FastQCFastQC Report
Thu 26 May 2016
SRR1545495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545495_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1755509
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83390.47501892613481334No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52190.29729269402777203No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22070.12571852380135903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT207.034303E-445.00000442
CGTTGTA207.034303E-445.00000436
GACGTAT351.212411E-745.00000436
CGTCTTA207.034303E-445.00000443
TATATCG351.212411E-745.00000435
TATTCGA207.034303E-445.00000440
CCCGATA207.034303E-445.00000444
ACGTTAG253.8916492E-545.01
TACGCAC253.8916492E-545.031
CCGAATA253.8916492E-545.041
ACGTACT253.8916492E-545.014
ACGTAAG551.8189894E-1245.01
TACGTAA253.8916492E-545.039
TATTACG302.166158E-644.9999961
CGCTTAT302.166158E-644.99999618
GCGTAAG1350.043.3333321
GCGATAT2900.042.6724179
CGTAAGG2850.042.631582
CGTATGG1700.042.352942
CGTTTTT61800.041.8689351
Produced by FastQC (version 0.11.5)