##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545490_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1838121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151689687458006	31.0	31.0	33.0	30.0	34.0
2	31.4320798249952	31.0	31.0	34.0	30.0	34.0
3	31.509949562624005	31.0	31.0	34.0	30.0	34.0
4	35.30851287809671	37.0	35.0	37.0	33.0	37.0
5	35.197562075619615	37.0	35.0	37.0	32.0	37.0
6	35.24328648658059	37.0	35.0	37.0	32.0	37.0
7	35.79369040449459	37.0	35.0	37.0	35.0	37.0
8	35.778378572466124	37.0	35.0	37.0	35.0	37.0
9	37.421411865704165	39.0	37.0	39.0	35.0	39.0
10	36.71943305146941	39.0	37.0	39.0	32.0	39.0
11	36.44038504538058	38.0	35.0	39.0	32.0	39.0
12	35.76826008733919	37.0	35.0	39.0	31.0	39.0
13	35.50006283590689	37.0	35.0	39.0	30.0	39.0
14	36.47885802947684	38.0	35.0	40.0	31.0	41.0
15	36.666873943554315	38.0	35.0	40.0	31.0	41.0
16	36.75054961017256	38.0	35.0	40.0	31.0	41.0
17	36.627440739755436	38.0	35.0	40.0	31.0	41.0
18	36.53270540949154	38.0	35.0	40.0	31.0	41.0
19	36.47871222841152	38.0	35.0	40.0	31.0	41.0
20	36.36517019282191	38.0	35.0	40.0	31.0	41.0
21	36.237619830250566	38.0	34.0	40.0	30.0	41.0
22	36.133720794224104	38.0	34.0	40.0	30.0	41.0
23	36.06697056396178	38.0	34.0	40.0	30.0	41.0
24	35.96576231923796	37.0	34.0	40.0	30.0	41.0
25	35.85640227166764	37.0	34.0	40.0	30.0	41.0
26	35.685161096576344	37.0	34.0	40.0	30.0	41.0
27	35.58272605557523	37.0	34.0	40.0	29.0	41.0
28	35.55940114932586	37.0	34.0	40.0	29.0	41.0
29	35.52966643654036	37.0	34.0	40.0	29.0	41.0
30	35.42617107361267	37.0	34.0	40.0	29.0	41.0
31	35.284399666833686	37.0	34.0	40.0	28.0	41.0
32	35.11392884363978	37.0	34.0	40.0	27.0	41.0
33	34.891794392208126	37.0	33.0	40.0	26.0	41.0
34	34.684908120847325	37.0	33.0	40.0	25.0	41.0
35	34.53725842858005	36.0	33.0	40.0	24.0	41.0
36	34.3973884200224	36.0	33.0	40.0	24.0	41.0
37	34.3525747216859	36.0	33.0	40.0	24.0	41.0
38	34.271265058176255	36.0	33.0	40.0	24.0	41.0
39	34.21397394404394	36.0	33.0	40.0	23.0	41.0
40	34.06677688791979	36.0	33.0	40.0	23.0	41.0
41	33.993864386512094	36.0	33.0	40.0	23.0	41.0
42	33.93495313964641	35.0	33.0	40.0	23.0	41.0
43	33.846812587419436	35.0	33.0	40.0	23.0	41.0
44	33.69514194114533	35.0	33.0	40.0	23.0	41.0
45	33.579029889762424	35.0	32.0	40.0	23.0	41.0
46	33.526158506431294	35.0	32.0	39.0	23.0	41.0
47	33.44292894755024	35.0	32.0	39.0	23.0	41.0
48	33.361810783947305	35.0	32.0	39.0	23.0	41.0
49	33.354250889903334	35.0	32.0	39.0	23.0	41.0
50	33.30110204932102	35.0	32.0	39.0	23.0	41.0
51	33.14303954962704	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	13.0
14	26.0
15	65.0
16	204.0
17	533.0
18	1186.0
19	2331.0
20	4097.0
21	6416.0
22	9645.0
23	13748.0
24	19187.0
25	26231.0
26	33514.0
27	38712.0
28	43377.0
29	49379.0
30	58395.0
31	71186.0
32	86856.0
33	110814.0
34	171958.0
35	222516.0
36	149808.0
37	179508.0
38	233736.0
39	304531.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.66775092608158	23.383226675501774	26.93870534094328	16.010317057473365
2	33.53402741168835	25.707447986286	27.6761431918791	13.082381410146557
3	29.79771190253525	25.563007005523573	30.945786485220506	13.69349460672067
4	26.355065852574448	28.430881318476857	30.258399746262622	14.955653082686068
5	22.65449336577951	32.06208949247629	29.954774468057327	15.328642673686879
6	21.577360793984727	40.698517671034715	27.49470791095907	10.229413624021486
7	85.53299809968985	4.305483697754391	8.09603937934445	2.065478823211312
8	86.04090807950075	3.706720069027012	7.958725241700628	2.293646609771609
9	80.40139903738654	6.106398871456232	9.763611862331151	3.7285902288260675
10	45.070754319220555	27.015359707005143	15.188064333087976	12.72582164068633
11	40.820870878467744	23.70480506996003	20.919950318831024	14.554373732741208
12	36.1987594940703	21.158073924404324	25.928271316197353	16.714895265328018
13	24.001412311811897	30.914613346999463	27.020147204672597	18.06382713651604
14	19.337573532971987	34.06647331704496	29.409761381323644	17.18619176865941
15	19.31211274992234	22.59954594936895	41.118457381206134	16.96988391950258
16	21.76809905332674	19.77731607440424	40.18979164048504	18.26479323178398
17	21.03724401168367	19.646747956200926	30.382493861938357	28.933514170177045
18	24.13720315474335	22.668039808043105	31.5911194094404	21.603637627773146
19	29.272719260592744	24.028722809869425	26.674250498198976	20.02430743133885
20	31.602870540078698	23.497038551869	25.291642933191017	19.608447974861285
21	26.494828142434585	25.8690260325626	27.581263692651355	20.054882132351462
22	25.237892391197313	23.913442042172413	26.36431442761385	24.484351139016418
23	23.870953000373753	27.464405226859384	25.872072621987346	22.792569150779517
24	23.019213642627445	22.835711033169197	33.42152121650316	20.723554107700203
25	22.071833138297205	22.951481431309475	32.384211920760386	22.592473509632935
26	20.40121406588576	29.264613156587625	27.904637398734906	22.429535378791712
27	21.022663905150967	29.237411465295267	27.975144182564694	21.76478044698907
28	19.850271010450346	27.623371910771926	32.91235995889281	19.613997119884928
29	21.875545733931553	24.76735753522211	32.544103462176864	20.812993268669473
30	22.91269181952657	26.40712988970802	29.963587816036053	20.716590474729358
31	28.558457250638014	25.19377124792111	26.005524119467655	20.242247381973222
32	29.77094543830357	24.907011018317075	27.087825012607986	18.234218530771372
33	28.290520591408292	25.714139602343916	25.583081853697337	20.41225795255046
34	23.011597169065585	26.95965064323839	28.35085394269474	21.67789824500128
35	22.79349400828346	26.181410255364035	29.03742463091385	21.987671105438654
36	28.05190735539173	27.105560515330602	25.30453653486359	19.537995594414078
37	24.97637533111259	30.166566836459623	25.66441491066149	19.192642921766303
38	24.20689388783437	30.22771623848484	23.680758774857587	21.884631098823203
39	23.917522295866267	28.4720102757109	25.40676049074027	22.203706937682558
40	25.267759848236327	24.7951576637229	26.40789153706421	23.529190950976567
41	20.05069307189244	25.904279424477494	26.941806333750606	27.10322116987946
42	23.767749783610544	25.783014284696165	24.473144042203966	25.97609188948932
43	23.774495803051053	25.39016745905193	25.493588289345475	25.341748448551538
44	23.343512206214932	27.875966816112758	26.810639778338857	21.96988119933345
45	21.752974912968188	30.430260031847737	24.955593238965225	22.861171816218846
46	22.819390018393783	29.742710082742104	25.814840263508227	21.623059635355887
47	23.287585528917845	26.959759450003563	26.763200028724988	22.989454992353604
48	23.88221450056879	24.575259191315478	28.929216302952852	22.61331000516288
49	23.098098547375283	24.420590374627132	29.425755975803554	23.055555102194035
50	20.953190785590284	29.359003025372104	26.712006445712767	22.97579974332484
51	20.573835998827064	30.145784744312266	25.810814413196955	23.469564843663722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1986.0
1	2887.0
2	3788.0
3	12254.0
4	20720.0
5	15093.5
6	9467.0
7	9449.0
8	9431.0
9	9991.0
10	10551.0
11	10660.0
12	10769.0
13	10535.5
14	10302.0
15	10267.5
16	10233.0
17	9620.5
18	9008.0
19	8612.5
20	8217.0
21	8410.5
22	8604.0
23	9405.5
24	10207.0
25	11033.5
26	12872.5
27	13885.0
28	16821.5
29	19758.0
30	21189.5
31	22621.0
32	25727.0
33	28833.0
34	32713.0
35	36593.0
36	40286.0
37	43979.0
38	50801.0
39	57623.0
40	76332.0
41	95041.0
42	111086.5
43	127132.0
44	131935.0
45	136738.0
46	131600.0
47	126462.0
48	124258.5
49	122055.0
50	123197.5
51	124340.0
52	120565.0
53	116790.0
54	113168.5
55	109547.0
56	104414.0
57	99281.0
58	100278.5
59	101276.0
60	95185.0
61	89094.0
62	79816.0
63	70538.0
64	61721.0
65	52904.0
66	44198.5
67	35493.0
68	29099.5
69	22706.0
70	19599.0
71	16492.0
72	14116.0
73	11740.0
74	10850.5
75	7814.5
76	5668.0
77	4314.5
78	2961.0
79	2415.5
80	1870.0
81	1458.5
82	1047.0
83	678.5
84	310.0
85	235.5
86	161.0
87	101.5
88	42.0
89	30.5
90	19.0
91	12.0
92	5.0
93	4.0
94	3.0
95	4.5
96	6.0
97	5.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1838121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.214693447812596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30578115208553	15.238362734766373
2	5.904532307304551	2.269075564751251
3	2.2012665872899544	1.2688998801506721
4	1.2385210553042227	0.9519120962532795
5	0.844946218412991	0.8117691283347064
6	0.6186224563273826	0.7131984514958094
7	0.49263058446137553	0.6626021964409456
8	0.40020349632971786	0.6151829998974657
9	0.3448396784030322	0.5963389838240891
>10	5.71026195128712	27.69967115198075
>50	2.239589971760362	30.603037354611
>100	0.6962531248603703	17.571752508345632
>500	0.001417453429697453	0.1683725385984529
>1k	8.504720578184718E-4	0.25678037753254834
>5k	0.0	0.0
>10k+	2.834906859394906E-4	0.5730440330170574
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10520	0.572323584791208	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2303	0.1252909900926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01664743507092297	0.0
2	0.0	0.0	0.0	0.05500181979314746	0.0
3	0.0	0.0	0.0	0.08590294110126591	0.0
4	0.0	0.0	0.0	0.1292080336386995	0.0
5	0.0	0.0	0.0	0.2041758948404376	0.0
6	0.0	0.0	0.0	0.37016061510640486	0.0
7	0.0	0.0	0.0	0.46101426402287987	0.0
8	0.0	0.0	0.0	0.7039797706462197	0.0
9	0.0	0.0	0.0	0.8652313966273167	0.0
10	0.0	0.0	0.0	1.1248987417041643	0.0
11	0.0	0.0	0.0	1.2953445393420782	0.0
12	0.0	0.0	0.0	1.4448450346848765	0.0
13	0.0	0.0	0.0	1.522424258250681	0.0
14	0.0	0.0	0.0	1.5560455486880351	0.0
15	0.0	0.0	0.0	1.5932030589933959	0.0
16	0.0	0.0	0.0	1.6605000432506891	0.0
17	0.0	0.0	0.0	1.7294291289855237	0.0
18	0.0	0.0	0.0	1.8401944159280048	0.0
19	0.0	0.0	0.0	1.887198938481199	0.0
20	0.0	0.0	0.0	1.9442680868125657	0.0
21	0.0	0.0	0.0	2.0074304139934203	0.0
22	0.0	0.0	0.0	2.0742921711900357	0.0
23	0.0	0.0	0.0	2.1477911410619868	0.0
24	0.0	0.0	0.0	2.2094301735304693	0.0
25	0.0	0.0	0.0	2.2637247493500157	0.0
26	0.0	0.0	0.0	2.323350856662864	0.0
27	0.0	0.0	0.0	2.3802023914638917	0.0
28	0.0	0.0	0.0	2.4359658586132253	0.0
29	0.0	0.0	0.0	2.4904236445805252	0.0
30	0.0	0.0	0.0	2.572572752283446	0.0
31	0.0	0.0	0.0	2.641393031253111	0.0
32	0.0	0.0	0.0	2.714130353768876	0.0
33	0.0	0.0	0.0	2.779740833166043	0.0
34	0.0	0.0	0.0	2.8517709117082064	0.0
35	0.0	0.0	0.0	2.929513345421765	0.0
36	0.0	0.0	0.0	3.0129681343067185	0.0
37	0.0	0.0	0.0	3.0956068724529016	0.0
38	0.0	0.0	0.0	3.188854270203104	0.0
39	0.0	0.0	0.0	3.277694993963945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACGA	25	3.8917213E-5	45.0	26
GTACGTC	20	7.034392E-4	45.0	29
CGTTTTT	8570	0.0	41.849472	1
CGTTCGA	50	1.0822987E-9	40.5	14
ACATACG	130	0.0	39.80769	17
TAGGGCG	895	0.0	38.715084	5
ACCCGCT	495	0.0	38.636364	34
TACGGGA	555	0.0	38.51351	4
GGGCGAT	3985	0.0	38.281055	7
GCGATCG	100	0.0	38.25	9
GCGATAT	225	0.0	38.0	9
AATGCGT	30	1.14018825E-4	37.499996	30
AGGGCGA	2050	0.0	37.42683	6
CGGGTAC	145	0.0	37.24138	6
CGGTCTA	145	0.0	37.24138	31
ATTGGGC	650	0.0	37.038464	4
AGTACGG	280	0.0	36.964283	2
TCGGGTA	55	2.752131E-9	36.818184	5
GGCGATA	810	0.0	36.38889	8
ATGGGCG	620	0.0	36.290325	5
>>END_MODULE