FastQCFastQC Report
Thu 26 May 2016
SRR1545491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545491_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2468141
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135270.5480643123711328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66780.2705680104985898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT302.1665182E-645.00000442
CGAGTTA351.2126839E-745.013
CGTAAAC253.8921127E-545.035
ATACGAG900.042.51
CGTTTTT90850.041.507981
CGACATA556.184564E-1140.90909214
ATACCGG1000.040.52
AACGGGA4200.040.1785744
AGCGTAA403.4603727E-739.37518
TCGCATA403.4603727E-739.37538
ACCGGTA403.4603727E-739.37541
CGCATAT403.4603727E-739.37539
TACGGGA5300.039.0566024
TATGGGA16900.038.875744
TTAACGG2200.038.863642
ATCGGAT356.2512045E-638.57142633
CCCGAAT356.2512045E-638.57142642
GGGCGAT44400.038.1587837
GGCGATA10150.037.684738
ATTACGT301.1403015E-437.50000420