##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545493_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3441548
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09220530993611	31.0	31.0	33.0	30.0	34.0
2	31.37196081530753	31.0	31.0	34.0	30.0	34.0
3	31.466672264922646	31.0	31.0	34.0	30.0	34.0
4	35.23049017477019	37.0	35.0	37.0	33.0	37.0
5	35.08496467287395	37.0	35.0	37.0	32.0	37.0
6	35.148022634000746	37.0	35.0	37.0	32.0	37.0
7	35.79934233083485	37.0	35.0	37.0	35.0	37.0
8	35.81343627925573	37.0	35.0	37.0	35.0	37.0
9	37.48568405845277	39.0	37.0	39.0	35.0	39.0
10	36.654110301527105	39.0	35.0	39.0	32.0	39.0
11	36.23715229309602	38.0	35.0	39.0	31.0	39.0
12	35.67513804834336	37.0	35.0	39.0	30.0	39.0
13	35.40633982149893	37.0	35.0	39.0	30.0	39.0
14	36.36817589061666	38.0	35.0	40.0	30.0	41.0
15	36.625717845574144	38.0	35.0	40.0	31.0	41.0
16	36.74371213186624	38.0	35.0	40.0	31.0	41.0
17	36.672699901323476	38.0	35.0	40.0	31.0	41.0
18	36.5545042521563	38.0	35.0	40.0	31.0	41.0
19	36.50355915419457	38.0	35.0	40.0	31.0	41.0
20	36.382972720415346	38.0	35.0	40.0	31.0	41.0
21	36.25737487897888	38.0	34.0	40.0	30.0	41.0
22	36.14791309027217	38.0	34.0	40.0	30.0	41.0
23	36.08803567464408	38.0	34.0	40.0	30.0	41.0
24	36.01035057479948	37.0	34.0	40.0	30.0	41.0
25	35.908136396760995	37.0	34.0	40.0	30.0	41.0
26	35.773786970281975	37.0	34.0	40.0	30.0	41.0
27	35.685687370915645	37.0	34.0	40.0	29.0	41.0
28	35.67737250795282	37.0	34.0	40.0	29.0	41.0
29	35.69379302569658	37.0	34.0	40.0	29.0	41.0
30	35.6042989956845	37.0	34.0	40.0	29.0	41.0
31	35.45784455134724	37.0	34.0	40.0	29.0	41.0
32	35.29137992554513	37.0	34.0	40.0	28.0	41.0
33	35.064110685075434	37.0	34.0	40.0	27.0	41.0
34	34.90319559686513	37.0	34.0	40.0	26.0	41.0
35	34.743718814905385	37.0	33.0	40.0	25.0	41.0
36	34.572152705701036	37.0	33.0	40.0	24.0	41.0
37	34.53102382997419	37.0	33.0	40.0	24.0	41.0
38	34.48486785597644	37.0	33.0	40.0	24.0	41.0
39	34.437915438052876	37.0	33.0	40.0	24.0	41.0
40	34.32280880580483	36.0	33.0	40.0	23.0	41.0
41	34.26954265929169	36.0	33.0	40.0	23.0	41.0
42	34.21468653059612	36.0	33.0	40.0	23.0	41.0
43	34.17483411534577	36.0	33.0	40.0	23.0	41.0
44	34.074790472194486	36.0	33.0	40.0	23.0	41.0
45	33.99943513790887	36.0	33.0	40.0	23.0	41.0
46	33.94492914235106	36.0	33.0	40.0	23.0	41.0
47	33.87970151803781	36.0	33.0	40.0	23.0	41.0
48	33.80976670963183	35.0	33.0	40.0	23.0	41.0
49	33.78794048492132	36.0	33.0	40.0	23.0	41.0
50	33.745748424836734	35.0	33.0	40.0	23.0	41.0
51	33.590060344937804	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	7.0
13	15.0
14	33.0
15	142.0
16	408.0
17	1059.0
18	2387.0
19	4635.0
20	7774.0
21	12122.0
22	17847.0
23	25170.0
24	35228.0
25	48470.0
26	62127.0
27	71553.0
28	78648.0
29	89852.0
30	106389.0
31	129073.0
32	158849.0
33	200763.0
34	304854.0
35	411122.0
36	266135.0
37	316852.0
38	432560.0
39	657073.0
40	394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.68658406042862	24.07646210368125	29.144239743278316	15.092714092611814
2	30.79916363217947	26.693830799396085	29.46319505059932	13.043810517825118
3	28.345529395492957	25.348447849630457	32.62726540498636	13.678757349890224
4	24.95098136071326	28.318361388537948	31.86414950481586	14.866507745932935
5	22.33277002093244	33.28275531824632	30.829033911484018	13.555440749337217
6	20.827429982089456	41.515387842912546	27.205635371059767	10.451546803938228
7	87.97023897385712	3.5912327824571966	7.0664712507278695	1.3720569929578201
8	89.0708774074922	2.8812034584437	6.554841019215772	1.4930781148483183
9	84.07731055908562	5.573015398884455	8.142789233217146	2.2068848088127786
10	43.851574930816014	34.0308779653807	13.04802373815504	9.06952336564825
11	35.4194101026631	22.00518487610808	28.357123015573226	14.218282005655594
12	33.86243632225964	22.40651590505203	28.283493358221357	15.447554414466977
13	21.269847173423123	34.710543046326826	28.62055098461506	15.399058795634987
14	16.819640464116727	37.270088925099984	29.691522535789126	16.21874807499416
15	16.00358327124887	25.17544430587631	42.851269254416906	15.969703168457915
16	18.36609572204136	22.28247288720076	41.71750619198105	17.63392519877683
17	19.00586596496693	20.92526386381942	31.375764626848152	28.693105544365498
18	21.35175798797518	23.67699070302085	35.03074779140085	19.940503517603126
19	26.956997258210553	24.74066902452036	28.930934567816575	19.371399149452515
20	28.77414465815964	24.29441634985187	27.924701326263644	19.00673766572484
21	22.867325982377697	27.973661852166526	30.07507086927162	19.083941296184157
22	23.015021147460388	24.79901486191679	28.986461906095744	23.19950208452708
23	19.994839531513144	29.551498337376085	28.371186454467583	22.082475676643185
24	19.179450642559686	25.273626867909442	37.695653235113966	17.851269254416906
25	18.099820197190333	26.87680078848239	35.31375997080384	19.70961904352344
26	18.208724678545817	32.21669434800851	29.903026196351178	19.671554777094492
27	18.620370833125094	32.31734672885573	29.94864520268205	19.113637235337123
28	16.85805341084884	28.264461225006887	36.98158503092213	17.89590033322214
29	17.644908628326554	25.249451700223275	36.465625352312394	20.640014319137784
30	19.089549237726743	29.747747234674627	32.071992022194664	19.090711505403963
31	25.37930605646064	27.28455334634298	27.99182228462308	19.344318312573293
32	26.853468264862208	26.548547339743628	28.720273551320513	17.877710844073654
33	25.447414942345713	28.251443827022026	27.443667791354354	18.857473439277904
34	19.742540275480685	27.869435498211853	30.121067612597585	22.266956613709876
35	20.37280898014498	27.140374040983883	31.429810073838865	21.05700690503227
36	26.28936164772364	27.123986066735085	28.034942415447933	18.55170987009334
37	21.021383400725487	31.553330071235386	29.070290462315214	18.354996065723913
38	20.59561569386799	32.159597948365096	27.12703701938779	20.117749338379124
39	20.828214512771577	31.218044903049442	27.839390878755722	20.11434970542326
40	23.34795272359996	27.197731950854674	27.697390825291407	21.756924500253955
41	18.494410073606414	26.37807172818743	28.839231648083945	26.288286550122216
42	20.920789133262126	27.160568441875576	27.062502106610165	24.856140318252134
43	21.23849500283012	27.882046102509683	27.875450233441462	23.00400866121873
44	20.23490592024287	29.731678884037066	29.362775123287545	20.670640072432523
45	18.68211630347739	33.965384181769366	26.178742821544258	21.173756693208986
46	20.444753349364877	32.387809206787175	27.514130269285797	19.653307174562144
47	21.12613277513491	28.227501112871302	28.21146181892567	22.43490429306812
48	22.015238491516026	26.09096255522224	31.05712894313838	20.836670010123353
49	20.4830791260212	26.40741898703723	31.51006465695088	21.599437229990688
50	19.67085741648816	31.458982992537077	28.43229267759741	20.437866913377352
51	19.03373133252827	32.402017929141195	26.77443406281127	21.789816675519273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2891.0
1	3518.0
2	4145.0
3	18400.0
4	32655.0
5	24334.5
6	16014.0
7	16464.0
8	16914.0
9	17861.5
10	18809.0
11	19231.5
12	19654.0
13	19564.0
14	19474.0
15	19328.5
16	19183.0
17	17365.0
18	15547.0
19	15785.5
20	16024.0
21	15812.0
22	15600.0
23	19861.0
24	24122.0
25	25945.0
26	32779.5
27	37791.0
28	47740.5
29	57690.0
30	66509.0
31	75328.0
32	83339.5
33	91351.0
34	109522.0
35	127693.0
36	134275.5
37	140858.0
38	156681.0
39	172504.0
40	208898.0
41	245292.0
42	278292.5
43	311293.0
44	325430.0
45	339567.0
46	320062.0
47	300557.0
48	275057.5
49	249558.0
50	234320.5
51	219083.0
52	202378.5
53	185674.0
54	169062.0
55	152450.0
56	136967.5
57	121485.0
58	108191.0
59	94897.0
60	87420.5
61	79944.0
62	71588.5
63	63233.0
64	51673.0
65	40113.0
66	35451.0
67	30789.0
68	24345.0
69	17901.0
70	15680.5
71	13460.0
72	12124.0
73	10788.0
74	8311.5
75	4834.0
76	3833.0
77	2867.5
78	1902.0
79	1452.0
80	1002.0
81	799.5
82	597.0
83	373.0
84	149.0
85	97.0
86	45.0
87	45.5
88	46.0
89	39.5
90	33.0
91	18.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3441548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.21549898234374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2164607573745	13.809663985357973
2	7.16054813596523	2.465448182954655
3	2.5441893131431685	1.313984655939181
4	1.3362259246292827	0.9201518418254695
5	0.8607551181534226	0.7409164430308705
6	0.6123613255677731	0.6325263466283195
7	0.4469702381474491	0.5386370975975744
8	0.3626925732796111	0.49951469009590205
9	0.3030356653482464	0.46952191695749335
>10	3.3109676056011192	13.434779232931996
>50	1.247928398092898	15.518506338222007
>100	1.586832355066991	47.61610471355897
>500	0.010014196741294184	1.0971029898208393
>1k	6.789285926301141E-4	0.1928827794121174
>5k	1.6973214815752853E-4	0.2115545103686911
>10k+	1.6973214815752853E-4	0.5387042752979543
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18436	0.5356891724305457	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7240	0.21037044957676024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024611018065126507	0.0
2	0.0	0.0	0.0	0.08853574031220834	0.0
3	0.0	0.0	0.0	0.13659550876524168	0.0
4	0.0	0.0	0.0	0.20139193177023829	0.0
5	0.0	0.0	0.0	0.33089760770444	0.0
6	0.0	0.0	0.0	0.5397280526088841	0.0
7	0.0	0.0	0.0	0.6507827291672236	0.0
8	2.9056691930491745E-5	0.0	0.0	0.9418726689268899	0.0
9	2.9056691930491745E-5	0.0	0.0	1.080211579207961	0.0
10	2.9056691930491745E-5	0.0	0.0	1.2926741106037167	0.0
11	2.9056691930491745E-5	0.0	0.0	1.4516723288473676	0.0
12	2.9056691930491745E-5	0.0	0.0	1.5863500959451968	0.0
13	2.9056691930491745E-5	0.0	0.0	1.6500423646568347	0.0
14	2.9056691930491745E-5	0.0	0.0	1.6790990565873265	0.0
15	2.9056691930491745E-5	0.0	0.0	1.7137346333684726	0.0
16	2.9056691930491745E-5	0.0	0.0	1.789340145771612	0.0
17	2.9056691930491745E-5	0.0	0.0	1.8799679679028158	0.0
18	2.9056691930491745E-5	0.0	0.0	2.01790008449686	0.0
19	5.811338386098349E-5	0.0	0.0	2.0805753689909308	0.0
20	5.811338386098349E-5	0.0	0.0	2.151502753993261	0.0
21	5.811338386098349E-5	0.0	0.0	2.237278108572073	0.0
22	8.717007579147524E-5	0.0	0.0	2.3353735005294127	0.0
23	8.717007579147524E-5	0.0	0.0	2.445876099941073	0.0
24	8.717007579147524E-5	0.0	0.0	2.5328427788890346	0.0
25	8.717007579147524E-5	0.0	0.0	2.6035377103559214	0.0
26	8.717007579147524E-5	0.0	0.0	2.676847744096552	0.0
27	1.1622676772196698E-4	0.0	0.0	2.755213642233088	0.0
28	1.1622676772196698E-4	0.0	0.0	2.8351776584258013	0.0
29	1.1622676772196698E-4	0.0	0.0	2.9226964145204426	0.0
30	1.1622676772196698E-4	0.0	0.0	3.0484247205036805	0.0
31	1.452834596524587E-4	0.0	0.0	3.1489318178912513	0.0
32	1.452834596524587E-4	0.0	0.0	3.2453128650246925	0.0
33	1.452834596524587E-4	0.0	0.0	3.340502587788983	0.0
34	1.452834596524587E-4	0.0	0.0	3.4422591229295656	0.0
35	1.452834596524587E-4	0.0	0.0	3.571212721717088	0.0
36	1.452834596524587E-4	0.0	0.0	3.6840398564831873	0.0
37	1.452834596524587E-4	0.0	0.0	3.7976805786233405	0.0
38	1.452834596524587E-4	0.0	0.0	3.912803192051949	0.0
39	1.452834596524587E-4	0.0	0.0	4.039868105863989	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	35	1.2128658E-7	45.000004	1
CGTTTTT	15065	0.0	43.103218	1
TTGTCGA	50	1.0822987E-9	40.5	17
CGTTAGG	525	0.0	40.285717	2
TACGGGA	1365	0.0	40.21978	4
TCGATAG	140	0.0	40.178574	1
AGGGCGA	3210	0.0	39.88318	6
TATTGCG	170	0.0	39.705883	1
CGCTATA	40	3.4609002E-7	39.375	37
TACGTAC	40	3.4609002E-7	39.375	34
ACGCGCC	40	3.4609002E-7	39.375	28
TAACGGG	980	0.0	38.80102	3
CGGTCTA	215	0.0	38.720932	31
TCGACGA	35	6.2519193E-6	38.57143	16
AATACGC	35	6.2519193E-6	38.57143	31
GCGTAAG	280	0.0	38.57143	1
CGTAAGG	570	0.0	38.289474	2
CGAGGGA	2295	0.0	38.235294	4
GCGCGAC	455	0.0	38.076923	9
TAGCGGG	2180	0.0	37.77523	3
>>END_MODULE