##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545489_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2930305
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02930923572802	31.0	31.0	33.0	30.0	34.0
2	31.338411189278933	31.0	31.0	34.0	30.0	34.0
3	31.38934377138216	31.0	31.0	34.0	28.0	34.0
4	35.20170425945422	37.0	35.0	37.0	32.0	37.0
5	35.08186724590102	37.0	35.0	37.0	32.0	37.0
6	35.127013740890455	37.0	35.0	37.0	32.0	37.0
7	35.73057104977127	37.0	35.0	37.0	35.0	37.0
8	35.71862246421448	37.0	35.0	37.0	35.0	37.0
9	37.3735102659962	39.0	37.0	39.0	35.0	39.0
10	36.595713415497706	39.0	35.0	39.0	32.0	39.0
11	36.31220470224089	38.0	35.0	39.0	31.0	39.0
12	35.90833309160651	37.0	35.0	39.0	31.0	39.0
13	35.75739317238308	37.0	35.0	39.0	30.0	39.0
14	36.75490025782299	39.0	35.0	41.0	31.0	41.0
15	36.907774787948696	39.0	35.0	41.0	31.0	41.0
16	36.9497775828796	38.0	35.0	41.0	31.0	41.0
17	36.858571036120814	38.0	35.0	41.0	31.0	41.0
18	36.70382298088424	38.0	35.0	40.0	31.0	41.0
19	36.5999549534946	38.0	35.0	40.0	31.0	41.0
20	36.44454997005431	38.0	35.0	40.0	31.0	41.0
21	36.32597289360664	38.0	35.0	40.0	30.0	41.0
22	36.25092575687514	38.0	35.0	40.0	30.0	41.0
23	36.15182139743133	38.0	34.0	40.0	30.0	41.0
24	36.03919694366286	38.0	34.0	40.0	30.0	41.0
25	35.91653872207842	38.0	34.0	40.0	30.0	41.0
26	35.77320040064089	38.0	34.0	40.0	29.0	41.0
27	35.67497854318919	37.0	34.0	40.0	29.0	41.0
28	35.613480166740324	37.0	34.0	40.0	29.0	41.0
29	35.55969498055663	37.0	34.0	40.0	29.0	41.0
30	35.43433226234129	37.0	34.0	40.0	27.0	41.0
31	35.262140971673595	37.0	34.0	40.0	27.0	41.0
32	35.03907238325021	37.0	34.0	40.0	26.0	41.0
33	34.76542748962992	37.0	33.0	40.0	24.0	41.0
34	34.492909441167384	37.0	33.0	40.0	23.0	41.0
35	34.27653844906929	37.0	33.0	40.0	23.0	41.0
36	34.11835423275051	37.0	33.0	40.0	22.0	41.0
37	34.06828333569373	37.0	33.0	40.0	22.0	41.0
38	33.97118456952433	37.0	33.0	40.0	22.0	41.0
39	33.87662649451166	37.0	33.0	40.0	21.0	41.0
40	33.765913104608565	37.0	33.0	40.0	21.0	41.0
41	33.645847445914335	36.0	33.0	40.0	20.0	41.0
42	33.57432656327584	36.0	32.0	40.0	20.0	41.0
43	33.509653773242036	36.0	32.0	40.0	20.0	41.0
44	33.372935581790976	36.0	32.0	40.0	19.0	41.0
45	33.29628758781082	36.0	32.0	40.0	20.0	41.0
46	33.23784554850092	36.0	32.0	40.0	20.0	41.0
47	33.129619271714034	35.0	32.0	40.0	20.0	41.0
48	33.028133931450824	35.0	31.0	39.0	19.0	41.0
49	32.97742589935177	35.0	31.0	39.0	19.0	41.0
50	32.91664894951208	35.0	31.0	39.0	18.0	41.0
51	32.76164631326773	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	2.0
13	9.0
14	43.0
15	115.0
16	465.0
17	1161.0
18	2614.0
19	4939.0
20	8307.0
21	12589.0
22	18306.0
23	25950.0
24	36294.0
25	49542.0
26	63655.0
27	72613.0
28	76445.0
29	83111.0
30	94024.0
31	110943.0
32	131864.0
33	162590.0
34	232227.0
35	295915.0
36	242721.0
37	292814.0
38	387370.0
39	523487.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.263571539481383	23.557001745552082	26.599927311320837	18.5794994036457
2	35.13272509175666	24.994804295116037	26.647396772690897	13.225073840436405
3	27.831403215706214	25.203997536092658	33.274932131638174	13.689667116562951
4	24.7785128169252	27.433151156620216	32.878215748872556	14.910120277582026
5	22.126229180921438	31.112187980432072	32.265242014056554	14.496340824589932
6	20.599391530915725	39.696106719266425	29.48256239538205	10.2219393544358
7	84.20836738837765	3.8246189389841674	10.219789407587266	1.7472242650509076
8	84.64091621861888	3.123463257237728	10.294593907460145	1.9410266166832464
9	79.19250726460216	5.501202093297455	11.997658946764927	3.3086316953354684
10	38.03720090570777	29.392230501603073	18.6262863422067	13.944282250482459
11	31.402362552703554	24.147793489073663	28.00025935866744	16.449584599555337
12	28.68970977423852	22.054461907548873	31.133823953479244	18.122004364733364
13	22.707499731256643	27.5466888259072	31.99663516255134	17.749176280284818
14	19.36197767809153	30.31090620259666	32.671343085446736	17.655773033865074
15	18.37907658076548	23.713913739354776	39.575129551360696	18.331880128519046
16	20.997882472984895	22.015182719887523	38.09705815606225	18.889876651065332
17	20.952938346008352	21.79513736624686	32.200880113162285	25.05104417458251
18	22.692620734019155	22.90945823045724	34.091297663553796	20.306623371969813
19	26.699882776707547	24.100562910686772	29.30524296958849	19.894311343017197
20	27.45267130895931	24.070088267262282	29.227606000058014	19.249634423720398
21	24.271569000496534	24.95317040376343	31.806723190930636	18.968537404809396
22	23.73299025186798	23.252152932885824	30.100689177406448	22.914167637839746
23	21.261404529562622	26.418888136217898	30.417755148354864	21.901952185864612
24	21.095449108539896	24.296208073903568	35.122282492778055	19.486060324778478
25	21.42602903110768	25.1196718430334	33.209068680563966	20.245230445294943
26	20.081970989368	28.38752280052759	30.131368577673655	21.399137632430755
27	20.280926388208737	27.08871602102853	31.509382129164027	21.120975461598707
28	19.275263155200566	26.361897481661465	34.69045031148635	19.67238905165162
29	20.544209561803292	24.162706612451604	34.37157565509392	20.92150817065118
30	20.950140002491207	26.19839914275135	32.693149689196176	20.158311165561262
31	24.660368118677066	25.6286973540297	29.727895219098354	19.98303930819488
32	25.65463322077395	25.350091543371768	29.500478619119853	19.494796616734437
33	24.921364840861276	25.939176979870698	28.646437828144165	20.49302035112386
34	21.358868786696267	26.854781328223513	30.09437584142265	21.691974043657574
35	21.994161017368498	26.173828321625226	30.81781589288487	21.014194768121406
36	25.46936240425485	26.30528221465001	28.01745893345573	20.20789644763941
37	22.417222780563797	28.73673559578269	28.73547292858593	20.11056869506758
38	22.386406875734778	28.84948836383926	27.665140659419414	21.09896410100655
39	22.14387239553562	28.19672354925511	28.002682314639603	21.65672174056967
40	24.06234163337946	26.019305157654237	28.529897058497323	21.38845615046898
41	20.83124452915311	25.46956716109757	29.142666036470604	24.55652227327872
42	22.254270459900933	26.90324044766671	27.39568065440287	23.446808438029485
43	22.0281847794001	25.97719350033529	28.785843111894494	23.208778608370118
44	21.82666992002539	27.39496400545336	29.470925381487596	21.307440693033662
45	20.911679842200726	29.254292641892228	27.912725808405607	21.921301707501435
46	22.1858134221523	28.747963095991715	27.994184905666813	21.07203857618917
47	21.573488084004907	27.695274041439372	28.73421026138917	21.997027613166548
48	22.269149457138422	26.26914263191033	29.87190753180983	21.589800379141423
49	22.162948908048822	26.017496472210233	30.01646586276855	21.803088756972397
50	20.893388230917942	28.869179146880615	28.942823357978092	21.29460926422335
51	20.841687128131714	29.30060181448689	28.17382490901118	21.68388614837022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3015.0
1	4040.5
2	5066.0
3	27652.5
4	50239.0
5	36150.0
6	22061.0
7	22329.5
8	22598.0
9	23471.5
10	24345.0
11	24220.0
12	24095.0
13	23419.5
14	22744.0
15	21654.5
16	20565.0
17	20121.5
18	19678.0
19	18895.5
20	18113.0
21	17706.5
22	17300.0
23	18404.5
24	19509.0
25	19405.0
26	22698.5
27	26096.0
28	32870.0
29	39644.0
30	45040.0
31	50436.0
32	58053.0
33	65670.0
34	72936.5
35	80203.0
36	88288.0
37	96373.0
38	104731.0
39	113089.0
40	133655.5
41	154222.0
42	175892.0
43	197562.0
44	202452.5
45	207343.0
46	212893.5
47	218444.0
48	212091.0
49	205738.0
50	200102.5
51	194467.0
52	184222.0
53	173977.0
54	164200.5
55	154424.0
56	147363.5
57	140303.0
58	130916.0
59	121529.0
60	114683.0
61	107837.0
62	95443.0
63	83049.0
64	73380.5
65	63712.0
66	54547.0
67	45382.0
68	38826.5
69	32271.0
70	27961.5
71	23652.0
72	21387.0
73	19122.0
74	15767.0
75	9756.5
76	7101.0
77	5489.5
78	3878.0
79	2993.0
80	2108.0
81	1550.5
82	993.0
83	728.0
84	463.0
85	273.5
86	84.0
87	68.0
88	52.0
89	39.5
90	27.0
91	16.5
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2930305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.470028904679513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77003392465903	16.124248712603563
2	5.858040267127686	2.3982850718576048
3	2.4176428146680045	1.484676548923344
4	1.475668188032265	1.2082788185094604
5	0.9816973974340597	1.0047687050561926
6	0.7492862429890642	0.9202746631118917
7	0.5896767408864158	0.84494899512535
8	0.4529044759868336	0.7416774171607371
9	0.4006379586889675	0.7380963535207465
>10	5.630095836595997	28.682018997169294
>50	2.20332201047892	31.81968356379607
>100	0.46645921905762827	12.258304212400182
>500	0.0036951227664897635	0.522289158220237
>1k	6.718405029981388E-4	0.25239449084950033
>5k	0.0	0.0
>10k+	1.679601257495347E-4	1.0000542916958748
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29087	0.9926270473551388	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3103	0.10589341382552328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4126140452956264E-5	0.0	0.0	0.011534635473099217	0.0
2	3.4126140452956264E-5	0.0	0.0	0.05944773666904981	0.0
3	3.4126140452956264E-5	0.0	0.0	0.1272563777490739	0.0
4	3.4126140452956264E-5	0.0	0.0	0.19588404619996894	0.0
5	3.4126140452956264E-5	0.0	0.0	0.3179191244597405	0.0
6	3.4126140452956264E-5	0.0	0.0	0.6091174809448163	0.0
7	6.825228090591253E-5	0.0	0.0	0.766200105449774	0.0
8	6.825228090591253E-5	0.0	0.0	1.0964387666130317	0.0
9	6.825228090591253E-5	0.0	0.0	1.2977829952854738	0.0
10	6.825228090591253E-5	0.0	0.0	1.558950348171948	0.0
11	6.825228090591253E-5	0.0	0.0	1.7182511718063478	0.0
12	6.825228090591253E-5	0.0	0.0	1.8504899660615532	0.0
13	6.825228090591253E-5	0.0	0.0	1.930311008581018	0.0
14	6.825228090591253E-5	0.0	0.0	1.975528144681185	0.0
15	6.825228090591253E-5	0.0	0.0	2.011497096718601	0.0
16	6.825228090591253E-5	0.0	0.0	2.068999643381832	0.0
17	6.825228090591253E-5	0.0	0.0	2.130085434792624	0.0
18	6.825228090591253E-5	0.0	0.0	2.232941622117834	0.0
19	1.0237842135886879E-4	0.0	0.0	2.2756675499649353	0.0
20	1.0237842135886879E-4	0.0	0.0	2.3304741315323834	0.0
21	1.0237842135886879E-4	0.0	0.0	2.3857243529257195	0.0
22	1.0237842135886879E-4	0.0	0.0	2.4462641260892637	0.0
23	1.0237842135886879E-4	0.0	0.0	2.515540191208765	2.730091236236501E-4
24	1.0237842135886879E-4	0.0	0.0	2.574646666473285	2.730091236236501E-4
25	1.0237842135886879E-4	0.0	0.0	2.6237883087255423	2.730091236236501E-4
26	1.0237842135886879E-4	0.0	0.0	2.6751481501072414	2.730091236236501E-4
27	1.0237842135886879E-4	0.0	0.0	2.7358926801135035	2.730091236236501E-4
28	1.0237842135886879E-4	0.0	0.0	2.7896072251864568	2.730091236236501E-4
29	1.0237842135886879E-4	0.0	0.0	2.851034278001778	2.730091236236501E-4
30	1.3650456181182505E-4	0.0	0.0	2.9402741352862587	2.730091236236501E-4
31	1.3650456181182505E-4	0.0	0.0	3.0061034602200114	2.730091236236501E-4
32	1.3650456181182505E-4	0.0	0.0	3.072512929541464	2.730091236236501E-4
33	1.706307022647813E-4	0.0	0.0	3.1426080220318364	2.730091236236501E-4
34	1.706307022647813E-4	0.0	0.0	3.2151601966348213	2.730091236236501E-4
35	1.706307022647813E-4	0.0	0.0	3.2966534200364808	2.730091236236501E-4
36	2.0475684271773758E-4	0.0	0.0	3.372959470089291	2.730091236236501E-4
37	2.0475684271773758E-4	0.0	0.0	3.4504599350579546	2.730091236236501E-4
38	2.0475684271773758E-4	0.0	0.0	3.5370720795275576	2.730091236236501E-4
39	2.0475684271773758E-4	0.0	0.0	3.6533398400507795	2.730091236236501E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21185	0.0	43.003304	1
TAGGGCG	1345	0.0	39.312267	5
TATAGCG	145	0.0	38.793106	1
AGGGCGA	3035	0.0	38.476112	6
GGGCGAT	6035	0.0	38.14002	7
CGTAAGG	455	0.0	38.076923	2
GCGCGAC	1260	0.0	37.32143	9
CGCGACC	1285	0.0	37.120625	10
GTTTTTT	25705	0.0	36.85956	2
CTATGCG	110	0.0	36.81818	1
CGACCCG	1260	0.0	36.785713	32
TGCGTAG	190	0.0	36.710526	1
ACGGGAT	1025	0.0	36.658535	5
ATAGGGC	1590	0.0	36.650944	4
ACCCGCT	1265	0.0	36.64032	34
GGCGATA	1405	0.0	36.512455	8
TGGGCGA	1855	0.0	36.388138	6
TACGCGG	180	0.0	36.25	2
CGACGGT	225	0.0	36.0	28
ACGTATA	25	0.0021076403	36.0	10
>>END_MODULE