FastQCFastQC Report
Thu 26 May 2016
SRR1545490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545490_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1838121
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105200.572323584791208No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23030.1252909900926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA253.8917213E-545.026
GTACGTC207.034392E-445.029
CGTTTTT85700.041.8494721
CGTTCGA501.0822987E-940.514
ACATACG1300.039.8076917
TAGGGCG8950.038.7150845
ACCCGCT4950.038.63636434
TACGGGA5550.038.513514
GGGCGAT39850.038.2810557
GCGATCG1000.038.259
GCGATAT2250.038.09
AATGCGT301.14018825E-437.49999630
AGGGCGA20500.037.426836
CGGGTAC1450.037.241386
CGGTCTA1450.037.2413831
ATTGGGC6500.037.0384644
AGTACGG2800.036.9642832
TCGGGTA552.752131E-936.8181845
GGCGATA8100.036.388898
ATGGGCG6200.036.2903255
Produced by FastQC (version 0.11.5)