FastQCFastQC Report
Thu 26 May 2016
SRR1545497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545497_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2948529
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120720.4094244960792314No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC93280.31636114143696736No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT207.0350844E-445.00000428
CGCATCG650.045.00000421
CACGCTA406.8212103E-945.00000436
GCACGAC207.0350844E-445.00000437
CGCTAAG406.8212103E-945.00000438
ACGTCAT406.8212103E-945.00000435
TCGCTAA551.8189894E-1245.026
TATTACG253.8922968E-545.01
TACGTAG750.045.01
CGTTTTT111700.042.945391
TGTTACG2150.042.9069751
GCAACGA700.041.78571311
TTACGTC650.041.53846413
TAGGCGA650.041.5384645
ATCGTAG1850.041.3513531
CGTTGAT2350.041.1702125
TCACGAC4650.041.12903225
ACGTAGC556.184564E-1140.90909218
TACGGGA4350.040.862074
CGACGGT4850.040.82474528
Produced by FastQC (version 0.11.5)