FastQCFastQC Report
Thu 26 May 2016
SRR1545492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545492_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences119301
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15491.298396492904502No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT1330.11148272017837235No Hit
GAGACATGGAATGCTTAGTAATCTTATTTGCCTGTCTTTGTCTTTGGTCTC1290.10812985641360927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGG302.1491232E-645.0000042
GCGATAG302.1491232E-645.0000049
TGAACGG207.007866E-445.02
AATGGGC207.007866E-445.04
GTAGGGT351.1997327E-745.04
ATAGAAC207.007866E-445.037
CACGAGC253.8697504E-545.041
TACGGGA253.8697504E-545.04
TAACGGG207.007866E-445.03
CGACAGG207.007866E-445.02
TATGCCA253.8697504E-545.010
TCGATCA253.8697504E-545.017
ACGGGAT406.726623E-945.05
GTAATCT207.007866E-445.018
CTACGGG207.007866E-445.03
AGCATAT453.783498E-1045.031
CAAGGGT207.007866E-445.04
CCATAGC207.007866E-445.035
CGGGATC351.1997327E-745.06
GGAGTGC253.8697504E-545.08