FastQCFastQC Report
Thu 26 May 2016
SRR1545493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545493_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3441548
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184360.5356891724305457No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC72400.21037044957676024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG351.2128658E-745.0000041
CGTTTTT150650.043.1032181
TTGTCGA501.0822987E-940.517
CGTTAGG5250.040.2857172
TACGGGA13650.040.219784
TCGATAG1400.040.1785741
AGGGCGA32100.039.883186
TATTGCG1700.039.7058831
CGCTATA403.4609002E-739.37537
TACGTAC403.4609002E-739.37534
ACGCGCC403.4609002E-739.37528
TAACGGG9800.038.801023
CGGTCTA2150.038.72093231
TCGACGA356.2519193E-638.5714316
AATACGC356.2519193E-638.5714331
GCGTAAG2800.038.571431
CGTAAGG5700.038.2894742
CGAGGGA22950.038.2352944
GCGCGAC4550.038.0769239
TAGCGGG21800.037.775233
Produced by FastQC (version 0.11.5)