##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545491_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2468141 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.028637342842245 31.0 31.0 33.0 30.0 34.0 2 31.308854721022826 31.0 31.0 34.0 28.0 34.0 3 31.419931033113585 31.0 31.0 34.0 30.0 34.0 4 35.190788127582664 37.0 35.0 37.0 32.0 37.0 5 35.0419668082172 37.0 35.0 37.0 32.0 37.0 6 35.092283625611344 37.0 35.0 37.0 32.0 37.0 7 35.7137732406698 37.0 35.0 37.0 35.0 37.0 8 35.70515015147028 37.0 35.0 37.0 35.0 37.0 9 37.35820319827757 39.0 37.0 39.0 35.0 39.0 10 36.58589845555825 39.0 35.0 39.0 32.0 39.0 11 36.228428197578666 38.0 35.0 39.0 31.0 39.0 12 35.307707298732126 37.0 35.0 39.0 30.0 39.0 13 34.884893934341676 37.0 34.0 39.0 27.0 39.0 14 35.78984750060876 38.0 34.0 40.0 27.0 41.0 15 36.13473338840852 38.0 34.0 40.0 30.0 41.0 16 36.29552728146407 38.0 34.0 40.0 31.0 41.0 17 36.23363900198571 38.0 34.0 40.0 31.0 41.0 18 36.14912762277358 37.0 35.0 40.0 30.0 41.0 19 36.07049435182188 37.0 34.0 40.0 30.0 41.0 20 35.92889101554571 37.0 34.0 40.0 30.0 41.0 21 35.76898159383925 37.0 34.0 40.0 30.0 41.0 22 35.649988392073226 36.0 34.0 40.0 30.0 41.0 23 35.600062557203984 36.0 34.0 40.0 29.0 41.0 24 35.538863055230635 36.0 34.0 40.0 29.0 41.0 25 35.42243494192593 36.0 34.0 40.0 29.0 41.0 26 35.25185878764625 36.0 34.0 40.0 29.0 41.0 27 35.148592807299096 36.0 34.0 40.0 29.0 41.0 28 35.15208815055542 36.0 34.0 40.0 28.0 41.0 29 35.16741547585814 36.0 34.0 40.0 29.0 41.0 30 35.096678431256564 36.0 34.0 40.0 28.0 41.0 31 34.9243872209894 36.0 34.0 40.0 27.0 41.0 32 34.740337768385196 35.0 33.0 40.0 26.0 41.0 33 34.535163104539 35.0 33.0 40.0 25.0 41.0 34 34.39747283481778 35.0 33.0 40.0 25.0 41.0 35 34.247607814950605 35.0 33.0 40.0 24.0 41.0 36 34.06661977577456 35.0 33.0 40.0 23.0 41.0 37 33.98488781637678 35.0 33.0 40.0 23.0 41.0 38 33.96172058241405 35.0 33.0 40.0 23.0 41.0 39 33.90631288893139 35.0 33.0 40.0 23.0 41.0 40 33.75893476102054 35.0 33.0 40.0 23.0 41.0 41 33.71384698037916 35.0 33.0 40.0 23.0 41.0 42 33.665730199368674 35.0 33.0 39.0 23.0 41.0 43 33.58208951595553 35.0 32.0 39.0 23.0 41.0 44 33.46676142084265 35.0 32.0 39.0 23.0 41.0 45 33.38596619885168 35.0 32.0 39.0 23.0 41.0 46 33.31449621395212 35.0 32.0 39.0 23.0 41.0 47 33.22810852378369 35.0 32.0 39.0 23.0 41.0 48 33.16797824759607 35.0 32.0 39.0 23.0 41.0 49 33.15169473705109 35.0 32.0 39.0 23.0 40.0 50 33.083042662473495 35.0 32.0 39.0 23.0 40.0 51 32.9114852028308 35.0 32.0 38.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 6.0 13 7.0 14 34.0 15 120.0 16 349.0 17 916.0 18 1979.0 19 3854.0 20 6373.0 21 9955.0 22 14480.0 23 20205.0 24 27945.0 25 37443.0 26 47423.0 27 56395.0 28 63609.0 29 72963.0 30 85844.0 31 102597.0 32 124952.0 33 157084.0 34 250668.0 35 341516.0 36 182918.0 37 214674.0 38 277553.0 39 366071.0 40 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.993228911962486 23.952116187851505 28.849283732169273 15.205371168016738 2 30.383231752156785 26.236264459769522 29.573310438909285 13.807193349164413 3 28.64483836215192 25.66547048973296 31.348614199918075 14.341076948197044 4 25.605506330473016 28.54682937482097 30.549348679836363 15.298315614869654 5 23.138629438107465 32.56398236567522 29.966399812652515 14.330988383564797 6 21.114798546760497 41.88289891055657 26.190278432228954 10.812024110453981 7 86.65027646313561 4.491842240779599 7.041129335803749 1.8167519602810374 8 87.1900754454466 3.844593967686611 6.866666045416368 2.0986645414504275 9 82.24688135726443 5.728157345953898 8.885553945256774 3.139407351524893 10 50.45416773190835 24.569098767047752 13.403731796522159 11.573001704521744 11 45.273791084058814 20.897793116357615 19.800489518224445 14.027926281359129 12 40.14766579380999 19.692310933613598 24.475789673280417 15.684233599295988 13 22.71802948048754 36.19756731888494 25.401020444131838 15.683382756495678 14 17.554750721291853 38.246558847326796 28.02027923040053 16.17841120098082 15 16.316571865221636 21.971597246672697 45.66493567425848 16.046895213847183 16 18.777979053870908 17.82896520093463 43.615417433606915 19.77763831158755 17 18.989555296881335 17.771918216990034 29.104090892700214 34.134435593428414 18 22.48976051206151 21.349509610674595 34.303834343337755 21.856895533926142 19 29.524002072815126 22.785934839217045 25.935592820669484 21.75447026729834 20 31.30262817237751 22.473878113122385 25.045732800516664 21.17776091398344 21 24.32008544082368 27.07142744275955 27.918907388192167 20.6895797282246 22 24.56504713466532 23.429617675813496 25.041397553867466 26.963937635653718 23 21.30242964239077 28.617813974161123 25.083291432701778 24.99646495074633 24 21.41684774087056 22.567713919099436 36.816697263243874 19.19874107678613 25 19.949387008278702 23.607281755782996 35.05913965207012 21.38419158386818 26 19.3383198123608 31.400150963822572 27.08495989491686 22.176569328899767 27 19.27924701222499 31.10312579386672 28.142516979378406 21.475110214529884 28 17.61937425779159 27.380202346624444 35.61684684951143 19.383576546072533 29 18.608580303961563 23.812699517572135 35.322860403842405 22.2558597746239 30 20.467631306315155 28.833036686315733 29.71665719259961 20.982674814769496 31 29.640851150724373 25.60510116723477 24.468091571753803 20.285956110287053 32 31.02622581124822 23.998547894954136 26.351128237811373 18.624098055986266 33 28.65687171032773 25.76975950725668 24.99439861823129 20.578970164184298 34 20.908773040113996 26.376288874906255 27.75100774226432 24.96393034271543 35 21.564165094295667 24.48770147248476 31.533773799795068 22.414359633424507 36 30.570741298815584 24.713539461481336 24.836992700173937 19.878726539529143 37 22.283370358500587 31.453308380680035 27.37598864894672 18.887332611872658 38 22.612484456925273 31.913087623438045 23.143329331671083 22.3310985879656 39 22.269999971638573 29.814990310521157 25.649790672412966 22.265219045427305 40 25.190781239807613 24.657991581518235 25.06979949686829 25.081427681805863 41 18.81286360868362 24.38292625907515 26.67420540398624 30.13000472825499 42 22.662116953610028 24.809644181592542 24.331186913551537 28.1970519512459 43 22.83560785222562 24.444713652907186 26.501403282875653 26.21827521199154 44 20.49570911872539 27.901606917919196 28.468875967783042 23.133807995572376 45 18.994133641473482 33.98371486880207 24.238688146260685 22.783463343463765 46 22.774306654279474 30.29506823151514 26.1094483661995 20.821176748005886 47 21.908148683563862 26.7216905355083 26.426853247038967 24.943307533888866 48 23.07003530187295 23.810349570790322 30.544041041415383 22.575574085921346 49 22.721635433307902 22.827220973193995 31.853569143740167 22.597574449757936 50 21.217061748093 30.58508407744938 26.170992662088594 22.026861512369024 51 19.594220913635 31.26831084609834 25.86886243533088 23.26860580493578 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2417.0 1 3381.0 2 4345.0 3 13875.0 4 23405.0 5 16834.5 6 10264.0 7 10680.5 8 11097.0 9 11830.5 10 12564.0 11 12754.5 12 12945.0 13 12712.5 14 12480.0 15 11962.0 16 11444.0 17 10728.5 18 10013.0 19 9804.0 20 9595.0 21 9202.0 22 8809.0 23 9714.5 24 10620.0 25 10758.5 26 13204.0 27 15511.0 28 18192.5 29 20874.0 30 23879.5 31 26885.0 32 32124.5 33 37364.0 34 41263.0 35 45162.0 36 51337.5 37 57513.0 38 69789.0 39 82065.0 40 116563.0 41 151061.0 42 177350.0 43 203639.0 44 216100.0 45 228561.0 46 219922.0 47 211283.0 48 195753.5 49 180224.0 50 176097.5 51 171971.0 52 162885.0 53 153799.0 54 143290.5 55 132782.0 56 131446.5 57 130111.0 58 122244.0 59 114377.0 60 107635.5 61 100894.0 62 88655.5 63 76417.0 64 65831.0 65 55245.0 66 47341.5 67 39438.0 68 34381.0 69 29324.0 70 23801.0 71 18278.0 72 16149.5 73 14021.0 74 11419.0 75 7114.5 76 5412.0 77 4258.0 78 3104.0 79 2402.0 80 1700.0 81 1218.5 82 737.0 83 542.5 84 348.0 85 297.5 86 247.0 87 144.0 88 41.0 89 32.0 90 23.0 91 17.5 92 12.0 93 8.5 94 5.0 95 3.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2468141.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.04737773148299 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84359967011277 13.78171380749206 2 7.038813234343213 2.399866159744205 3 2.443046328837048 1.249426007495939 4 1.2999141758742312 0.8864051189854973 5 0.8298960322783471 0.7073775570053993 6 0.5641532802235321 0.5770400439855436 7 0.41372827001925794 0.4937087468047896 8 0.34243128759022434 0.4670044405302911 9 0.24293699090935866 0.37272947840835186 >10 2.9010620970426495 12.224948334701219 >50 1.3920674467463017 17.27296458091557 >100 1.6792996418736494 47.72165167441697 >500 0.007860551498206898 0.8357651071104717 >1k 7.145955907460816E-4 0.18894252470046763 >5k 2.3819853024869387E-4 0.27117089618519724 >10k+ 2.3819853024869387E-4 0.5492855215179938 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13527 0.5480643123711328 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 6678 0.2705680104985898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.103264764857437E-5 0.0 0.0 0.030427759192039678 0.0 2 8.103264764857437E-5 0.0 0.0 0.1154310065753942 0.0 3 8.103264764857437E-5 0.0 0.0 0.16522556855544315 0.0 4 8.103264764857437E-5 0.0 0.0 0.25942602144691085 0.0 5 8.103264764857437E-5 0.0 0.0 0.4413443154179603 0.0 6 8.103264764857437E-5 0.0 0.0 0.7148700175557231 0.0 7 8.103264764857437E-5 0.0 0.0 0.8542866878350953 0.0 8 8.103264764857437E-5 0.0 0.0 1.2234714305220002 0.0 9 8.103264764857437E-5 0.0 0.0 1.3788515323881416 0.0 10 8.103264764857437E-5 0.0 0.0 1.6403844026739154 0.0 11 8.103264764857437E-5 0.0 0.0 1.8385092261746796 0.0 12 8.103264764857437E-5 0.0 0.0 2.0170646652683133 0.0 13 8.103264764857437E-5 0.0 0.0 2.100285194403399 0.0 14 8.103264764857437E-5 0.0 0.0 2.133265481996369 0.0 15 8.103264764857437E-5 0.0 0.0 2.179535123803705 0.0 16 8.103264764857437E-5 0.0 0.0 2.2738571256666456 0.0 17 8.103264764857437E-5 0.0 0.0 2.3778625289235906 0.0 18 8.103264764857437E-5 0.0 0.0 2.537780459058052 0.0 19 8.103264764857437E-5 0.0 0.0 2.600094565099806 0.0 20 1.2154897147286156E-4 0.0 0.0 2.6772376456612488 0.0 21 1.2154897147286156E-4 0.0 0.0 2.766049427484086 0.0 22 1.2154897147286156E-4 0.0 0.0 2.8617895006808767 0.0 23 1.2154897147286156E-4 0.0 0.0 2.9709404770635066 0.0 24 1.2154897147286156E-4 0.0 0.0 3.056510952980401 0.0 25 1.2154897147286156E-4 0.0 0.0 3.1317902826459267 0.0 26 1.2154897147286156E-4 0.0 0.0 3.204517083910522 0.0 27 1.2154897147286156E-4 0.0 0.0 3.2749749710409577 0.0 28 1.2154897147286156E-4 0.0 0.0 3.349403457906173 0.0 29 1.2154897147286156E-4 0.0 0.0 3.425979309934076 0.0 30 1.6206529529714874E-4 0.0 0.0 3.5414913491571185 0.0 31 1.6206529529714874E-4 0.0 0.0 3.636947808087139 0.0 32 1.6206529529714874E-4 0.0 0.0 3.723976871661708 0.0 33 1.6206529529714874E-4 0.0 0.0 3.8163135736572587 0.0 34 1.6206529529714874E-4 0.0 0.0 3.9104329939010776 0.0 35 1.6206529529714874E-4 0.0 0.0 4.033845716269856 0.0 36 1.6206529529714874E-4 0.0 0.0 4.133799487144373 0.0 37 1.6206529529714874E-4 0.0 0.0 4.234563584495375 0.0 38 1.6206529529714874E-4 0.0 0.0 4.340594803943535 0.0 39 1.6206529529714874E-4 0.0 0.0 4.4439924623431155 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTAT 30 2.1665182E-6 45.000004 42 CGAGTTA 35 1.2126839E-7 45.0 13 CGTAAAC 25 3.8921127E-5 45.0 35 ATACGAG 90 0.0 42.5 1 CGTTTTT 9085 0.0 41.50798 1 CGACATA 55 6.184564E-11 40.909092 14 ATACCGG 100 0.0 40.5 2 AACGGGA 420 0.0 40.178574 4 AGCGTAA 40 3.4603727E-7 39.375 18 TCGCATA 40 3.4603727E-7 39.375 38 ACCGGTA 40 3.4603727E-7 39.375 41 CGCATAT 40 3.4603727E-7 39.375 39 TACGGGA 530 0.0 39.056602 4 TATGGGA 1690 0.0 38.87574 4 TTAACGG 220 0.0 38.86364 2 ATCGGAT 35 6.2512045E-6 38.571426 33 CCCGAAT 35 6.2512045E-6 38.571426 42 GGGCGAT 4440 0.0 38.158783 7 GGCGATA 1015 0.0 37.68473 8 ATTACGT 30 1.1403015E-4 37.500004 20 >>END_MODULE