
13	23.167431548406523	38.849301570212496	22.74718757698238	15.236079304398602
14	16.655292282145563	41.88617099896901	25.500031933432478	15.958504785452954
15	15.531235458883424	23.71048237730719	44.99776465972647	15.76051750408292
16	16.87727525706413	18.99263706285412	44.978695838617554	19.151391841464193
17	17.249710318148225	18.79975913068073	28.322126219172834	35.62840433199821
18	20.918131802961597	22.945083619973904	33.70227092324115	22.434513653823345
19	29.595266552922823	24.185743090973787	24.67204364844028	21.54694670766311
20	32.39199656943697	22.97619590704634	23.798162459056776	20.833645064459912
21	22.97555723839676	28.780872786328842	26.73284490388037	21.51072507139403
22	23.824804065582146	25.161537549154676	23.344434002718902	27.66922438254427
23	20.265138727954525	30.83209401202522	23.96859575011633	24.934171509903926
24	20.33593970967948	24.31521035008166	37.829986405481606	17.51886353475726
25	18.422579673914036	24.86537777250623	35.03489867978066	21.677143873799075
26	18.171035464357725	33.075645739623916	26.871436000839395	21.881882795178964
27	18.99336696988221	34.1059095097762	26.14298878680328	20.757734733538317
28	17.171883981277887	29.143727817669223	35.11765188909063	18.566736311962266
29	17.576708666733577	23.790407196883297	35.49300657828709	23.139877558096035
30	19.200204373967868	31.12998731786539	29.516710309024386	20.153097999142357
31	28.994918022316906	26.897530177093692	24.037115772378492	20.070436028210906
32	31.03163234582995	25.455690081475872	25.037909546271543	18.474768026422634
33	29.036796438053702	26.640511664826693	23.873799074842843	20.44889282227676
34	20.707736102113994	26.610859191810444	26.964407908542647	25.716996797532914
35	21.233634115854493	25.007070974334646	30.094522960137954	23.66477194967291
36	30.523799531034733	25.699935220751257	24.166218077972317	19.61004717024169
37	22.04547320785015	32.5654407269874	26.192257511199514	19.196828553962938
38	21.528699031960805	33.319617163763766	23.66586681021505	21.48581699406038
39	21.365929764696222	32.17548789722909	25.634608541737	20.82397379633769
40	25.145479594536646	26.444166674269866	24.74439568259993	23.66595804859356
41	18.304334735363085	24.543032581224967	26.76167623149001	30.390956451921937
42	21.208178608249774	26.227019333412404	23.69442442268916	28.87037763564866
43	21.724770307382098	26.396540240686843	25.730317600795598	26.14837185113546
44	20.340592866983567	29.21452879939418	28.241562730947145	22.203315602675108
45	18.646843608295395	35.404961543023454	23.017526892512066	22.93066795616908
46	20.882913788856143	33.545249673822795	24.640292692718265	20.931543844602793
47	21.590558652591625	27.2993439960585	26.384861728237365	24.725235623112507
48	23.399359506582847	23.463773801811993	30.210304462469097	22.926562229136064
49	21.13308942273478	24.549692982856307	31.866919701103075	22.45029789330584
50	20.069249929290255	32.55951023238415	25.968176053575178	21.40306378475042
51	18.726768427871498	33.89514885541454	23.957373429559407	23.420709287154548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	953.0
1	1317.5
2	1682.0
3	4195.0
4	6708.0
5	5056.5
6	3405.0
7	3701.5
8	3998.0
9	4293.0
10	4588.0
11	4748.5
12	4909.0
13	4875.0
14	4841.0
15	4764.0
16	4687.0
17	4304.5
18	3922.0
19	3951.0
20	3980.0
21	3865.0
22	3750.0
23	4112.5
24	4475.0
25	4971.0
26	5882.0
27	6297.0
28	7826.5
29	9356.0
30	12138.5
31	14921.0
32	17238.0
33	19555.0
34	22182.0
35	24809.0
36	27485.0
37	30161.0
38	35972.5
39	41784.0
40	56348.5
41	70913.0
42	90058.5
43	109204.0
44	111695.5
45	114187.0
46	106352.0
47	98517.0
48	91546.5
49	84576.0
50	80083.0
51	75590.0
52	69158.5
53	62727.0
54	58158.5
55	53590.0
56	49974.0
57	46358.0
58	43478.5
59	40599.0
60	39391.5
61	38184.0
62	33808.5
63	29433.0
64	25563.0
65	21693.0
66	18004.5
67	14316.0
68	12403.5
69	10491.0
70	9038.5
71	7586.0
72	6608.0
73	5630.0
74	4543.5
75	3026.0
76	2595.0
77	1899.0
78	1203.0
79	894.0
80	585.0
81	406.0
82	227.0
83	157.0
84	87.0
85	51.5
86	16.0
87	15.0
88	14.0
89	8.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1096030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.435003769993273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86168760806089	15.326771959706953
2	6.520346301894933	2.534459099179794
3	2.5053292612046354	1.4607325090975964
4	1.3445267677154173	1.0452353119762403
5	0.8787625667811741	0.8539376899160542
6	0.6462598126624702	0.7536037137294154
7	0.4819719925960389	0.655698924519463
8	0.37766434156076567	0.5871926321620407
9	0.33320323865261803	0.5828225579448839
>10	5.032691316831151	24.654931059384168
>50	2.25152905100501	31.320576727415624
>100	0.7636765202214154	19.428196533689697
>500	0.0014107324880937478	0.19768083272903417
>1k	9.404883253958319E-4	0.5981604485490192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3812	0.3478006988859794	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2733	0.24935448847203087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01751776867421512	0.0
2	0.0	0.0	0.0	0.05437807359287611	0.0
3	0.0	0.0	0.0	0.0858553141793564	0.0
4	0.0	0.0	0.0	0.16140069158690912	0.0
5	0.0	0.0	0.0	0.2700656003941498	0.0
6	0.0	0.0	0.0	0.45655684607173164	0.0
7	0.0	0.0	0.0	0.5602948824393493	0.0
8	0.0	0.0	0.0	0.8949572548196674	0.0
9	0.0	0.0	0.0	1.0500624982892803	0.0
10	0.0	0.0	0.0	1.2942163991861537	0.0
11	0.0	0.0	0.0	1.4620037772688703	0.0
12	0.0	0.0	0.0	1.6276014342673102	0.0
13	0.0	0.0	0.0	1.6961214565294747	0.0
14	0.0	0.0	0.0	1.7227630630548434	0.0
15	0.0	0.0	0.0	1.7536928733702544	0.0
16	0.0	0.0	0.0	1.8234902329315803	0.0
17	0.0	0.0	0.0	1.9064259189985675	0.0
18	0.0	0.0	0.0	2.038904044597319	0.0
19	0.0	0.0	0.0	2.0919135425125224	0.0
20	0.0	0.0	0.0	2.1542293550359024	0.0
21	0.0	0.0	0.0	2.2247566216253203	0.0
22	0.0	0.0	0.0	2.3037690574163117	0.0
23	0.0	0.0	0.0	2.3990219245823563	0.0
24	0.0	0.0	0.0	2.475114732260978	0.0
25	0.0	0.0	0.0	2.537430544784358	0.0
26	0.0	0.0	0.0	2.5954581535176957	0.0
27	0.0	0.0	0.0	2.6534857622510333	0.0
28	0.0	0.0	0.0	2.7170789120735748	0.0
29	0.0	0.0	0.0	2.786602556499366	0.0
30	0.0	0.0	0.0	2.8836801912356416	0.0
31	0.0	0.0	0.0	2.969900458929044	0.0
32	0.0	0.0	0.0	3.0464494585002235	0.0
33	9.123837851153709E-5	0.0	0.0	3.1286552375391183	0.0
34	1.8247675702307418E-4	0.0	0.0	3.2110434933350365	0.0
35	1.8247675702307418E-4	0.0	0.0	3.316971250786931	0.0
36	1.8247675702307418E-4	0.0	0.0	3.4077534374059106	0.0
37	1.8247675702307418E-4	0.0	0.0	3.489867978066294	0.0
38	1.8247675702307418E-4	0.0	0.0	3.5825661706340153	0.0
39	1.8247675702307418E-4	0.0	0.0	3.6758117934728065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATG	20	7.033142E-4	45.000004	15
CACGCTT	20	7.033142E-4	45.000004	31
TAACGTG	20	7.033142E-4	45.000004	1
ACGGATC	20	7.033142E-4	45.000004	5
ACAATCG	20	7.033142E-4	45.000004	42
ACTAACG	20	7.033142E-4	45.000004	1
CATGTCC	20	7.033142E-4	45.000004	27
ATGCGAA	20	7.033142E-4	45.000004	11
ACGTACG	20	7.033142E-4	45.000004	1
TATACGA	20	7.033142E-4	45.000004	15
TTCGCGA	20	7.033142E-4	45.000004	15
TCGACAG	40	6.8139343E-9	45.000004	1
TCGCGAA	20	7.033142E-4	45.000004	16
GTACGTA	20	7.033142E-4	45.000004	1
TACGTAC	20	7.033142E-4	45.000004	44
ATAGGCG	35	1.2118653E-7	45.0	12
ACGTTAG	55	1.8189894E-12	45.0	1
TATCTAC	50	2.1827873E-11	45.0	39
GCGATAC	85	0.0	45.0	9
GCGTAAG	100	0.0	45.0	1
>>END_MODULE