FastQCFastQC Report
Thu 26 May 2016
SRR1545489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545489_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2930305
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290870.9926270473551388No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31030.10589341382552328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT211850.043.0033041
TAGGGCG13450.039.3122675
TATAGCG1450.038.7931061
AGGGCGA30350.038.4761126
GGGCGAT60350.038.140027
CGTAAGG4550.038.0769232
GCGCGAC12600.037.321439
CGCGACC12850.037.12062510
GTTTTTT257050.036.859562
CTATGCG1100.036.818181
CGACCCG12600.036.78571332
TGCGTAG1900.036.7105261
ACGGGAT10250.036.6585355
ATAGGGC15900.036.6509444
ACCCGCT12650.036.6403234
GGCGATA14050.036.5124558
TGGGCGA18550.036.3881386
TACGCGG1800.036.252
CGACGGT2250.036.028
ACGTATA250.002107640336.010
Produced by FastQC (version 0.11.5)