FastQCFastQC Report
Thu 26 May 2016
SRR1545494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545494_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1745736
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.5163438228918921No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42200.24173185407186426No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24000.13747783169963843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18420.1055142358294725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG302.1661508E-645.0000041
CTTCGCA551.8189894E-1245.026
TCCGCGT207.034294E-445.032
GTCGATA207.034294E-445.038
CGACGAA253.8916412E-545.011
ATAGCCG253.8916412E-545.01
CGTTCGC207.034294E-445.042
CGCTAGC207.034294E-445.035
TATTACG207.034294E-445.01
TCCGTAC207.034294E-445.043
ACTCGAT253.8916412E-545.033
ACGTATA700.045.013
ATCGGCG207.034294E-445.010
CGGTAAG253.8916412E-545.01
CGATCGA253.8916412E-545.010
CGATATC207.034294E-445.010
TATCCGT253.8916412E-545.030
CGTTTTT66150.042.9591831
TGCGAAG1450.041.896551
TAGCACG700.041.7857131
Produced by FastQC (version 0.11.5)