##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545495_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1755509
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04868730379622	31.0	31.0	33.0	30.0	34.0
2	31.3276121056628	31.0	31.0	34.0	30.0	34.0
3	31.44025294088495	31.0	31.0	34.0	30.0	34.0
4	35.210961037511055	37.0	35.0	37.0	32.0	37.0
5	35.07836986309953	37.0	35.0	37.0	32.0	37.0
6	35.12259635239694	37.0	35.0	37.0	32.0	37.0
7	35.72689630187028	37.0	35.0	37.0	35.0	37.0
8	35.71431704423048	37.0	35.0	37.0	35.0	37.0
9	37.38299034638957	39.0	37.0	39.0	35.0	39.0
10	36.61389830527784	39.0	35.0	39.0	32.0	39.0
11	36.261155596468036	38.0	35.0	39.0	31.0	39.0
12	35.35911749811593	37.0	35.0	39.0	30.0	39.0
13	34.94041898959219	37.0	34.0	39.0	28.0	39.0
14	35.86398019036074	38.0	34.0	40.0	29.0	41.0
15	36.20642730968625	38.0	35.0	40.0	30.0	41.0
16	36.3761023156247	38.0	35.0	40.0	31.0	41.0
17	36.32117579573787	38.0	35.0	40.0	31.0	41.0
18	36.228073453340315	37.0	35.0	40.0	30.0	41.0
19	36.1430929718959	37.0	35.0	40.0	30.0	41.0
20	36.00770033078725	37.0	34.0	40.0	30.0	41.0
21	35.88065284769261	37.0	34.0	40.0	30.0	41.0
22	35.756109481637516	37.0	34.0	40.0	30.0	41.0
23	35.71615924498251	36.0	34.0	40.0	30.0	41.0
24	35.611215322735454	36.0	34.0	40.0	29.0	41.0
25	35.4934021984507	36.0	34.0	40.0	29.0	41.0
26	35.2954539110879	36.0	34.0	40.0	29.0	41.0
27	35.21493823158981	36.0	34.0	40.0	29.0	41.0
28	35.23341207592784	36.0	34.0	40.0	29.0	41.0
29	35.2646565753864	36.0	34.0	40.0	29.0	41.0
30	35.20244555852462	36.0	34.0	40.0	29.0	41.0
31	35.03547518127221	36.0	34.0	40.0	27.0	41.0
32	34.88913414855748	36.0	34.0	40.0	27.0	41.0
33	34.69094091799017	36.0	33.0	40.0	26.0	41.0
34	34.547751677718544	36.0	33.0	40.0	25.0	41.0
35	34.38838023615943	35.0	33.0	40.0	25.0	41.0
36	34.201665158082356	35.0	33.0	40.0	24.0	41.0
37	34.13183014157148	35.0	33.0	40.0	23.0	41.0
38	34.11326572521132	35.0	33.0	40.0	24.0	41.0
39	34.06134232293882	35.0	33.0	40.0	23.0	41.0
40	33.88298949193653	35.0	33.0	40.0	23.0	41.0
41	33.84010734208711	35.0	33.0	40.0	23.0	41.0
42	33.793479839750184	35.0	33.0	40.0	23.0	41.0
43	33.709439826284	35.0	33.0	39.0	23.0	41.0
44	33.61851576950047	35.0	32.0	39.0	23.0	41.0
45	33.537959645891874	35.0	32.0	39.0	23.0	41.0
46	33.471607379967864	35.0	32.0	39.0	23.0	41.0
47	33.392132993906614	35.0	32.0	39.0	23.0	41.0
48	33.328428393132704	35.0	32.0	39.0	23.0	41.0
49	33.33578124635078	35.0	32.0	39.0	23.0	41.0
50	33.273902896538836	35.0	32.0	39.0	23.0	41.0
51	33.10970208640343	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	0.0
14	24.0
15	65.0
16	231.0
17	577.0
18	1320.0
19	2535.0
20	4294.0
21	6785.0
22	9715.0
23	13773.0
24	19190.0
25	25491.0
26	32748.0
27	39249.0
28	43860.0
29	50016.0
30	59326.0
31	71776.0
32	87196.0
33	110376.0
34	177246.0
35	243656.0
36	129927.0
37	152493.0
38	198383.0
39	275069.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.67462086494572	23.314035986144187	28.414778847616272	14.596564301293814
2	30.21949759300579	26.315216840244055	30.115823957610015	13.349461609140143
3	29.31047348660702	25.51527790515457	31.070817637505705	14.103430970732706
4	25.921257025740115	27.963342825357202	30.856634742402345	15.258765406500338
5	22.420619888590718	32.92321486247008	29.638241672358273	15.017923576580925
6	21.198638115782945	41.478226542843124	27.283995695835223	10.039139645538702
7	87.0457514031543	4.485422746337387	6.637789951518334	1.8310358989899795
8	87.65070415474942	3.9247306621612306	6.520160249819283	1.9044049332700659
9	82.87209008897135	5.754171582145121	8.245414862584013	3.128323466299518
10	50.26587730396142	24.579025228580427	13.68093242472696	11.474165042731196
11	45.03753612200222	21.017778889199658	19.62581792517156	14.318867063626561
12	41.45339044117689	20.006334345195608	23.67011504925352	14.87016016437398
13	22.41515138914127	37.10211682195876	24.086404569842706	16.396327219057266
14	16.659669645669716	40.27259330484777	26.59063553647404	16.477101513008478
15	16.24491814055069	22.308116905125523	45.29569486684489	16.151270087478903
16	17.659265774199962	18.116569040660004	44.94588179268805	19.27828339245199
17	18.032604788696613	18.565384740266214	28.492534074163107	34.90947639687407
18	21.13181988813501	21.945600962455902	34.42067229504377	22.501906854365313
19	29.244509711998056	23.97583834659919	25.57326678473309	21.20638515666966
20	31.79624826759646	21.6888093424756	25.357488910623644	21.15745347930429
21	23.715970695678575	26.84509165148114	27.504615470498873	21.93432218234142
22	23.939951318962194	24.882697838632556	24.22505381630057	26.95229702610468
23	20.907725337779528	29.490022551863877	24.527416265026268	25.07483584533033
24	21.225752758886454	22.528053117357985	37.77195104098014	18.474243082775423
25	20.01579029216028	23.68372933434121	34.32975849169671	21.9707218818018
26	18.516339135828982	31.167370830910006	27.451639382082348	22.86465065117866
27	18.86843075142309	32.576363892181696	27.858188138027206	20.697017218368003
28	17.598998353184175	28.290997084036597	36.00796122378182	18.102043338997408
29	18.1919317986977	23.91272274878682	35.14211547761931	22.75322997489617
30	19.900325204826636	29.899134666925658	29.410615382775024	20.78992474547268
31	27.509286480445272	26.759589384047587	24.91716077787126	20.813963357635874
32	30.18309789354541	25.725701206886438	26.091464071104163	17.999736828463995
33	29.056814861102964	26.72170863265298	24.07318902950654	20.14828747673752
34	21.107895203043675	26.93036606477096	26.979753450423782	24.981985281761588
35	20.574830433794418	24.937781577878553	30.653502773269746	23.833885215057286
36	29.19438180037812	25.26258765976136	25.305424238782027	20.23760630107849
37	22.04186933818055	31.200979317109738	27.75103972693959	19.006111617770117
38	22.25907130068829	30.94065595790167	23.912494894643093	22.887777846766948
39	21.709828887234416	30.789702587682548	25.839628278749927	21.660840246333116
40	24.843392998839654	25.414623337163185	25.8058489019424	23.936134762054767
41	18.044168386490757	24.660995756786207	27.758217132466996	29.53661872425604
42	22.067844710565428	25.6481168709474	24.775207646329356	27.508830772157815
43	21.581205223100536	25.906047761646338	26.47995538615866	26.032791629094472
44	20.571868329925966	28.76840847868054	27.108092296878	23.551630894515494
45	18.93245776581037	34.28726369389163	23.838100516716235	22.942178023581764
46	22.421360414557828	31.84324318474015	25.357830691839233	20.377565708862786
47	22.43873429301701	27.271919426217693	26.48787331765317	23.801472963112122
48	23.56951744479806	23.45291308674578	29.981902684634488	22.995666783821672
49	21.70999977784221	23.541548348655574	31.383718340378774	23.36473353312344
50	20.786051225029322	31.35478086412545	26.39957983695897	21.459588073886263
51	20.232593509916498	32.777445174020755	23.45023580055699	23.53972551550576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1637.0
1	2271.0
2	2905.0
3	8558.0
4	14211.0
5	10512.5
6	6814.0
7	7190.5
8	7567.0
9	7977.5
10	8388.0
11	8442.5
12	8497.0
13	8391.5
14	8286.0
15	7819.5
16	7353.0
17	7082.0
18	6811.0
19	6676.0
20	6541.0
21	6310.5
22	6080.0
23	6718.5
24	7357.0
25	8369.0
26	10270.5
27	11160.0
28	14185.0
29	17210.0
30	20704.5
31	24199.0
32	26868.5
33	29538.0
34	33312.0
35	37086.0
36	41123.5
37	45161.0
38	54495.0
39	63829.0
40	85315.5
41	106802.0
42	132739.5
43	158677.0
44	162037.0
45	165397.0
46	156696.5
47	147996.0
48	138990.5
49	129985.0
50	126686.5
51	123388.0
52	113931.5
53	104475.0
54	97512.0
55	90549.0
56	87015.0
57	83481.0
58	77436.5
59	71392.0
60	68787.0
61	66182.0
62	61112.5
63	56043.0
64	46681.0
65	37319.0
66	32121.0
67	26923.0
68	22570.0
69	18217.0
70	16005.5
71	13794.0
72	11967.5
73	10141.0
74	8116.0
75	5049.0
76	4007.0
77	3316.5
78	2626.0
79	1885.0
80	1144.0
81	801.0
82	458.0
83	407.0
84	356.0
85	193.0
86	30.0
87	21.5
88	13.0
89	9.0
90	5.0
91	6.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1755509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.13352315146086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78821884245042	13.841868179024107
2	6.587232637465585	2.257250057961504
3	2.2220005078053116	1.1421209142912028
4	1.200169503959209	0.8225252792704961
5	0.7809676350936611	0.669036352820944
6	0.5072603355201982	0.5214694021471873
7	0.3746649142816236	0.4493530988019008
8	0.2914916635473112	0.3995423332676557
9	0.24512552084277484	0.37798774077362257
>10	3.5890401598703923	15.69155300177964
>50	1.8666615052950295	23.46868427988266
>100	1.5418363112539368	38.935865363099914
>500	0.0036646930474919107	0.3994528581809616
>1k	9.994617402250665E-4	0.24938690159940288
>5k	6.66307826816711E-4	0.7739042370988106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8339	0.47501892613481334	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5219	0.29729269402777203	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2207	0.12571852380135903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028709622109598983	0.0
2	0.0	0.0	0.0	0.09661015694023785	0.0
3	0.0	0.0	0.0	0.14052904314361248	0.0
4	5.6963535931744014E-5	0.0	0.0	0.2065497812885038	0.0
5	5.6963535931744014E-5	0.0	0.0	0.3270846233200741	0.0
6	5.6963535931744014E-5	0.0	0.0	0.500253772552576	0.0
7	5.6963535931744014E-5	0.0	0.0	0.597034820100609	0.0
8	5.6963535931744014E-5	0.0	0.0	0.8499529196375524	0.0
9	5.6963535931744014E-5	0.0	0.0	0.9645065903962896	0.0
10	5.6963535931744014E-5	0.0	0.0	1.1489545197432767	0.0
11	1.1392707186348803E-4	0.0	0.0	1.3078827849928425	0.0
12	1.1392707186348803E-4	0.0	0.0	1.4524562391876088	0.0
13	2.8481767965872007E-4	0.0	0.0	1.5142047121376194	0.0
14	2.8481767965872007E-4	0.0	0.0	1.5411484646333342	0.0
15	2.8481767965872007E-4	0.0	0.0	1.5753835497283124	0.0
16	3.417812155904641E-4	0.0	0.0	1.6519425420205764	0.0
17	3.417812155904641E-4	0.0	0.0	1.736818210558875	0.0
18	3.417812155904641E-4	0.0	0.0	1.8809929200021192	0.0
19	3.417812155904641E-4	0.0	0.0	1.935051315601344	0.0
20	3.417812155904641E-4	0.0	0.0	1.9948060647937436	0.0
21	3.417812155904641E-4	0.0	0.0	2.0722764736609154	0.0
22	3.417812155904641E-4	0.0	0.0	2.1515127521419712	0.0
23	3.417812155904641E-4	0.0	0.0	2.2402619411236286	0.0
24	3.417812155904641E-4	0.0	0.0	2.3126056317569432	0.0
25	3.417812155904641E-4	0.0	0.0	2.369512204152756	0.0
26	3.417812155904641E-4	0.0	0.0	2.427102338979749	0.0
27	3.417812155904641E-4	0.0	0.0	2.4908445356873705	0.0
28	3.417812155904641E-4	0.0	0.0	2.54963090476893	0.0
29	4.557082874539521E-4	0.0	0.0	2.613600955620279	0.0
30	5.126718233856961E-4	0.0	0.0	2.7018374727785504	0.0
31	5.126718233856961E-4	0.0	0.0	2.7831814020890806	0.0
32	5.126718233856961E-4	0.0	0.0	2.8568922175847575	0.0
33	5.126718233856961E-4	0.0	0.0	2.9279257468916424	0.0
34	5.696353593174401E-4	0.0	0.0	3.0022061977466366	0.0
35	5.696353593174401E-4	0.0	0.0	3.1086140828671343	0.0
36	5.696353593174401E-4	0.0	0.0	3.1901858663213916	0.0
37	5.696353593174401E-4	0.0	0.0	3.272953884030216	0.0
38	5.696353593174401E-4	0.0	0.0	3.350310365825524	0.0
39	5.696353593174401E-4	0.0	0.0	3.4290339724831944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCAT	20	7.034303E-4	45.000004	42
CGTTGTA	20	7.034303E-4	45.000004	36
GACGTAT	35	1.212411E-7	45.000004	36
CGTCTTA	20	7.034303E-4	45.000004	43
TATATCG	35	1.212411E-7	45.000004	35
TATTCGA	20	7.034303E-4	45.000004	40
CCCGATA	20	7.034303E-4	45.000004	44
ACGTTAG	25	3.8916492E-5	45.0	1
TACGCAC	25	3.8916492E-5	45.0	31
CCGAATA	25	3.8916492E-5	45.0	41
ACGTACT	25	3.8916492E-5	45.0	14
ACGTAAG	55	1.8189894E-12	45.0	1
TACGTAA	25	3.8916492E-5	45.0	39
TATTACG	30	2.166158E-6	44.999996	1
CGCTTAT	30	2.166158E-6	44.999996	18
GCGTAAG	135	0.0	43.333332	1
GCGATAT	290	0.0	42.672417	9
CGTAAGG	285	0.0	42.63158	2
CGTATGG	170	0.0	42.35294	2
CGTTTTT	6180	0.0	41.868935	1
>>END_MODULE