FastQCFastQC Report
Thu 26 May 2016
SRR1545496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545496_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2342255
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.44440080179143604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56070.23938469551778094No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27480.11732283632653148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC253.8920494E-545.00000413
CCCGATA302.166471E-645.00000424
TGCGCTA207.034789E-445.035
TATACGA207.034789E-445.018
CGACGGT2750.042.54545628
ACGGGTA4150.041.204825
CGTTTTT75650.040.9253121
TATTCCG556.184564E-1140.9090921
TACGATC501.0822987E-940.50000411
TACGGGT2400.040.3125044
GCGATCG451.9297659E-840.09
TAGTGCG1300.039.807691
TCGATAG800.039.3751
CGTAAGG4700.039.2553182
ATGTACG1150.039.1304321
TATTGCG1050.038.571431
GTTAGTC356.251068E-638.57142613
TCGTAAG1000.038.2500041
TTACGGG3650.038.2191773
CGGGTAC2650.038.2075466
Produced by FastQC (version 0.11.5)