##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545497_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2948529
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.018388491346023	31.0	31.0	33.0	28.0	34.0
2	31.299269567977795	31.0	31.0	34.0	28.0	34.0
3	31.412983898072564	31.0	31.0	34.0	28.0	34.0
4	35.17464335605992	37.0	35.0	37.0	32.0	37.0
5	35.027476412814664	37.0	35.0	37.0	32.0	37.0
6	35.06967711696239	37.0	35.0	37.0	32.0	37.0
7	35.722604729341306	37.0	35.0	37.0	35.0	37.0
8	35.72995890493192	37.0	35.0	37.0	35.0	37.0
9	37.4219575252609	39.0	37.0	39.0	35.0	39.0
10	36.54919453056083	39.0	35.0	39.0	32.0	39.0
11	36.19179088962666	38.0	35.0	39.0	31.0	39.0
12	35.170115335477455	37.0	34.0	39.0	30.0	39.0
13	34.68215405037563	37.0	33.0	39.0	27.0	39.0
14	35.558381145310086	37.0	33.0	40.0	27.0	41.0
15	35.97664191194999	37.0	34.0	40.0	30.0	41.0
16	36.18561357205576	37.0	34.0	40.0	31.0	41.0
17	36.14367333677234	37.0	34.0	40.0	31.0	41.0
18	36.06856673276742	37.0	34.0	40.0	30.0	41.0
19	35.992670921669756	37.0	34.0	40.0	30.0	41.0
20	35.84972608375227	36.0	34.0	40.0	30.0	41.0
21	35.68095175594339	36.0	34.0	40.0	30.0	41.0
22	35.549184695147986	36.0	34.0	40.0	30.0	41.0
23	35.528037879227234	36.0	34.0	40.0	30.0	41.0
24	35.456637530104	36.0	34.0	40.0	29.0	41.0
25	35.35451474277512	35.0	34.0	40.0	29.0	41.0
26	35.1999993895261	35.0	34.0	40.0	29.0	41.0
27	35.11144913277095	35.0	34.0	40.0	29.0	41.0
28	35.12977996824858	36.0	34.0	40.0	29.0	41.0
29	35.18163497798394	36.0	34.0	40.0	29.0	41.0
30	35.10925956638039	36.0	34.0	40.0	29.0	41.0
31	34.93835129313634	36.0	34.0	40.0	28.0	41.0
32	34.75983481932855	35.0	34.0	40.0	27.0	41.0
33	34.545470978918644	35.0	33.0	40.0	26.0	41.0
34	34.3981483648287	35.0	33.0	40.0	25.0	41.0
35	34.24222112110819	35.0	33.0	40.0	24.0	41.0
36	34.039443057877335	35.0	33.0	40.0	23.0	41.0
37	33.960174378478214	35.0	33.0	40.0	23.0	41.0
38	33.956606836832876	35.0	33.0	40.0	23.0	41.0
39	33.92167653769049	35.0	33.0	40.0	23.0	41.0
40	33.779158014047006	35.0	33.0	40.0	23.0	41.0
41	33.756590489698425	35.0	33.0	40.0	23.0	41.0
42	33.71822118758201	35.0	33.0	39.0	23.0	41.0
43	33.62324976284785	35.0	33.0	39.0	23.0	41.0
44	33.532990179170696	35.0	32.0	39.0	23.0	41.0
45	33.46557792037996	35.0	32.0	39.0	23.0	41.0
46	33.41497472129323	35.0	32.0	39.0	23.0	41.0
47	33.33711962812643	35.0	32.0	39.0	23.0	41.0
48	33.291905217822176	35.0	32.0	39.0	23.0	41.0
49	33.28680199516437	35.0	32.0	39.0	23.0	41.0
50	33.17289570494304	35.0	32.0	39.0	23.0	41.0
51	32.999494324118906	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	13.0
14	36.0
15	136.0
16	370.0
17	1126.0
18	2331.0
19	4422.0
20	7460.0
21	11486.0
22	16999.0
23	24054.0
24	33676.0
25	45546.0
26	56962.0
27	66339.0
28	74520.0
29	85834.0
30	101212.0
31	122483.0
32	151229.0
33	190698.0
34	309593.0
35	435184.0
36	205519.0
37	237043.0
38	317431.0
39	446534.0
40	288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.71778198552566	23.597834716904597	30.01422065036498	14.67016264720476
2	28.779876338336845	27.228763902271268	30.065771779758656	13.925587979633233
3	27.253759416983858	25.950329808524863	32.32923942752471	14.466671346966573
4	24.56258697133384	29.409783658224153	31.221805856411788	14.805823514030218
5	22.262592635175032	32.94120559777435	30.54797833088974	14.248223436160877
6	20.6824148583921	41.43771351748617	27.517857209476322	10.362014414645405
7	86.93816475944446	4.686133322751786	6.7774134152996295	1.59828850250413
8	88.34564625275858	3.323487745923476	6.313351505106445	2.017514496211501
9	83.77886057759649	5.143920917854293	8.168276452427634	2.908942052121583
10	51.987346910951196	22.445463483655747	14.612371117937114	10.954818487455949
11	47.72023609060654	20.946817887834918	18.277622502610626	13.055323518947922
12	41.66101130428088	21.09027925450284	23.05386855615122	14.19484088506506
13	21.097774517394946	42.61470719806385	22.663775733594616	13.623742550946591
14	15.32418368617029	43.904401143756765	26.73085460580513	14.040560564267809
15	13.997522154267433	23.036164813030496	48.30350998752259	14.662803045179478
16	15.252147765885974	18.367599572532608	47.0720484689145	19.30820419266692
17	16.020497000368657	18.544094360272528	28.989302801498646	36.446105837860166
18	21.04734259015258	21.69376662057589	35.84824839776037	21.410642391511157
19	28.693222959652083	24.2863136160438	25.63515569967262	21.385307724631502
20	31.183888644134072	22.38387345011699	25.326391566777872	21.10584633897106
21	22.065918293494825	28.786048907777406	28.6597147255462	20.488318073181578
22	23.406247657730347	24.39870864420869	25.731271423818452	26.463772274242515
23	19.39438275831779	31.035645231910557	24.383073729307053	25.186898280464597
24	19.76260026609879	23.19865261627069	38.820272752955795	18.218474364674726
25	16.603160423384	25.31353769964616	37.287949346945545	20.795352530024296
26	16.500465147197126	34.16184816225311	27.926569486004716	21.411117204545047
27	17.99602445829768	35.160176481221654	27.49181032304583	19.351988737434837
28	14.90132876427534	29.321502349137486	37.663594287185234	18.11357459940194
29	15.81859971531567	24.220552010850156	36.8395562668707	23.121292006963472
30	17.440730615164377	31.668028362617427	31.124299608380994	19.766941413837205
31	28.422783021635535	26.811911973733345	24.802571044748074	19.962733959883046
32	30.169450597230007	25.34629979898451	26.38563161495105	18.09861798883443
33	28.453713699271738	27.059018242655913	24.67789870813548	19.809369349936865
34	19.44908800286516	27.134174362877218	28.919166133349883	24.497571500907743
35	19.608659097468603	25.808089389658367	31.472405392655116	23.11084612021791
36	30.69418683011088	24.364827342719032	26.019347274522314	18.921638552647778
37	20.944036840064996	32.20650025826438	28.01681787766035	18.83264502401028
38	20.56554302162197	32.80795271133504	24.12918441704321	22.49731984999978
39	20.192306061768427	32.19612220195223	27.921889186099236	19.689682550180105
40	24.91360946424471	25.10675662338746	25.27212722004769	24.707506692320138
41	17.06389864233996	24.503303172531115	27.32976341762282	31.103034767506106
42	21.592292292190447	24.80297802734855	25.000669825529954	28.60405985493105
43	21.335113203906083	25.36247735735345	26.95971448813968	26.34269495060079
44	19.043326350190213	29.24356518114626	29.467405611408264	22.24570285725526
45	16.879637269974282	37.28954336213074	23.295277068667122	22.53554229922785
46	22.14897665920871	32.74249634309176	25.73513775852298	19.373389239176554
47	21.23411368855453	27.602713081675645	26.13923756557931	25.023935664190518
48	23.168400242968612	24.226792410724126	30.51060376207933	22.09420358422793
49	21.9698703997824	22.89256778549575	32.38133320038568	22.756228614336166
50	19.999905037393223	33.016226057128826	26.116175218218984	20.867693687258967
51	17.866230923962423	33.66933138524328	24.734944102635588	23.729493588158707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2679.0
1	3296.0
2	3913.0
3	12124.0
4	20335.0
5	15129.0
6	9923.0
7	10816.0
8	11709.0
9	12902.5
10	14096.0
11	14625.0
12	15154.0
13	15100.5
14	15047.0
15	14582.0
16	14117.0
17	13855.5
18	13594.0
19	12451.0
20	11308.0
21	12017.5
22	12727.0
23	12427.0
24	12127.0
25	14468.5
26	18141.5
27	19473.0
28	22634.5
29	25796.0
30	33470.5
31	41145.0
32	46974.5
33	52804.0
34	58646.5
35	64489.0
36	74256.0
37	84023.0
38	100762.5
39	117502.0
40	157746.0
41	197990.0
42	252604.5
43	307219.0
44	309273.5
45	311328.0
46	292803.0
47	274278.0
48	263092.0
49	251906.0
50	239241.0
51	226576.0
52	206905.0
53	187234.0
54	165713.5
55	144193.0
56	133259.0
57	122325.0
58	113736.5
59	105148.0
60	90059.5
61	74971.0
62	68660.5
63	62350.0
64	50191.0
65	38032.0
66	31001.0
67	23970.0
68	19257.5
69	14545.0
70	12277.5
71	10010.0
72	9166.5
73	8323.0
74	6439.5
75	3482.5
76	2409.0
77	1806.0
78	1203.0
79	924.5
80	646.0
81	521.0
82	396.0
83	234.0
84	72.0
85	60.0
86	48.0
87	31.0
88	14.0
89	10.5
90	7.0
91	5.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2948529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.520458633463846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61463401144323	13.317907264378755
2	7.741497894957937	2.5578619146940014
3	2.711146509917473	1.3436815129905446
4	1.4539955387505301	0.9608269260467645
5	0.8854278203938917	0.7313836839867672
6	0.5935583816952225	0.5883514014804999
7	0.46107352267481977	0.5332002240834371
8	0.3450843708102309	0.4560761658420256
9	0.2756798457931514	0.4098921739654906
>10	2.367666456418704	8.879619911162724
>50	0.8539904908731337	10.310204905655272
>100	1.673172597061479	56.585087924390656
>500	0.021218514273684846	2.2733343062071003
>1k	0.0014420349506387763	0.32426613475764626
>5k	2.0600499294839658E-4	0.31745954082906425
>10k+	2.0600499294839658E-4	0.41084600952920924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12072	0.4094244960792314	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9328	0.31636114143696736	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04256359696648736	0.0
2	0.0	0.0	0.0	0.15845189245213462	0.0
3	0.0	0.0	0.0	0.22696063019899076	0.0
4	0.0	0.0	0.0	0.36374069917575846	0.0
5	0.0	0.0	0.0	0.5833756425661745	0.0
6	0.0	0.0	0.0	0.9073337925453675	0.0
7	3.3915216706364424E-5	0.0	0.0	1.0974285821845402	0.0
8	3.3915216706364424E-5	0.0	0.0	1.63868152560141	0.0
9	3.3915216706364424E-5	0.0	0.0	1.8598426537436126	0.0
10	3.3915216706364424E-5	0.0	0.0	2.196959907804875	0.0
11	3.3915216706364424E-5	0.0	0.0	2.453189370021458	0.0
12	3.3915216706364424E-5	0.0	0.0	2.7019913997793474	0.0
13	6.783043341272885E-5	0.0	0.0	2.8002776977943915	0.0
14	6.783043341272885E-5	0.0	0.0	2.83677047097044	0.0
15	6.783043341272885E-5	0.0	0.0	2.888796413398003	0.0
16	6.783043341272885E-5	0.0	0.0	3.004209895849761	0.0
17	6.783043341272885E-5	0.0	0.0	3.133291210634184	0.0
18	6.783043341272885E-5	0.0	0.0	3.344074282464239	0.0
19	6.783043341272885E-5	0.0	0.0	3.4200782831032015	0.0
20	6.783043341272885E-5	0.0	0.0	3.508054355239511	0.0
21	6.783043341272885E-5	0.0	0.0	3.6252653441767064	0.0
22	6.783043341272885E-5	0.0	0.0	3.740916233145409	0.0
23	6.783043341272885E-5	0.0	0.0	3.877628471688764	0.0
24	6.783043341272885E-5	0.0	0.0	3.9766948196880545	0.0
25	6.783043341272885E-5	0.0	0.0	4.063483859239641	0.0
26	6.783043341272885E-5	0.0	0.0	4.150103322707696	0.0
27	6.783043341272885E-5	0.0	0.0	4.237129768776227	0.0
28	6.783043341272885E-5	0.0	0.0	4.327242499565037	0.0
29	6.783043341272885E-5	0.0	0.0	4.4170160781867835	0.0
30	1.0174565011909328E-4	0.0	0.0	4.5601382926876415	0.0
31	1.0174565011909328E-4	0.0	0.0	4.675517859922694	0.0
32	1.356608668254577E-4	0.0	0.0	4.782011640380678	0.0
33	1.356608668254577E-4	0.0	0.0	4.886741829569932	0.0
34	1.356608668254577E-4	0.0	0.0	4.998763790351053	0.0
35	1.356608668254577E-4	0.0	0.0	5.143920917854293	0.0
36	1.356608668254577E-4	0.0	0.0	5.259707467689821	0.0
37	1.356608668254577E-4	0.0	0.0	5.3762062370761825	0.0
38	2.0349130023818656E-4	0.0	0.0	5.494672089031513	0.0
39	2.0349130023818656E-4	0.0	0.0	5.616190310490418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	20	7.0350844E-4	45.000004	28
CGCATCG	65	0.0	45.000004	21
CACGCTA	40	6.8212103E-9	45.000004	36
GCACGAC	20	7.0350844E-4	45.000004	37
CGCTAAG	40	6.8212103E-9	45.000004	38
ACGTCAT	40	6.8212103E-9	45.000004	35
TCGCTAA	55	1.8189894E-12	45.0	26
TATTACG	25	3.8922968E-5	45.0	1
TACGTAG	75	0.0	45.0	1
CGTTTTT	11170	0.0	42.94539	1
TGTTACG	215	0.0	42.906975	1
GCAACGA	70	0.0	41.785713	11
TTACGTC	65	0.0	41.538464	13
TAGGCGA	65	0.0	41.538464	5
ATCGTAG	185	0.0	41.351353	1
CGTTGAT	235	0.0	41.17021	25
TCACGAC	465	0.0	41.129032	25
ACGTAGC	55	6.184564E-11	40.909092	18
TACGGGA	435	0.0	40.86207	4
CGACGGT	485	0.0	40.824745	28
>>END_MODULE