##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545492_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	119301
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008843178179564	31.0	31.0	33.0	28.0	34.0
2	31.323283124198458	31.0	31.0	34.0	30.0	34.0
3	31.349561193954788	31.0	31.0	34.0	28.0	34.0
4	35.18247961039723	37.0	35.0	37.0	32.0	37.0
5	35.0855231724797	37.0	35.0	37.0	32.0	37.0
6	35.13701477774704	37.0	35.0	37.0	32.0	37.0
7	35.71017845617388	37.0	35.0	37.0	35.0	37.0
8	35.68073193015985	37.0	35.0	37.0	35.0	37.0
9	37.312989832440635	39.0	37.0	39.0	35.0	39.0
10	36.6077736146386	39.0	35.0	39.0	32.0	39.0
11	36.3314808761033	38.0	35.0	39.0	32.0	39.0
12	35.91538210073679	37.0	35.0	39.0	31.0	39.0
13	35.76353928299008	38.0	35.0	39.0	30.0	39.0
14	36.80653976077317	39.0	35.0	41.0	31.0	41.0
15	36.95870948273694	39.0	35.0	41.0	31.0	41.0
16	36.979455327281414	39.0	35.0	41.0	31.0	41.0
17	36.891836614948744	38.0	35.0	41.0	31.0	41.0
18	36.68399259017108	38.0	35.0	40.0	31.0	41.0
19	36.48893135849658	38.0	35.0	40.0	30.0	41.0
20	36.29102019262202	38.0	35.0	40.0	30.0	41.0
21	36.14284037853832	38.0	34.0	40.0	30.0	41.0
22	36.076051332344235	38.0	34.0	40.0	30.0	41.0
23	35.99720035875642	37.0	34.0	40.0	30.0	41.0
24	35.873127635141365	37.0	34.0	40.0	30.0	41.0
25	35.748593892758656	37.0	34.0	40.0	30.0	41.0
26	35.62217416450826	37.0	34.0	40.0	29.0	41.0
27	35.51353299637052	37.0	34.0	40.0	29.0	41.0
28	35.422050108548966	37.0	34.0	40.0	28.0	41.0
29	35.31982967452075	37.0	34.0	40.0	27.0	41.0
30	35.1633096118222	37.0	34.0	40.0	27.0	41.0
31	34.92646331547933	37.0	33.0	40.0	25.0	41.0
32	34.5981257491555	37.0	33.0	40.0	24.0	41.0
33	34.1919598326921	37.0	33.0	40.0	22.0	41.0
34	33.77278480482142	37.0	33.0	40.0	20.0	41.0
35	33.460834360147864	36.0	32.0	40.0	18.0	41.0
36	33.26963730396225	36.0	32.0	40.0	18.0	41.0
37	33.193049513415644	36.0	32.0	40.0	18.0	41.0
38	33.089437640925055	36.0	32.0	40.0	17.0	41.0
39	32.996948893974064	36.0	31.0	40.0	16.0	41.0
40	32.87077224834662	36.0	31.0	40.0	15.0	41.0
41	32.74371547598092	36.0	31.0	40.0	15.0	41.0
42	32.665006999103106	35.0	31.0	40.0	15.0	41.0
43	32.576021994786295	35.0	31.0	40.0	15.0	41.0
44	32.42316493575075	35.0	31.0	40.0	14.0	41.0
45	32.36412100485327	35.0	31.0	40.0	14.0	41.0
46	32.29845516802038	35.0	31.0	39.0	14.0	41.0
47	32.201926220232856	35.0	30.0	39.0	14.0	41.0
48	32.11700656323082	35.0	30.0	39.0	13.0	41.0
49	32.04248078389955	35.0	30.0	39.0	13.0	41.0
50	31.98088029438144	35.0	30.0	39.0	12.0	41.0
51	31.85614538017284	35.0	30.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	6.0
16	18.0
17	54.0
18	127.0
19	263.0
20	394.0
21	652.0
22	916.0
23	1343.0
24	1796.0
25	2537.0
26	3162.0
27	3755.0
28	3658.0
29	3622.0
30	3907.0
31	4587.0
32	5366.0
33	6534.0
34	9183.0
35	11591.0
36	9766.0
37	11569.0
38	14833.0
39	19648.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.35346728024074	22.496039429677875	24.95201213736683	21.198481152714564
2	38.2125883270048	23.869037141348354	25.292327809490278	12.62604672215656
3	27.308237148054083	24.11547262805844	35.44060820948693	13.13568201440055
4	24.13475159470583	26.310760178037068	34.97036906647891	14.5841191607782
5	21.549693632073495	29.512745073385805	34.71471320441572	14.222848090124979
6	19.286510590858416	38.57888869330517	32.10869984325362	10.025900872582795
7	80.16026688795567	3.8088532367708567	14.20356912347759	1.8273107517958778
8	80.38742340801838	3.459317189294306	14.137350064123519	2.0159093385638007
9	75.3078348044023	5.650413659566977	15.828869833446493	3.21288170258422
10	37.03992422527892	28.622559743841208	22.119680472083218	12.217835558796656
11	30.916756774880344	23.94112371229076	30.284741955222504	14.857377557606391
12	28.63680941484145	21.179202186067176	34.01899397322738	16.16499442586399
13	22.14315051843656	27.139755743874737	33.77507313434087	16.942020603347835
14	18.796154265261816	28.679558427842185	34.90834108683079	17.61594622006521
15	18.128934376073964	22.96124927703875	41.87978306971442	17.030033277172866
16	19.87745282939791	21.699734285546644	40.090192035272125	18.33262084978332
17	20.468395067937404	20.82882792264943	34.93767864477247	23.7650983646407
18	22.32504337767496	22.067711083729392	36.32157316367843	19.285672374917226
19	25.365252596373878	23.751686909581647	32.26628444019749	18.61677605384699
20	26.368597077979228	23.535427196754426	31.67953328136395	18.416442443902397
21	23.673732827050905	24.289821543826122	34.161490683229815	17.874954945893162
22	22.6117132295622	23.075246645040696	32.70383316149906	21.60920696389804
23	20.66034651847009	25.414707336904137	32.88404958885508	21.040896555770697
24	20.22531244499208	23.306594244809347	37.8588611998223	18.609232110376276
25	20.36948558687689	24.379510649533533	35.34253694436761	19.908466819221967
26	19.169998575032903	27.365235832055053	32.56636574714378	20.898399845768267
27	19.07276552585477	25.893328639324064	34.523599969824225	20.51030586499694
28	18.98559106797093	25.33675325437339	36.57471437791804	19.102941299737637
29	19.458344858802526	23.95369695140862	36.528612501152544	20.059345688636306
30	20.29907544781687	25.782684135086882	35.03239704612702	18.88584337096923
31	23.827964560230004	25.141448940075943	31.964526701368808	19.066059798325245
32	24.98637899095565	24.81286829112916	31.317423994769534	18.883328723145656
33	24.35604060318019	25.38034048331531	30.26797763639869	19.995641277105808
34	21.104600967301195	26.377817453332327	31.24701385570951	21.27056772365697
35	21.146511764360735	26.73992673992674	31.605770278539158	20.50779121717337
36	24.8095154273644	26.93858391798895	28.301523038365143	19.950377616281507
37	21.86402461002003	29.342587237324082	29.325822918500265	19.46756523415562
38	22.201825634319913	28.998918701435866	28.449049044014718	20.350206620229503
39	21.647764897192815	28.63764763078264	28.5144298874276	21.200157584596944
40	23.553029731519434	26.130543750681053	29.153988650556155	21.16243786724336
41	20.16412268128515	26.072706850738886	29.92347088456928	23.839699583406677
42	21.620103771133518	26.76507321816246	28.158188112421524	23.4566348982825
43	21.27308237148054	26.75920570657413	29.251221699734288	22.716490222211046
44	21.19345185706742	27.52114399711654	29.860604688979976	21.42479945683607
45	20.11550615669609	29.928500180216428	28.073528302361257	21.88246536072623
46	21.05598444271213	29.43982028650221	28.483415897603543	21.02077937318212
47	21.221112983126712	27.696331128825406	29.42976169520792	21.65279419283996
48	20.965457121063526	26.930201758577045	30.83544982858484	21.268891291774587
49	21.604177668250895	26.529534538687855	30.42556223334255	21.440725559718697
50	20.24459141163947	29.3224700547355	29.615007418211082	20.817931115413955
51	20.03084634663582	29.454908173443645	28.80026152337365	21.713983956546887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	113.0
1	164.5
2	216.0
3	1502.5
4	2789.0
5	2054.0
6	1319.0
7	1336.5
8	1354.0
9	1408.0
10	1462.0
11	1439.0
12	1416.0
13	1360.5
14	1305.0
15	1285.5
16	1266.0
17	1187.0
18	1108.0
19	1054.5
20	1001.0
21	982.5
22	964.0
23	945.0
24	926.0
25	945.0
26	1152.0
27	1340.0
28	1437.0
29	1534.0
30	1792.5
31	2051.0
32	2358.5
33	2666.0
34	3128.0
35	3590.0
36	3735.5
37	3881.0
38	4354.5
39	4828.0
40	5502.0
41	6176.0
42	6752.5
43	7329.0
44	7534.0
45	7739.0
46	7774.5
47	7810.0
48	7579.5
49	7349.0
50	7238.0
51	7127.0
52	6939.5
53	6752.0
54	6256.0
55	5760.0
56	5559.5
57	5359.0
58	5155.0
59	4951.0
60	4588.0
61	4225.0
62	3821.5
63	3418.0
64	3046.0
65	2674.0
66	2305.5
67	1937.0
68	1726.0
69	1515.0
70	1331.5
71	1148.0
72	1005.5
73	863.0
74	687.0
75	394.0
76	277.0
77	217.5
78	158.0
79	111.0
80	64.0
81	45.5
82	27.0
83	22.5
84	18.0
85	14.0
86	10.0
87	9.5
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	119301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.97013436601537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07917576273894	46.01805517137325
2	13.560247058197113	17.07781158582074
3	6.565145625898515	12.402243065858627
4	3.321175656248336	8.36539509308388
5	1.690538309994143	5.322671226561386
6	0.8386134923592994	3.1684562577011093
7	0.39135296310100637	1.7250484069706036
8	0.19035195144028538	0.9589190367222404
9	0.12246419253500879	0.6940427993059572
>10	0.2276236622118098	2.3620925222755886
>50	0.009317927692881103	0.3872557648301355
>100	0.0026622650551088867	0.21961257659198163
>500	0.0	0.0
>1k	0.0013311325275544433	1.298396492904502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1549	1.298396492904502	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	133	0.11148272017837235	No Hit
GAGACATGGAATGCTTAGTAATCTTATTTGCCTGTCTTTGTCTTTGGTCTC	129	0.10812985641360927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0192789666473877	0.0
2	0.0	0.0	0.0	0.06286619558930771	0.0
3	0.0	0.0	0.0	0.1181884477078985	0.0
4	0.0	0.0	0.0	0.1592610288262462	0.0
5	0.0	0.0	0.0	0.24978835047484932	0.0
6	0.0	0.0	0.0	0.46940092706683095	0.0
7	0.0	0.0	0.0	0.5909422385394926	0.0
8	0.0	0.0	0.0	0.8130694629550465	0.0
9	0.0	0.0	0.0	0.9471840135455696	0.0
10	0.0	0.0	0.0	1.1056068264306251	0.0
11	0.0	0.0	0.0	1.214574898785425	0.0
12	0.0	0.0	0.0	1.305940436375219	0.0
13	0.0	0.0	0.0	1.3671302000821453	0.0
14	0.0	0.0	0.0	1.4123938609064468	0.0
15	0.0	0.0	0.0	1.4400549869657422	0.0
16	0.0	0.0	0.0	1.4819657840252807	0.0
17	0.0	0.0	0.0	1.5389644680262529	0.0
18	0.0	0.0	0.0	1.6144039027334223	0.0
19	0.0	0.0	0.0	1.6487707563222438	0.0
20	0.0	0.0	0.0	1.6948726330877362	0.0
21	0.0	0.0	0.0	1.7309159185589391	0.0
22	0.0	0.0	0.0	1.7837235228539576	0.0
23	0.0	0.0	0.0	1.8398839909137392	0.0
24	0.0	0.0	0.0	1.8968826749147116	0.0
25	0.0	0.0	0.0	1.9379552560330593	0.0
26	0.0	0.0	0.0	1.986571780622124	0.0
27	0.0	0.0	0.0	2.040217600858333	0.0
28	0.0	0.0	0.0	2.0905105573297793	0.0
29	0.0	0.0	0.0	2.124877410918601	0.0
30	0.0	0.0	0.0	2.214566516626013	0.0
31	0.0	0.0	0.0	2.272403416568176	0.0
32	0.0	0.0	0.0	2.335269612157484	0.0
33	0.0	0.0	0.0	2.411547262805844	0.0
34	0.0	0.0	0.0	2.495368856924921	0.0
35	0.0	0.0	0.0	2.579190451043998	0.0
36	0.0	0.0	0.0	2.6571445335747397	0.0
37	0.0	0.0	0.0	2.7443189914585795	0.0
38	0.0	0.0	0.0	2.8365227449895642	0.0
39	0.0	0.0	0.0	2.9664462158741336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTGG	30	2.1491232E-6	45.000004	2
GCGATAG	30	2.1491232E-6	45.000004	9
TGAACGG	20	7.007866E-4	45.0	2
AATGGGC	20	7.007866E-4	45.0	4
GTAGGGT	35	1.1997327E-7	45.0	4
ATAGAAC	20	7.007866E-4	45.0	37
CACGAGC	25	3.8697504E-5	45.0	41
TACGGGA	25	3.8697504E-5	45.0	4
TAACGGG	20	7.007866E-4	45.0	3
CGACAGG	20	7.007866E-4	45.0	2
TATGCCA	25	3.8697504E-5	45.0	10
TCGATCA	25	3.8697504E-5	45.0	17
ACGGGAT	40	6.726623E-9	45.0	5
GTAATCT	20	7.007866E-4	45.0	18
CTACGGG	20	7.007866E-4	45.0	3
AGCATAT	45	3.783498E-10	45.0	31
CAAGGGT	20	7.007866E-4	45.0	4
CCATAGC	20	7.007866E-4	45.0	35
CGGGATC	35	1.1997327E-7	45.0	6
GGAGTGC	25	3.8697504E-5	45.0	8
>>END_MODULE